Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	630,875	T→C	F328L (TTC→CTC)	cstA →	carbon starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	630,875	0	T	C	100.0%	32.2 / NA	11	F328L (TTC→CTC)	cstA	carbon starvation protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

TCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTT  >  NC_000913/630747‑630979                                                                                                                                 |                                                                                                         tCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTc                                                                                                        >  1:192051/1‑131 (MQ=255)    tGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGtt                                                                                                   >  2:449958/1‑133 (MQ=255)        aaTCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTTGCCCGGTACGGAACGGGAACCTGTTCCCGCTCCTGTTTATc                                                                                               >  2:209740/1‑133 (MQ=255)           cGGGACTATCCTCGGGCTGGCGGTGGGCTTTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCAcc                                                                                            <  2:163459/133‑1 (MQ=255)                   tcgtcgGGCTGGCGGTAGGCACTTTGACTATGCGCCCGACGCTGACCATGCCTGCACTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCtg                                                                                    >  2:295695/1‑133 (MQ=255)                        ggTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGgcg                                                                               <  1:449958/133‑1 (MQ=255)                                            gATTATGCGCTCGACCCTGACCATGCCTTCGCGGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg                                                           <  2:179079/133‑1 (MQ=255)                                                 tGCGCCCGTCGCTGATCATGCCCGCGCTGAGCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                                      <  1:242983/133‑1 (MQ=255)                                                             tGACCATGCCTGCACTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTGCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc                                          <  1:295695/133‑1 (MQ=255)                                                                   tGCCTGCGCTGACCAAATGTGTCGATGGCAGTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc                                    <  1:209740/133‑1 (MQ=255)                                                                    gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg                                   >  1:261796/1‑133 (MQ=255)                                                                         cgcTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGAt                              >  1:427240/1‑133 (MQ=255)                                                                                                     cccGGTATGGACCGGTAACCTGTTCCCGCTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt  <  2:261796/133‑1 (MQ=255)                                                                                                                                 |                                                                                                         TCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTT  >  NC_000913/630747‑630979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑