Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	952,608	A→G	V316A (GTT→GCT)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	952,608	0	A	G	100.0%	65.4 / NA	20	V316A (GTT→GCT)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

GGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAA  >  NC_000913/952483‑952726                                                                                                                              |                                                                                                                       ggAAACGGAATCTGTATTTGGTAACCAGTGTAAGACCGTCGTGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACg                                                                                                                 <  2:136465/133‑1 (MQ=255)                ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAg                                                                                                  <  1:341858/133‑1 (MQ=255)                                aCGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTcc                                                                                  >  1:453897/1‑133 (MQ=255)                                      gTCGAGGCCCATACCACCGATAGATTCGGATGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGct                                                                            >  2:302858/1‑133 (MQ=255)                                                                      cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc                                            <  2:366444/133‑1 (MQ=255)                                                                      cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc                                            >  1:514494/1‑133 (MQ=255)                                                                          gATCGGGTCGCCAGAAAACAGTTCCTCGTATTTCGGAGTACCCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGt                                        <  2:148497/133‑1 (MQ=255)                                                                              gggTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGa                                    <  1:72110/133‑1 (MQ=255)                                                                                  cGCCAGAGTACAGTTCATAGTAGTCCGGAGGACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTa                                <  1:216871/133‑1 (MQ=255)                                                                                  cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTa                                <  2:453897/133‑1 (MQ=255)                                                                                     cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTCCAGGTCACGTTCGATGTacac                             >  1:331880/1‑133 (MQ=255)                                                                                     cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTCGCCAGCTATCAAGTGACGTTCAATGTacac                             >  1:163521/1‑133 (MQ=255)                                                                                               ttCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAg                   >  2:115714/1‑133 (MQ=255)                                                                                               ttCATCGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAg                   >  2:118460/1‑133 (MQ=255)                                                                                                    cGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGa                     <  1:437738/126‑1 (MQ=255)                                                                                                    cGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGa                     >  2:437738/1‑126 (MQ=255)                                                                                                    cGTATTCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGa              >  1:297884/1‑133 (MQ=255)                                                                                                        ttCCGGAGTACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTa          >  1:455567/1‑133 (MQ=255)                                                                                                                tACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGaa  >  1:412152/1‑133 (MQ=255)                                                                                                                tACGCAGGAAGCGAGCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGaa  <  2:455567/133‑1 (MQ=255)                                                                                                                              |                                                                                                                       GGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAA  >  NC_000913/952483‑952726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑