| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 764,243 | A→G | T22A (ACT→GCT) | sucD → | succinyl‑CoA synthetase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 764,243 | 0 | A | G | 96.4% | 85.2 / ‑4.6 | 28 | T22A (ACT→GCT) | sucD | succinyl‑CoA synthetase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (10/17); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGC > NC_000913/764120‑764351 | tGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGGTTTCCACt < 2:66695/133‑1 (MQ=255)tGCACCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTCTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACt < 1:84807/133‑1 (MQ=255) cagcaAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACt < 1:1269/131‑1 (MQ=255) cagcaAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACt > 2:1269/1‑131 (MQ=255) cagcaAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCa > 1:420223/1‑133 (MQ=255) cagcaAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCa > 1:496256/1‑133 (MQ=255) aaaGGTCTGATGGATCCAGCTCAGCAGGGTGTGGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACa < 2:149090/133‑1 (MQ=255) ggTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGATTTCCACTCAGAACAGGc > 1:221107/1‑133 (MQ=255) gTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCAAGGGCTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGcc < 2:86477/133‑1 (MQ=255) gATGCAGCTCCGCAGGTTGTTGCCTCAGTGGAAGGGAAATACTGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATa < 2:221107/133‑1 (MQ=255) tGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACg > 2:79312/1‑133 (MQ=255) gttgttGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGt > 2:361259/1‑133 (MQ=255) gCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGt < 2:66199/133‑1 (MQ=255) ttttAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcaccac > 1:514366/1‑133 (MQ=255) ttttAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcaccac > 2:406130/1‑133 (MQ=255) aaTCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCAccacccac < 1:406130/133‑1 (MQ=255) tCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCt < 2:514366/133‑1 (MQ=255) gATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCg > 2:504864/1‑133 (MQ=255) cacCAAGGTTGTCTGCCAGGGCTTCACCTGTATCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 2:219632/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCTGTACCCAGGGGGGTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 2:176083/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTACCCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 2:335067/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 2:339912/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 2:420223/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 2:496256/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 1:73541/133‑1 (MQ=255) cacCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCg < 1:361259/133‑1 (MQ=255) cAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAACGGCGGCAACACCCAGCTCGGCCTGCCGgtg > 1:202642/1‑133 (MQ=255) tCTGCCAGGGTTTTACCGGTAGCCAGGGCGCTGTCCGCTCAAAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCCCCACCCACCTCGGCCTGCCGGTGTTCAacac < 2:202642/133‑1 (MQ=255) tCTGCCAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAacac < 1:79312/133‑1 (MQ=255) cAGGGCTTTACCGGTAGCCAGGGGGCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACcgtgc > 2:314410/1‑133 (MQ=255) | TGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACCAAGGTTATCTGCCAGGGCTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGC > NC_000913/764120‑764351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |