Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,045,590	C→T	A72T (GCT→ACT)	gfcE ←	putative exopolysaccharide export lipoprotein GfcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,045,590	0	C	T	100.0%	130.3 / NA	40	A72T (GCT→ACT)	gfcE	putative exopolysaccharide export lipoprotein GfcE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/16);  total (24/16)

GGCGCTACGGTATTGCCCTGCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACT  >  NC_000913/1045465‑1045710                                                                                                                              |                                                                                                                         ggCGCTACGGTATTGCCCTGCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGAc                                                                                                                   >  1:117521/1‑133 (MQ=255)     cTACGGTATTGCCCTGCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATaa                                                                                                               <  1:258290/133‑1 (MQ=255)             ttGCCCTGCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATTATCGGTTTg                                                                                                       >  2:315545/1‑133 (MQ=255)               gCCCTGCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGgc                                                                                                     >  1:372954/1‑133 (MQ=255)                cccTGCGGGGGTGGTGAGTTCCGGGTGGTCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGgcg                                                                                                    >  1:80251/1‑133 (MQ=255)                    gCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCa                                                                                                >  1:476967/1‑133 (MQ=255)                     cGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCaa                                                                                               <  2:372954/133‑1 (MQ=255)                       gcggtggtTATTTCCTGGTGATCTGAGATCGTCACCATCAATACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACt                                                                                             <  2:249963/131‑1 (MQ=255)                         ggtggtTAGTCCCGGGTGATCCCAAACCCTAATCATCAACACATCACCAATACCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGa                                                                                           <  2:204035/133‑1 (MQ=255)                              ttAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGatcaat                                                                                      <  2:117521/133‑1 (MQ=255)                                    ccGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATcc                                                                                >  2:472142/1‑133 (MQ=255)                                       ggTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTgg                                                                             >  2:513744/1‑133 (MQ=255)                                            tCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGa                                                                        <  2:308574/133‑1 (MQ=255)                                             cccAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGAc                                                                       <  1:464335/133‑1 (MQ=255)                                                 gACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGga                                                                   <  1:420976/133‑1 (MQ=255)                                                      tAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGgatagat                                                              >  2:356857/1‑133 (MQ=255)                                                      tAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGACGTGACGGgatagat                                                              >  1:226621/1‑133 (MQ=255)                                                      tAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTCATCAATCAATCATGTCGTGACAGgatagat                                                              >  1:221306/1‑133 (MQ=255)                                                           aTCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGa                                                         <  2:476967/133‑1 (MQ=255)                                                             cAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGAcc                                                       >  2:439260/1‑133 (MQ=255)                                                                cacaTCACTAATCCCGATGCGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCAtt                                                    <  1:219712/133‑1 (MQ=255)                                                                   aTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTg                                                 >  1:46014/1‑133 (MQ=255)                                                                   aTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGAGCAATCAATGCTGGCGTGACGGGATAGATATGGACCATTTTg                                                 >  2:130993/1‑133 (MQ=255)                                                                   aTCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCAAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTg                                                 >  1:363677/1‑133 (MQ=255)                                                                    tCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGt                                                <  2:10536/133‑1 (MQ=255)                                                                     cACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCTATCAATCCTGGCCTGACGGGATAGAAATTGACCATTTTGTc                                               >  2:174663/1‑133 (MQ=255)                                                                     cACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTc                                               >  2:86736/1‑133 (MQ=255)                                                                      aCCAATCCCGATACGCTATTCATACTTTACGAGTTGCTGCTGCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTcc                                              <  2:184322/133‑1 (MQ=255)                                                                              cgatacgataTTCATTGTTTGCGAGTGGCTGCGCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATa                                      <  2:226621/133‑1 (MQ=255)                                                                               gatacgataTTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTTGCGTGACGAGATTGATCTTGACCATTTTGTTCAGGTCATAc                                     >  2:157326/1‑133 (MQ=255)                                                                                 tacgataTTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATGGATATTGCCCATTTAGTCCAGATCATACTg                                   >  1:184322/1‑133 (MQ=255)                                                                                         tCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAg                           >  2:4909/1‑133 (MQ=255)                                                                                          cATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAgg                          <  2:46014/133‑1 (MQ=255)                                                                                          cATAGTTTACGATTTACTTCTCTAGCTCCGCGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAgg                          <  1:130993/133‑1 (MQ=255)                                                                                              gTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGCTCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAga                      >  2:27879/1‑132 (MQ=255)                                                                                                  gCGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCaa                  >  1:113920/1‑133 (MQ=255)                                                                                                   cGATTTGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGCCATACTGATTGTCAGGTAGTTCAAt                 >  1:468970/1‑133 (MQ=255)                                                                                                       ttGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGAc              >  1:75823/1‑132 (MQ=255)                                                                                                       ttGCTGCTCCAGCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGAc              <  2:75823/132‑1 (MQ=255)                                                                                                                  gCTCCGGGTTAGTCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGCTTGACAGGTAGTTCAATGACACCCTTGTTACt  >  2:185008/1‑133 (MQ=255)                                                                                                                              |                                                                                                                         GGCGCTACGGTATTGCCCTGCGGGGGTGGTTAGTTCCGGGTGATCCCAGACCGTAACCATCAACACATCACCAATCCCGATACGATATTCATAGTTTGCGATTTGCTGCTCCAGCTCCGGGTTAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACT  >  NC_000913/1045465‑1045710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑