Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,120,223	T→C	I122V (ATT→GTT)	solA ←	N‑methyl‑L‑tryptophan oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,120,223	0	T	C	100.0%	57.6 / NA	18	I122V (ATT→GTT)	solA	N‑methyl‑L‑tryptophan oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

TTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAT  >  NC_000913/1120116‑1120345                                                                                                            |                                                                                                                           ttGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACg                                                                                                   <  1:289994/133‑1 (MQ=255)      tCCAGGTTGTAATCGCCAGTTCGCTGCGCATAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAg                                                                                              <  1:232487/133‑1 (MQ=255)           gTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTCGCGCATCTAGCTTTTCAACGTAGAGTTGcc                                                                                         >  1:172746/1‑133 (MQ=255)            ttttAATCGCCAGTTCGCTGCGCAAAAAGCCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCa                                                                                        <  2:358183/133‑1 (MQ=255)                 aTCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGtt                                                                                   <  1:51599/133‑1 (MQ=255)                                           ggAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCgaga                                                         <  1:429845/133‑1 (MQ=255)                                               tCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAAtg                                                     <  1:418081/133‑1 (MQ=255)                                                             gCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAATCAGCCggg                                       >  1:96549/1‑133 (MQ=255)                                                               cGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGcc                                     <  1:477717/133‑1 (MQ=255)                                                               cGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGcc                                     <  2:124807/133‑1 (MQ=255)                                                                        tGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAAt                            >  1:444679/1‑133 (MQ=255)                                                                           ccGGGACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGAc                         >  2:421345/1‑133 (MQ=255)                                                                               gACGCGTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCa                     >  2:289049/1‑133 (MQ=255)                                                                                 cgcgTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCaga                   >  2:408413/1‑133 (MQ=255)                                                                                  gcgTATTTCCGGCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCagag                  <  1:408413/133‑1 (MQ=255)                                                                                             gCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAAt       <  2:443990/133‑1 (MQ=255)                                                                                             gCCAGCGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAAt       >  1:443990/1‑133 (MQ=255)                                                                                                  cGGGCCATAACCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAt  >  1:21770/1‑133 (MQ=255)                                                                                                            |                                                                                                                           TTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAT  >  NC_000913/1120116‑1120345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑