Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	39,357	T→C	N344D (AAC→GAC)	caiA ←	crotonobetainyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	39,357	0	T	C	100.0%	71.4 / NA	22	N344D (AAC→GAC)	caiA	crotonobetainyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/12);  total (10/12)

GCAGCGCACGGACTTAGCGGTATTGCTTCAGCACCGCACGACCCAGCGTCAGGATCTGCATTTCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTTGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGTTGTCTGCTTTCCACG  >  NC_000913/39231‑39485                                                                                                                               |                                                                                                                                 gCAGCGCATGGACTTTGCGGTAATGCTTCAGCACCGCACGACCCAGCGTCAGGATCTGCATTTCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGc                                                                                                                            <  1:206979/133‑1 (MQ=255)                 gCGGTATTGCTTCAGCACCGCACGACCCAGCGTCAGGATCTGCATTTCGTCAGTTCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGccc                                                                                                            <  2:6090/133‑1 (MQ=255)                      aTTGCTTCAGCACCGCACGACCCAGCGTCAGGATCTGCATTTCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCAc                                                                                                       >  1:73110/1‑133 (MQ=255)                                     cACGACCCAGCGTCAGGATCTGCATTTCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATc                                                                                        >  2:458375/1‑133 (MQ=255)                                     cACGACCCAGCGTCAGGATCTGCATTTCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATc                                                                                        >  2:368798/1‑133 (MQ=255)                                                 tCAGGATCTGTATTTCGTCAGATCTCCCCGAGCTGCAGTCTTCACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCACGCAATCCCGACTCCGCCCAGCCCCTGCATTGCGCTTTCCACAACTCCaaa                                                                            <  2:159073/133‑1 (MQ=255)                                                               tCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGgcgc                                                              >  2:59783/1‑133 (MQ=255)                                                                cGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCa                                                             <  1:128198/133‑1 (MQ=255)                                                                  tCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGa                                                           <  1:368798/133‑1 (MQ=255)                                                                     gATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGt                                                        >  1:461388/1‑133 (MQ=255)                                                                     gATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGt                                                        >  1:339516/1‑133 (MQ=255)                                                                          cccGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTg                                                   <  2:461388/133‑1 (MQ=255)                                                                                                 gATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGATGTATTTGCACATCGATGCATCGCCAGAGGt                            >  1:212933/1‑133 (MQ=255)                                                                                                 gATCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGt                            >  2:490114/1‑133 (MQ=255)                                                                                                   tCACGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGa                          <  1:59783/133‑1 (MQ=255)                                                                                                     aCGCCAGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATg                        <  2:73110/133‑1 (MQ=255)                                                                                                     aCGCCAGAAGCGACTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATg                        >  1:291770/1‑133 (MQ=255)                                                                                                          aGAAGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGcc                   <  1:490114/133‑1 (MQ=255)                                                                                                            aaGCGGCTGATGCGGTGGTCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGt                 >  2:129583/1‑133 (MQ=255)                                                                                                                         ggtggtCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGTTGTCTGCTTTCCa    <  2:339516/133‑1 (MQ=255)                                                                                                                           tggtCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGTTGTCTGCTTTCCACg  <  1:99208/133‑1 (MQ=255)                                                                                                                           tggtCGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGTTGTCTGCTTTCCACg  <  1:129583/133‑1 (MQ=255)                                                                                                                           tggtCGCCCGCAATCCCGACACCGCCCACCACGTGCATTGCCCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGTTGTCTGCTTTCCACg  <  2:212933/133‑1 (MQ=255)                                                                                                                               |                                                                                                                                 GCAGCGCACGGACTTAGCGGTATTGCTTCAGCACCGCACGACCCAGCGTCAGGATCTGCATTTCGTCAGATCCCCCGGAGACGCGGTCTACACGCAGATCACGCCAGAAGCGGCTGATGCGGTGGTTGCCCGCAATCCCGACACCGCCCAGCACCTGCATTGCGCTATCCACAACTTCAAATGCCGCATTGGCGCAGAAGTATTTGCACATCGCTGCATCGCCAGAGGTGATGGTGCCGTTGTCTGCTTTCCACG  >  NC_000913/39231‑39485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑