| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,079 | T→C | I158V (ATC→GTC) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,079 | 0 | T | C | 92.0% | 68.9 / ‑3.6 | 25 | I158V (ATC→GTC) | araD | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (8/15); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
AATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGT > NC_000913/65955‑66202 | aaTATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGtt > 2:375957/1‑133 (MQ=255) ccccATATCAGCGACCTCTTCCAGCACGATCGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCa < 1:131943/133‑1 (MQ=255) ccccATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCa < 1:364952/133‑1 (MQ=255) cccATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCaa < 1:398583/133‑1 (MQ=255) aGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCt > 2:47235/1‑133 (MQ=255) aGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCt > 1:417628/1‑133 (MQ=255) aCCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCACCGACACCTTGTCTTTCAAAGGCTTCTACGa > 1:216775/1‑133 (MQ=255) ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGAt < 2:481630/133‑1 (MQ=255) ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGAt > 1:473264/1‑133 (MQ=255) ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGAt > 1:271692/1‑133 (MQ=255) ttCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACg < 2:390527/133‑1 (MQ=255) cACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCg > 1:113357/1‑133 (MQ=255) cACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCACATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCg < 2:384610/133‑1 (MQ=255) gATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGtt < 2:113357/133‑1 (MQ=255) tGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTc > 1:57422/1‑133 (MQ=255) cACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCatat < 2:422917/133‑1 (MQ=255) ttttGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGc < 2:417628/133‑1 (MQ=255) ttGCCCCATGAAAACGGGCCGTGGTAATCGACCAGAACGCCCGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGt < 2:216775/133‑1 (MQ=255) aCGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCg < 1:413130/133‑1 (MQ=255) cGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCgg < 1:339373/133‑1 (MQ=255) gCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTg < 1:47235/133‑1 (MQ=255) ccGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGc > 2:355207/1‑133 (MQ=255) gAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGaa < 2:271692/133‑1 (MQ=255) ggACCAGAACGCCGGGCATTTGCGCTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGt < 1:375957/133‑1 (MQ=255) cTGCATCGACACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCgtgggt < 2:320377/133‑1 (MQ=255) | AATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGT > NC_000913/65955‑66202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |