Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	34,807	T→C	T189A (ACG→GCG)	caiE ←	putative transferase CaiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	34,807	0	T	C	100.0%	95.4 / NA	29	T189A (ACG→GCG)	caiE	putative transferase CaiE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/14);  total (15/14)

AAAGAGTATGCGTCGTTAATTTTATCTCGTTGATACCGGGCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTC  >  NC_000913/34677‑34933                                                                                                                                   |                                                                                                                               aaaGAGTATGCGTCGTTAATTTTATCTCGTTGATACCGGGCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGccc                                                                                                                              >  1:361080/1‑133 (MQ=255)         tGCGTCGTTAATTTTATCTCGTTGATACCGGGCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCaaa                                                                                                                      >  2:387361/1‑133 (MQ=255)         tGCGTCGTTAATTTTATCTCGGTGATACCGAGCGCCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGTAAGATCACAGTTCAGGCGACACATCTGTCGACCCCTGCaaa                                                                                                                      >  2:216403/1‑133 (MQ=255)                        aTCTCGTTGATACCGGGCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTtcc                                                                                                       >  1:505874/1‑133 (MQ=255)                                 aTACCGGGTGTCCTGATTACCAGATGCGATATTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGc                                                                                              <  1:246725/133‑1 (MQ=255)                                       ggCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCGAACAGGGGCGATTTTCGTCCAGTTGCCTCAGt                                                                                        >  2:132140/1‑133 (MQ=255)                                       ggCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGt                                                                                        >  1:49034/1‑133 (MQ=255)                                       ggCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGt                                                                                        >  1:60454/1‑133 (MQ=255)                                         cGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTgg                                                                                      <  2:81381/133‑1 (MQ=255)                                           tCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCt                                                                                    >  1:403439/1‑133 (MQ=255)                                               gCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGt                                                                                >  1:89106/1‑133 (MQ=255)                                                       gATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTa                                                                        >  1:28581/1‑133 (MQ=255)                                                        aTGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTaa                                                                       >  2:405159/1‑133 (MQ=255)                                                        aTGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTaa                                                                       <  2:28581/133‑1 (MQ=255)                                                          gCGATGTTGTCGCATTTTATCCAGCAACAAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAATGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACg                                                                     <  2:214694/133‑1 (MQ=255)                                                                                 gCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGa                                              <  1:413395/133‑1 (MQ=255)                                                                                 gCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGa                                              <  2:89106/133‑1 (MQ=255)                                                                                  cAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTACAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGCCTGCGTTTAATGCAACAATACATGACAGCGCCCGACAAGAt                                             >  2:185340/1‑133 (MQ=255)                                                                                   aaCCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATc                                            >  1:292094/1‑133 (MQ=255)                                                                                   aaCCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATc                                            <  2:403439/133‑1 (MQ=255)                                                                                    aCCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGACACATGACAGCGCCCGACAAGATcc                                           >  1:491755/1‑133 (MQ=255)                                                                                         gTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATa                                      <  1:387361/133‑1 (MQ=255)                                                                                         gTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATa                                      <  1:30447/133‑1 (MQ=255)                                                                                         gTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATa                                      <  2:287276/133‑1 (MQ=255)                                                                                                ccGGCAAGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTg                               <  2:52495/133‑1 (MQ=255)                                                                                                     aaGATCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGACACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTAtt                          <  2:491755/133‑1 (MQ=255)                                                                                                        aTCACCGTTTAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTAATACTCTTTGGTATTCaa                       >  2:422977/1‑133 (MQ=255)                                                                                                               tttAGGCGTCACATCCGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTc                <  1:405159/133‑1 (MQ=255)                                                                                                                  aGGCGTCACATCCGTCGCCCCCCGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTATTTCGTCTTCAACAGTTTCCAg             >  1:221006/1‑133 (MQ=255)                                                                                                                       tCACATCCGTCGCCCCCTGCACACGGGGGCGATTTTCCTCCATTTTCCTCAGTGCCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATCCACTTAGGTATTCAACCGCATCCAGCGTaa        >  2:188268/1‑133 (MQ=255)                                                                                                                             ccGTCGCCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACtcgtc  <  2:361080/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                               AAAGAGTATGCGTCGTTAATTTTATCTCGTTGATACCGGGCGTCCTGCTTGCCAGATGCGATGTTGTAGCATCTTATCCAGCAACCAGGTCGCATCCGGCAAGATCACCGTTTAGGCGTCACATCCGTCGTCCCCTGCAAACGGGGGCGATTTTCCTCCATTTGCCTCAGTGGCTGCGTTTCATGTAACGATACATGACAGCGCCCGACAAGATCCTGATACTCTTTGGTATTCAACCGTTTCCAGTGTAACTCGTC  >  NC_000913/34677‑34933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑