| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,023,368 | A→G | R172R (CGT→CGC) | yccS ← | putative transporter YccS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,023,368 | 0 | A | G | 97.4% | 120.6 / ‑4.2 | 39 | R172R (CGT→CGC) | yccS | putative transporter YccS |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (20/17); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
GATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAT > NC_000913/1023238‑1023494 | gATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGcg > 1:46929/1‑133 (MQ=255) ttCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGcgcg < 2:46929/133‑1 (MQ=255) gCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGt > 1:363602/1‑133 (MQ=255) gACCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAgcgg > 1:480684/1‑133 (MQ=255) aCCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAgcggg > 1:495697/1‑133 (MQ=255) cGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCCCCACGTAGTCCTGTAgcgggcg > 1:209692/1‑133 (MQ=255) ttGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGa < 1:451481/133‑1 (MQ=255) tCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGAAACAGCAGAt < 2:471342/133‑1 (MQ=255) tccAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCGAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGAc < 2:391523/131‑1 (MQ=255) aGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCaa < 1:479695/133‑1 (MQ=255) gTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAg > 2:98133/1‑133 (MQ=255) ccTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACTGGGAACAGCATATGAACAATAAgtgt > 2:229522/1‑133 (MQ=255) ggCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAg < 2:56106/133‑1 (MQ=255) cTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGa > 1:383483/1‑133 (MQ=255) tGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGCGCGGACCGAGAAGAGCAGAGGACCAATAAGGTTCAGGAcct > 2:246450/1‑131 (MQ=255) tatCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGAc > 2:286727/1‑133 (MQ=255) tCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACgg > 2:62335/1‑133 (MQ=255) tCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACgg > 1:287593/1‑133 (MQ=255) aTCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCg > 1:272946/1‑133 (MQ=255) aTCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCg > 1:16306/1‑133 (MQ=255) cAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGc < 2:340109/133‑1 (MQ=255) aaaCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGcc < 2:363602/133‑1 (MQ=255) aaaCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGcc < 2:495697/133‑1 (MQ=255) aaaCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGAAGTTGTACCAGACGGCACCGGcc > 1:184031/1‑133 (MQ=255) aCATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCcag > 2:362603/1‑133 (MQ=255) gcgcGACTTGAGCTCAAGATAACGCGCCAGATGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGTGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGa > 2:434490/1‑133 (MQ=255) cgcgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGAt > 1:89236/1‑133 (MQ=255) cgcgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGAt > 1:411063/1‑133 (MQ=255) gcgACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATa > 2:499330/1‑133 (MQ=255) cTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATc > 1:5561/1‑133 (MQ=255) tGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCgg < 1:21705/133‑1 (MQ=255) aGCTCAAGATAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGgct < 2:89236/133‑1 (MQ=255) tAACGCGCCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATAcc < 1:98133/133‑1 (MQ=255) gcgcCAGTTGTTCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTg < 2:446393/133‑1 (MQ=255) cAGTTGTTCATAGCATCGCGCCAGTTTGTCCTGCAGCGGGCCGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCa < 2:209692/133‑1 (MQ=255) tCATAGCCGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTg < 2:480684/133‑1 (MQ=255) tCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTg < 2:287593/133‑1 (MQ=255) tCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTg < 2:418103/133‑1 (MQ=255) tCATAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTg < 1:362603/133‑1 (MQ=255) aTAGCAGCGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAt < 1:286727/133‑1 (MQ=255) | GATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGTGCCTGGCTTTGATCTTCAATATCAGGATCAAACATGCGCGACTTGAGCTCAAGATAACGCGCCAGTTGTTCATAGCAACGCGCCAGGTTGTCCTGCAGCGGGCGGACCGGGAACAGCAGATGACCAATAAGTGTCAGGACGTTGTACCAGACGGCACCGGCCAGCAGATACATCGGCTGCTGATACCAGTGCTCATACAGTGAT > NC_000913/1023238‑1023494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |