Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,132,417 T→C G188G (GGT→GGC flgD → flagellar biosynthesis, initiation of hook assembly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,132,4170TC95.0% 58.3 / ‑3.8 21G188G (GGT→GGCflgDflagellar biosynthesis, initiation of hook assembly
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (8/11);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

ATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTCGACGAAGTACGGCAGA  >  NC_000913/1132307‑1132541
                                                                                                              |                                                                                                                            
atTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGc                                                                                                        <  2:261381/133‑1 (MQ=255)
   ggTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATTTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGc                                                                                                     >  1:328943/1‑133 (MQ=255)
   ggTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGc                                                                                                     >  1:454475/1‑133 (MQ=255)
            aCCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTg                                                                                            <  2:515917/133‑1 (MQ=255)
              cgccgGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGct                                                                                          <  2:454475/133‑1 (MQ=255)
                ccgGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGctct                                                                                        <  2:483363/133‑1 (MQ=255)
                    aGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTg                                                                                    <  1:384036/133‑1 (MQ=255)
                     gTTCGCAGGTTCACCTGGAACGCTACGTTGTCTGATGGCAGCACTGCGCCGAACGGTTTTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGc                                                                                   <  1:179847/133‑1 (MQ=255)
                     gTTCACAGTTTCACCAGGGACGGTCCGTAGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGc                                                                                   <  2:140690/133‑1 (MQ=255)
                                       gACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCa                                                                 <  1:296727/133‑1 (MQ=255)
                                       gACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCa                                                                 >  2:296727/1‑133 (MQ=255)
                                        aCGGTACGTTGACTGATGGCAGCATTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCaa                                                                <  1:372734/133‑1 (MQ=255)
                                            tACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGc                                                            >  2:115242/1‑133 (MQ=255)
                                                    cTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATAc                                                    >  2:296655/1‑133 (MQ=255)
                                                      gATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGc                                                  >  2:74906/1‑133 (MQ=255)
                                                      gATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGGGTGATCCGCGGCAACAGCGGTAATACGc                                                  >  2:389566/1‑133 (MQ=255)
                                                                       gAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTAc                                 <  1:389566/133‑1 (MQ=255)
                                                                                      tGTAGCGATTAGCGCCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCCCGGCAACAGCAGTAATACGTTGGATCTGGGCACTTAAGGCAGCACCACCCTc                  >  2:206429/1‑133 (MQ=255)
                                                                                          gCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTcgacg              >  2:66759/1‑133 (MQ=255)
                                                                                                  cgcCAGTAACGGCGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTCGACGAAGTACgg      <  1:74906/133‑1 (MQ=255)
                                                                                                      aGTAACGGGGGTACACAACTGGTTGCCCAGTCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTCGACGAAGTACGGCAGa  <  1:206429/133‑1 (MQ=255)
                                                                                                              |                                                                                                                            
ATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTCGACGAAGTACGGCAGA  >  NC_000913/1132307‑1132541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: