Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 918,591 C→T G177D (GGT→GAT)  ybjX ← DUF535 domain‑containing protein YbjX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913918,5910CT97.1% 112.7 / NA 34G177D (GGT→GAT) ybjXDUF535 domain‑containing protein YbjX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/17);  minor base G (1/0);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTCGTCGCATTCTGGATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATT  >  NC_000913/918465‑918718
                                                                                                                              |                                                                                                                               
gCTTTCGTCGCATTCTGGATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGc                                                                                                                           <  1:307752/133‑1 (MQ=255)
     cgtcgCATTCTGGATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTg                                                                                                                      <  2:97009/133‑1 (MQ=255)
     cgtcgCATTCTGGATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTg                                                                                                                      >  1:402645/1‑133 (MQ=255)
     cgtcgCATTCTGGATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGAAATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTg                                                                                                                      <  1:306598/133‑1 (MQ=255)
                      ccTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCCGCCAGAGGAATAGCTTAGCTGTTGCCGAACAGGATTGTGCt                                                                                                     >  2:156793/1‑133 (MQ=255)
                         gATGTGGACTTTCCCATTTTGCCCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTc                                                                                                  <  1:156793/133‑1 (MQ=255)
                                 aTTTCCCATTATGCGTCTTGCAGTTCGCAAATTAACATCGTCCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACcttctt                                                                                          <  2:184134/133‑1 (MQ=255)
                                    tCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTAt                                                                                       >  2:359230/1‑133 (MQ=255)
                                    tCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTAt                                                                                       <  1:499204/133‑1 (MQ=255)
                                    tCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTAt                                                                                       <  1:458820/133‑1 (MQ=255)
                                             gcgcCTTGCACTCCGCCAATAAACTTAGTTCTGTTCCCCTGGTATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTgaga                                                                              <  2:230546/133‑1 (MQ=255)
                                                            gcattAAAGATCGTTATTCTCCCCTGATATCCCCACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCAGGGTCAGCt                                                               <  1:159636/129‑1 (MQ=255)
                                                            ccAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCt                                                               <  2:402645/133‑1 (MQ=255)
                                                            ccAATAAACACCGTTCTTTTCCCCCGGTTTTCGCACAGGGTCAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCt                                                               <  2:227796/133‑1 (MQ=255)
                                                                         ttCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTg                                                  >  1:349471/1‑133 (MQ=255)
                                                                             tttCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCg                                              <  1:359230/133‑1 (MQ=255)
                                                                                   cTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTGAAGTGTAAACTGCGCGCCGttt                                        >  1:192197/1‑133 (MQ=255)
                                                                                      atatTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTg                                     >  1:508459/1‑133 (MQ=255)
                                                                                      atatTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTg                                     >  2:339544/1‑133 (MQ=255)
                                                                                      atatTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTg                                     >  2:340466/1‑133 (MQ=255)
                                                                                                aGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCt                           >  1:33708/1‑133 (MQ=255)
                                                                                                aGGGTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCt                           >  1:402958/1‑133 (MQ=255)
                                                                                                  ggTAAACGTGATCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGACCTGATCGCCGTTATTGCTTTCCAGCTTc                         >  2:170810/1‑133 (MQ=255)
                                                                                                            aTCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCa               >  1:97364/1‑133 (MQ=255)
                                                                                                            aTCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCa               >  1:336359/1‑133 (MQ=255)
                                                                                                            aTCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCa               >  1:464740/1‑133 (MQ=255)
                                                                                                            aTCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCa               >  1:20577/1‑133 (MQ=255)
                                                                                                            aTCTCTGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCa               >  2:328655/1‑133 (MQ=255)
                                                                                                              ctctGCCAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGTCGTATCTGCCTTCCAGATTCGCAAGTTGCacc             >  2:235421/1‑131 (MQ=255)
                                                                                                                    cAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCgggg       <  1:473913/133‑1 (MQ=255)
                                                                                                                    cAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCgggg       <  2:402958/133‑1 (MQ=255)
                                                                                                                    cAGAGGAATATCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCgggg       <  2:464740/133‑1 (MQ=255)
                                                                                                                         gAATATCTTCGCTGTTGCGGAACATGATTGTGCTGTCACCTCCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAGCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTAtt  <  2:184261/133‑1 (MQ=255)
                                                                                                                         gAATATCTACGCTGTGGCGGAACAGGATTGAGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTAtt  <  1:170810/133‑1 (MQ=255)
                                                                                                                              |                                                                                                                               
GCTTTCGTCGCATTCTGGATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATCCATTGAGATCATCATGGTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATT  >  NC_000913/918465‑918718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑