Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	945,893	T→C	V8A (GTC→GCC)	ycaD →	putative transporter YcaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	945,893	0	T	C	100.0%	93.5 / NA	28	V8A (GTC→GCC)	ycaD	putative transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

ATCGATAATACCGCCGCTGGGATATCGCGTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGTCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGCGTTGTCAGCTCATCCTATT  >  NC_000913/945766‑946021                                                                                                                                |                                                                                                                                 aTCGATAATACCGCCGCTGGGATATCGCGTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAAGTCATGTCGACGAATACCCAGcatgccatgc                                                                                                                             >  1:141229/1‑133 (MQ=255)     ataataCCGCCGCTGGGATATCGCGTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCtttt                                                                                                                         <  2:482092/133‑1 (MQ=255)          aCCGCCGCTGGGATATCGCGTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGt                                                                                                                    <  1:41587/133‑1 (MQ=255)            cgccgcTGGGATATCGCGTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCt                                                                                                                  >  1:393712/1‑133 (MQ=255)                           gcgTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTttgttg                                                                                                   >  2:10391/1‑133 (MQ=255)                           gcgTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCAAGCTTTTGCTGTCGGGCCTGCTTttgttg                                                                                                   >  2:185950/1‑133 (MQ=255)                            cgTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGa                                                                                                  >  2:454394/1‑133 (MQ=255)                                   tCACCCATTGGCAATCTTTTTGGCCGTGCCATCATGCGCATCAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGc                                                                                           <  2:141229/133‑1 (MQ=255)                                     aCCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGa                                                                                         >  2:365884/1‑133 (MQ=255)                                          ttGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCg                                                                                    <  2:99953/133‑1 (MQ=255)                                          ttGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCg                                                                                    <  2:39944/133‑1 (MQ=255)                                                     tttGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAacac                                                                         >  2:364889/1‑133 (MQ=255)                                                       tGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATacac                                                                       >  1:264016/1‑133 (MQ=255)                                                            gTGGCATGCTGCGCATGAAATTCGAACTTCGTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTg                                                                  <  1:197371/133‑1 (MQ=255)                                                            gTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTg                                                                  <  1:370483/133‑1 (MQ=255)                                                            gTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTg                                                                  <  1:368004/133‑1 (MQ=255)                                                             tGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGCTTGCGGTGGTAAACACACTCGTGc                                                                 >  2:197371/1‑133 (MQ=255)                                                                       cgcATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCt                                                       <  1:454394/133‑1 (MQ=255)                                                                       cgcATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCt                                                       >  2:301932/1‑133 (MQ=255)                                                                          aTGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGc                                                    <  2:393712/133‑1 (MQ=255)                                                                           tGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGcc                                                   <  2:264016/133‑1 (MQ=255)                                                                                           tCACGGTTTTAATTCATGTCCAAGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCca                                   <  1:185950/133‑1 (MQ=255)                                                                                               ggTTTTAATTCATGTCCACGTATACCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATg                               <  1:85490/133‑1 (MQ=255)                                                                                                         cATGTCCATGTATACCCATCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGc                     <  1:216516/133‑1 (MQ=255)                                                                                                                      aCCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGCGTTGTCAGCTCAt        >  2:34815/1‑133 (MQ=255)                                                                                                                      aCCCAGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGCGTTGTCAGCTCAt        >  1:498782/1‑133 (MQ=255)                                                                                                                          aGCCTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCAAATGGCAGCTAGTCGTTGTCAGATCATCCTa    >  1:207034/1‑133 (MQ=255)                                                                                                                            ccTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGCGTTGTCAGCTCATCCTAtt  >  2:375231/1‑133 (MQ=255)                                                                                                                            ccTGCCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGCGTTGTCAGCTCATCCTAtt  >  2:255691/1‑133 (MQ=255)                                                                                                                                |                                                                                                                                 ATCGATAATACCGCCGCTGGGATATCGCGTATTTTCACCCATTGACAATGTTTTTGGCGGTGGCATGATGCGCATGAAATTTGAACTTCCTCACGGTTTTAATTCATGTCCACGTATACCCAGCCTGTCATGCTTTTGCTGTCTGGCCTGCTTTTGTTGACTCTGGCGATTGCGGTGTTAAATACACTCGTGCCGCTTTGGCTCGCCCAGGAACACATGTCCACATGGCAGGTAGGCGTTGTCAGCTCATCCTATT  >  NC_000913/945766‑946021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑