| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 709,068 | G→A | G245G (GGG→GGA) | chiP → | chitobiose outer membrane channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 709,068 | 0 | G | A | 93.3% | 41.5 / ‑5.9 | 15 | G245G (GGG→GGA) | chiP | chitobiose outer membrane channel |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (6/8); minor base T (1/0); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATG > NC_000913/708940‑709195 | cATCTGGAGATGGATAAGTTTTCTCAGAACCATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGAtata < 1:196642/133‑1 (MQ=255) ggAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGAt < 1:404744/133‑1 (MQ=255) ttATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGCCGGAAGGTTATATCGAACAATGTTTTGCCaaa > 2:194601/1‑133 (MQ=255) aaCGATAAAACCACCAAAGTTGATTATCTGCACCCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCt > 2:8819/1‑133 (MQ=255) ccaAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGatat > 2:390377/1‑133 (MQ=255) aCTCCTTTGGGGCGAAATACGCCTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAac < 2:398039/133‑1 (MQ=255) aCTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAac < 1:390377/133‑1 (MQ=255) aCTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAac < 1:8819/133‑1 (MQ=255) tttGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAAccacca < 1:492209/133‑1 (MQ=255) gaggCGAAATCCGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCACTATTCTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGca < 2:432142/131‑2 (MQ=255) ggCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTAc < 1:81899/133‑1 (MQ=255) aaaTACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGt > 1:96341/1‑133 (MQ=255) cAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTAcc > 2:393541/1‑133 (MQ=255) gCATTTGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACCGTACCCGAGATAAAGTTGACGTTCGCAGCg > 1:197544/1‑133 (MQ=255) tGGTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAAc > 2:5928/1‑133 (MQ=255) ggTCAGGCGGAAGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACg < 2:96341/133‑1 (MQ=255) taaGGATATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCGCCAGCTACCAGTTCTACGGTACCCGAGATAAAGGTGACGATCGCAGCGTCAACGACCTTTATg < 2:453263/132‑1 (MQ=255) | CATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAAGGGTATATCGATCAATATTTTGCCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATG > NC_000913/708940‑709195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |