Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	717,696	A→G	S190S (AGT→AGC)	potE ←	putrescine:H(+) symporter/putrescine:ornithine antiporter PotE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	717,696	0	A	G	100.0%	78.6 / NA	24	S190S (AGT→AGC)	potE	putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

CGTACCGCCGAGTACCGCGATTGGCACATTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCACTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGAT  >  NC_000913/717573‑717821                                                                                                                            |                                                                                                                              cGTACCGCCGAGTACCGCGATTGGCACATTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAAc                                                                                                                      <  2:55289/133‑1 (MQ=255)   tACCGCCGAGTACCGCGATTGGCACATTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACgg                                                                                                                    <  2:319122/133‑1 (MQ=255)      cgccgAGTACCGCGATTGGCACATTACGTTCCGGGGTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGc                                                                                                                 >  2:431560/1‑133 (MQ=255)                  cgATTGGCACATTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGAtt                                                                                                     >  2:393330/1‑133 (MQ=255)                       ggCACATTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGATCCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAgg                                                                                                <  1:94992/133‑1 (MQ=255)                         cacaTTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGaa                                                                                              <  2:63951/133‑1 (MQ=255)                            aTTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAAAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCa                                                                                           <  1:355752/133‑1 (MQ=255)                              tACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAAc                                                                                         >  1:361665/1‑133 (MQ=255)                                               aCTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGc                                                                           >  1:464263/1‑130 (MQ=255)                                               aCTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGc                                                                           <  2:464263/130‑1 (MQ=255)                                                      cAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGc                                                                           >  1:455440/1‑123 (MQ=255)                                                      cAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGc                                                                           <  2:455440/123‑1 (MQ=255)                                                        gTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAaaccagaacc                                                               >  1:457602/1‑133 (MQ=255)                                                             cGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCa                                                          >  1:309290/1‑133 (MQ=255)                                                               cacaCGCACACTCCACACCAAGAAAAGCCCTCAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCaat                                                        <  1:132301/133‑1 (MQ=255)                                                                   cGCAGACTCCAGACCAAGAAAAGTCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATg                                                    >  1:117886/1‑133 (MQ=255)                                                                        aCTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAg                                               >  2:306198/1‑133 (MQ=255)                                                                         cTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGa                                              >  1:40700/1‑133 (MQ=255)                                                                            cAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACcg                                           >  2:89620/1‑133 (MQ=255)                                                                                                   tCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCca                    <  2:361665/133‑1 (MQ=255)                                                                                                   tCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCca                    <  2:457602/133‑1 (MQ=255)                                                                                                   tCATAGCGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCca                    <  1:89620/133‑1 (MQ=255)                                                                                                         cGATGGAAGAACCTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGt              <  1:431560/133‑1 (MQ=255)                                                                                                                     cTACCGCGCTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGAt  <  2:309290/133‑1 (MQ=255)                                                                                                                            |                                                                                                                              CGTACCGCCGAGTACCGCGATTGGCACATTACGTTCCGGGTTTTCCACTACATCAGTATTCGCACACGCAGACTCCAGACCAAGAAAAGCCCACAGCGTCATAGCGATGGAAGAACCTACCGCACTGAAGAACGGTGCATGATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGAT  >  NC_000913/717573‑717821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑