Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	660,601	A→G	L260L (TTA→CTA)	ybeF ←	putative LysR‑type DNA‑binding transcriptional regulator YbeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	660,601	0	A	G	100.0%	118.3 / NA	34	L260L (TTA→CTA)	ybeF	putative LysR‑type DNA‑binding transcriptional regulator YbeF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

CACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGCTCTTCCGGT  >  NC_000913/660472‑660725                                                                                                                                  |                                                                                                                             cACGCAGGCTCAATTGGTTGTAGTCGATTGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCAt                                                                                                                           <  2:174895/133‑1 (MQ=255)   cGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGt                                                                                                                         >  1:386895/1‑133 (MQ=255)   cGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGt                                                                                                                         >  1:401205/1‑133 (MQ=255)   cGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGt                                                                                                                         >  1:83496/1‑133 (MQ=255)   cGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGt                                                                                                                         >  1:418762/1‑133 (MQ=255)   cGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGt                                                                                                                         >  2:446947/1‑133 (MQ=255)      aGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCAc                                                                                                                      <  1:497915/133‑1 (MQ=255)             aTTTGCTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGc                                                                                                               <  1:342615/133‑1 (MQ=255)               ttgttgTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCaa                                                                                                             <  2:83496/133‑1 (MQ=255)                  ttgtAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTAT‑‑AAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGt                                                                                                        >  2:337894/1‑133 (MQ=255)                               agaAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCAAGTCACTGTTGGCAAACAGTGCGGCGATTGt                                                                                             >  1:248499/1‑133 (MQ=255)                                 aaaTCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCa                                                                                           >  2:130/1‑133 (MQ=255)                                 aaaTCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCa                                                                                           <  2:12240/133‑1 (MQ=255)                                    tCTTTTTGCTCCTCATTTAAGGGCGGAAAAGGCAGTTTTTCCAGCTGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCTTGTGACTGTTGGCAACCCGTGCACCGAGTGTCaaaa                                                                                        >  2:491180/1‑133 (MQ=255)                                        tttGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAAtgtt                                                                                    <  2:510914/133‑1 (MQ=255)                                           gCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTa                                                                                 <  1:411812/133‑1 (MQ=255)                                             tcctcATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGc                                                                               >  2:8345/1‑133 (MQ=255)                                             tcctcATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGACTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGc                                                                               >  1:102306/1‑133 (MQ=255)                                                           cGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATGGTCAAAAGGTTGTAACTGGTGTAATTGAtt                                                                 >  1:143876/1‑133 (MQ=255)                                                            ggAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGAttt                                                                >  1:323627/1‑133 (MQ=255)                                                            ggAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGAttt                                                                >  2:36957/1‑133 (MQ=255)                                                              aaaaGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGc                                                              >  1:64095/1‑133 (MQ=255)                                                              aaaaGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTACAGCGATTGTCATAATATTGTAGCTGGTGATATTGATTTGc                                                              >  2:165730/1‑133 (MQ=255)                                                                       tttttCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGa                                                     <  2:51249/133‑1 (MQ=255)                                                                                    cagcagCGGCTAAACAGGTTATAAAAACGACTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGaa                                        <  2:102306/133‑1 (MQ=255)                                                                                      gcagcGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAAct                                      <  1:446947/133‑1 (MQ=255)                                                                                        agcGGCTAAACAGGTTATTAAATCGGCTGGAAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAActct                                    <  1:165730/133‑1 (MQ=255)                                                                                                 aCAGGTTATAAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAg                           <  1:31679/133‑1 (MQ=255)                                                                                                       tataAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCt                     >  1:315644/1‑133 (MQ=255)                                                                                                        ataAAAACGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTg                    <  2:64095/133‑1 (MQ=255)                                                                                                               cGGCTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAAtttt             >  1:269268/1‑133 (MQ=255)                                                                                                                  cTGGGAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGct          >  2:9207/1‑133 (MQ=255)                                                                                                                     ggAATAATCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGCTCtt       <  2:127323/133‑1 (MQ=255)                                                                                                                       aataatCGCTTGCATCTCACTGTTCGCACCCATTGCAGCGACTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGCTCTTcc     <  2:140693/133‑1 (MQ=255)                                                                                                                        ataatCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGCTCTTCCg    <  1:36957/133‑1 (MQ=255)                                                                                                                          aatCGCTAGCATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGCTCTTCCGGt  <  2:59464/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                             CACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCAGTGCAGCGATTGTCAAAATGTTGTAGCTGGTGAAATTGATTTGCCGGTCCGGAAACATTTCTTGAACTCTCTGCCGCAGACCGCTGAAATTTTGCTCTTCCGGT  >  NC_000913/660472‑660725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑