| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,110,661 | A→G | T443A (ACC→GCC) | opgG → | osmoregulated periplasmic glucans (OPGs) biosynthesis protein G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,110,661 | 0 | A | G | 94.7% | 59.1 / NA | 19 | T443A (ACC→GCC) | opgG | osmoregulated periplasmic glucans (OPGs) biosynthesis protein G |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (6/12); minor base T (1/0); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGTGCGCTATAACCCGGTTACCAAAGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAA > NC_000913/1110536‑1110774 | gggTGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGt > 1:255824/1‑133 (MQ=255) ggTGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAGACTGCCAGAGGATTCCCCGGTc > 1:160358/1‑133 (MQ=255) ggTGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTc > 2:502183/1‑133 (MQ=255) gTGCAACAGACGCGTTGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTca < 1:234603/133‑1 (MQ=255) gTGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTca < 1:435804/133‑1 (MQ=255) tGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTcac > 2:24015/1‑133 (MQ=255) cgcgTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGATATGAAAAAACTGTCAGAGGATGCCCGGGTCGCAGCGCAAAc > 2:170248/1‑133 (MQ=255) cgtTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCa < 1:392121/133‑1 (MQ=255) cgtTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCa < 2:72544/133‑1 (MQ=255) cgtTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTAACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCa < 1:258622/133‑1 (MQ=255) cgtTCAACCGGGGATTTGTAGCCGTCGAACATGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCa < 1:170248/133‑1 (MQ=255) gggggATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCATTGGTGAt < 2:255824/133‑1 (MQ=255) aGTCGAACCTGATTCGCCAGCCTGACGATACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCTAACCAGCATTGATGATAATGGGGAGATAGt > 1:145846/1‑133 (MQ=255) cTGATTCGTCAGCCTTACAGTATTATCGCCGTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCa < 1:207756/133‑1 (MQ=255) ttCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGt < 1:502183/133‑1 (MQ=255) ccTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGTGCGCTATAACCCGGTTACCAAAgg < 1:335603/133‑1 (MQ=255) cgcAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGTGCGCTATAACCCGGTTACCAAAGGCTGGCGTCTGGTGATGCGtgtg < 1:448592/133‑1 (MQ=255) cgcAGAGATGAAAAAACTGCCAGAGGATGCCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGTGCGCTATAACCCGGTTACCAAAGGCTGGCGTCTGGTGATGCGtgtg > 2:73931/1‑133 (MQ=255) gAAAAAACTGTCAGAGGATGCCCCGGTCACAGGGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGTGCGCTATAACCCGGTTACCAAAGGCTGGCGTCTGGTGATGCGTgtgaaagtgaaa < 2:160358/133‑1 (MQ=255) | GGGTGCAACAAACGCGTCGTTCAACGGGGGATGTGAAGCAGTCGAACCTGATTCGCCAGCCTGACGGTACTATCGCCTTTGTGGTCGATTTTACCGGCGCAGAGATGAAAAAACTGCCAGAGGATACCCCGGTCACAGCGCAAACCAGCATTGGTGATAATGGTGAGATAGTTGAAAGCACGGTGCGCTATAACCCGGTTACCAAAGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAA > NC_000913/1110536‑1110774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |