Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,190,998	A→C	V330G (GTT→GGT)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,190,998	0	A	C	100.0%	72.4 / NA	23	V330G (GTT→GGT)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/10);  total (13/10)

ACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCATGGTGGAAGAACCAATCT  >  NC_000913/1190874‑1191112                                                                                                                             |                                                                                                                   aCGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGTTAGAGTCGATCAAGTCTCGCTGCCAACGGGAACCCGGCAGt                                                                                                            >  2:222769/1‑133 (MQ=255)          ttCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGcc                                                                                                   >  2:2813/1‑133 (MQ=255)          ttCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGCTCAGGTCACGCTGCCAACGGGAACCAGGCAGTTTGCTTGcc                                                                                                   >  2:186208/1‑133 (MQ=255)             cAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGa                                                                                                <  2:128278/133‑1 (MQ=255)                    cTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCa                                                                                         >  1:93521/1‑133 (MQ=255)                                   gTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGaaa                                                                          >  1:15822/1‑133 (MQ=255)                                   gTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGaaa                                                                          <  1:277498/133‑1 (MQ=255)                                       aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGcc                                                                      >  2:467689/1‑133 (MQ=255)                                                cAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTccc                                                             >  1:14332/1‑133 (MQ=255)                                                    cAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCg                                                         >  2:306157/1‑133 (MQ=255)                                                             aCCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCg                                                >  1:356884/1‑133 (MQ=255)                                                                    cccACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGa                                         <  1:467689/133‑1 (MQ=255)                                                                     ccACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGAt                                        <  2:93521/133‑1 (MQ=255)                                                                     ccACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGAt                                        <  2:356884/133‑1 (MQ=255)                                                                     ccACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGAt                                        <  2:15822/133‑1 (MQ=255)                                                                     ccACGCCGAGGTTACACAGCACTGTAGAGTAGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGAt                                        <  1:202173/133‑1 (MQ=255)                                                                                        caCGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCTCTTCGGAGCTTTAGAAATCGATCGGCTTAAGGTTATGCGGc                     >  2:226797/1‑133 (MQ=255)                                                                                         aCGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCa                    <  2:337757/133‑1 (MQ=255)                                                                                                     gtcaggtcaCGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATGGGGTTAATTTTACGCGGCATGGGGGAAGAAc        >  1:198578/1‑133 (MQ=255)                                                                                                      tcaggtcaCGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCATGGTGGAAGAAcc       >  2:270285/1‑133 (MQ=255)                                                                                                          gtcaCGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCATGGTGGAAGAACCAAtc   >  2:341528/1‑133 (MQ=255)                                                                                                           tcaCGCTGTCAGCGGAAACCCGTCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCATGGTGGAAGAACCAAtct  <  1:186208/133‑1 (MQ=255)                                                                                                           tcaCGCTGCCAGCGGGAACCCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCATGGTGGAAGAACCAAtct  <  1:2813/133‑1 (MQ=255)                                                                                                                             |                                                                                                                   ACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACTTTATGCGGCATGGTGGAAGAACCAATCT  >  NC_000913/1190874‑1191112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑