| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,168,020 | G→A | G141D (GGT→GAT) | ycfJ → | PF05433 family protein YcfJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,168,020 | 0 | G | A | 86.4% | 57.9 / NA | 22 | G141D (GGT→GAT) | ycfJ | PF05433 family protein YcfJ |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (9/10); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATAACAAGGCTGTA > NC_000913/1167893‑1168148 | gttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTatg < 2:469979/133‑1 (MQ=255) gtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAACATGATCGGTTATGATGGGACCGATAAGATTgg > 2:142020/1‑133 (MQ=255) gCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAATAATGCTCGGTTACGATGTGACCTATGAGATGGGCGATcagc > 2:140718/1‑133 (MQ=255) cAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCa > 1:27013/1‑133 (MQ=255) ctctcCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGCCGATCAGAAGGTCATAATCCGCATGGACcg > 1:240534/1‑133 (MQ=255) gggATACTTGTACGACTACGCAACAGTGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTAc < 2:240534/131‑1 (MQ=255) cacGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCg < 2:5662/133‑1 (MQ=255) cacGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCg < 2:27013/133‑1 (MQ=255) aCTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAg > 2:288834/1‑133 (MQ=255) cGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGTGTACGCACATCACGCTAGATAGCAACGGGc > 1:206342/1‑133 (MQ=255) tgtATGACCAGTCAGCAAAAATACTCGATTCTGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGaa < 1:140718/133‑1 (MQ=255) tgtATGACAAGTCAGCAAAAATGCCCGAGTATAATGTGACCCATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGaa < 2:206342/133‑1 (MQ=255) tGACAAGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAAc < 2:324864/133‑1 (MQ=255) aGTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGt > 2:9029/1‑133 (MQ=255) gTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGta > 1:449236/1‑133 (MQ=255) gTCAGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAAAAAAGta > 2:363329/1‑133 (MQ=255) aGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATaa < 1:9029/133‑1 (MQ=255) aGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATaa > 1:74208/1‑133 (MQ=255) aGAAAAAATGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATaa < 1:288834/133‑1 (MQ=255) agaTGCTCGGTTATGATGTGACCCATGATATTTGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATAACAAgg < 2:244014/131‑1 (MQ=255) aaaTGCTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCTACTGATTCTGAGTATCAAAGTGTAACAAgg > 1:244014/1‑133 (MQ=255) cTCGATTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATAACAAGGCTGTa < 2:74208/133‑1 (MQ=255) | GTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGATAGCAACGGGCAACTGATTTTGAATAACAAAGTATAACAAGGCTGTA > NC_000913/1167893‑1168148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |