Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,017,932	A→G	V123A (GTA→GCA)	ycbZ ←	putative ATP‑dependent protease YcbZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,017,932	0	A	G	100.0%	52.3 / NA	16	V123A (GTA→GCA)	ycbZ	putative ATP‑dependent protease YcbZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/6);  total (10/6)

TACGCAAAGAGATAATGAGAATACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTACCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATC  >  NC_000913/1017822‑1018053                                                                                                               |                                                                                                                          tACGCAAAGAGATAATGAGAATACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTg                                                                                                     >  1:33735/1‑133 (MQ=255)                   gaATACCGCCATTTGCCTGGTGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAAtt                                                                                   <  2:3995/133‑1 (MQ=255)                   gaATACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAAtt                                                                                   <  2:356750/133‑1 (MQ=255)                    aaTACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTa                                                                                  >  1:382078/1‑133 (MQ=255)                    aaTACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTa                                                                                  >  2:278475/1‑133 (MQ=255)                     aTACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTat                                                                                 >  2:343128/1‑133 (MQ=255)                           ccATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTg                                                                           >  1:415940/1‑133 (MQ=255)                             aTTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGca                                                                         <  2:64450/133‑1 (MQ=255)                              tttGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGcac                                                                        >  1:79655/1‑133 (MQ=255)                              tttGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGcac                                                                        >  1:87600/1‑133 (MQ=255)                                                 cagGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCg                                                     >  1:87968/1‑133 (MQ=255)                                                    gCTGCAGGGTAATGTCGCCACTACACTGGCGCACGCAGCCAAAGAGTTGCTCCGCTTCTACCCCGTCGTCAGCGTCAACTTGCGTTCAAACCGCAAAATGATCATCTGCACTCACTGCCTGACGTAAGCGGag                                                  >  2:363275/1‑132 (MQ=255)                                                                tGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGa                                      <  2:33735/133‑1 (MQ=255)                                                                 gTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGaa                                     <  2:415940/133‑1 (MQ=255)                                                                  tCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGaaa                                    >  1:438258/1‑133 (MQ=255)                                                                   cGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAAc                                   <  2:87968/133‑1 (MQ=255)                                                                      cATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTc                                >  1:436227/1‑133 (MQ=255)                                                                                                    gCTCCGCTTCTGCCCAGTCGGCAGCGACAACTTGCGGTAAACACGCAAAATTATCATCTGCACTAACTGCGTGACGTGAGCGGATGGAGGGGCCGGAAACCTCATAGACACCGACCACCAGTTTGCCTGCATc  >  2:55467/1‑133 (MQ=255)                                                                                                               |                                                                                                                          TACGCAAAGAGATAATGAGAATACCGCCATTTGCCTGATGCACCAGACCAGGCTGCAGGGTAATGTCGCCATTAAACTGGCGCAGGCAGCCAAAGAGTTGCTCCGCTTCTACCCAGTCGGCAGCGACAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATC  >  NC_000913/1017822‑1018053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑