Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,059,367	A→G	T38A (ACT→GCT)	torA →	trimethylamine N‑oxide reductase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,059,367	0	A	G	100.0%	80.6 / NA	24	T38A (ACT→GCT)	torA	trimethylamine N‑oxide reductase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/9);  total (15/9)

ATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGAACT  >  NC_000913/1059237‑1059491                                                                                                                                   |                                                                                                                             aTAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGTGGCGGTCTTTGTTAACCCCGCGACGTGCGGCt                                                                                                                            >  2:174822/1‑133 (MQ=255) aTAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCt                                                                                                                            >  2:7246/1‑133 (MQ=255) aTAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCt                                                                                                                            >  2:108621/1‑133 (MQ=255)         gaagaaAAATAATGACTAATAACGATCTCTTTCAGGCAGCACGTCGGCGATTTGTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGGGCGGCTGCGCCGCa                                                                                                                    >  2:504097/1‑133 (MQ=255)           agaaAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAg                                                                                                                  <  1:312600/133‑1 (MQ=255)                   aatGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACt                                                                                                          <  1:7246/133‑1 (MQ=255)                       aaCAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGATGTGCGGCTGCGCCGCAAGCGGAGACTGACg                                                                                                      >  1:188833/1‑133 (MQ=255)                                      tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAag                                                                                       >  2:9348/1‑133 (MQ=255)                                      tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAag                                                                                       >  1:478254/1‑133 (MQ=255)                                      tttCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGAAAGCGGCGACTGACGCTGTCAACTCGAAag                                                                                       >  1:154655/1‑133 (MQ=255)                                        tCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAagag                                                                                     >  1:479272/1‑133 (MQ=255)                                          aGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAggg                                                                                   <  1:345499/133‑1 (MQ=255)                                              aTCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCAtt                                                                               >  1:382/1‑133 (MQ=255)                                              aTCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCAtt                                                                               >  2:121101/1‑133 (MQ=255)                                                cACGACGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTTAAGGTGTCATCTCGAAAGAGGGCATTCt                                                                             >  2:240377/1‑133 (MQ=255)                                                    tCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTAcc                                                                         <  1:108621/133‑1 (MQ=255)                                                    tCGGCGTTATCTGGCCCACCTCTGCGGCTTTACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTAcc                                                                         <  1:174822/133‑1 (MQ=255)                                                                             ggcTTAACTGTCGCCAGGCTGCGGGGGCCGACATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCcgcg                                                <  1:240377/133‑1 (MQ=255)                                                                                      gTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGa                                       <  2:479272/133‑1 (MQ=255)                                                                                         gCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAgg                                    >  1:73031/1‑133 (MQ=255)                                                                                         gCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAgg                                    >  1:261266/1‑133 (MQ=255)                                                                                                                         gcgACGTGCGGCTGCGGCGCTAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGaa    >  1:58138/1‑133 (MQ=255)                                                                                                                         gcgACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGaa    >  1:262587/1‑133 (MQ=255)                                                                                                                         gcgACGTGCGGCTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGaa    <  2:264462/133‑1 (MQ=255)                                                                                                                           gACGTGCGGCCGCGGCGCAAGCGGCGACCGACCCTGTCATCCCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGAACt  <  2:188833/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                             ATAAGAAGGAAGAAAAATAATGAACAATAACGATCTCTTTCAGGCATCACGTCGGCGTTTTCTGGCACAACTCGGCGGCTTAACCGTCGCCGGGATGCTGGGGCCGTCATTGTTAACGCCGCGACGTGCGACTGCGGCGCAAGCGGCGACTGACGCTGTCATCTCGAAAGAGGGCATTCTTACCGGGTCGCACTGGGGGGCTATCCGCGCGACGGTGAAGGATGGTCGCTTTGTGGCGGCAAAACCGTTCGAACT  >  NC_000913/1059237‑1059491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑