BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,267,921	(A)_5→4	coding (93/987 nt)	yeiR →	zinc‑binding GTPase YeiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,917	0	A	.	99.5%	1836.4 / 5.7	436	coding (89/987 nt)	yeiR	zinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (245/186); total (248/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 488 total aligned reads displayed.

TTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGG  >  NC_000913/2267778‑2268052

tttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGGCCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGa                                                                                                                                         >  1:5059452/1‑140 (MQ=255)

    gCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaat                                                                                                                                     <  2:376374/140‑2 (MQ=255)

     ccAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAATCCACGTCGCTTCCTCATCTGTTAGCGCATAAAGATCCCAACGaaaatg                                                                                                                                    >  2:3254372/1‑138 (MQ=255)

       aGGATTAACGCTCAGTTAACTTACCAGGGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                  <  2:6588596/140‑5 (MQ=255)

       aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                  <  2:3998032/140‑5 (MQ=255)

       aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                  <  2:4539435/140‑5 (MQ=255)

       aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                  <  2:511409/140‑5 (MQ=255)

       aGGATTAACGCTCAGTCAACTTCCCAGAGTTATACTGAGTCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaatggg                                                                                                                                  <  2:3616533/140‑5 (MQ=255)

            tAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTa                                                                                                                            <  1:5467967/140‑1 (MQ=255)

             aaCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTAc                                                                                                                           <  1:5789020/140‑1 (MQ=255)

                   cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGTCCATAAAGATCTCAACG‑AAAAGGGGCGGTACTGGttc                                                                                                                     >  1:3242533/1‑139 (MQ=255)

                   cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTa                                                                                                                     <  2:4131937/140‑1 (MQ=255)

                    aGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTaa                                                                                                                    >  2:4999679/1‑140 (MQ=255)

                          cTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAAtt                                                                                                              >  1:488052/1‑140 (MQ=255)

                              ccAGAGTATTGCGGAGAAGTTGCGACCAGGACCGACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTggg                                                                                                          <  2:3467410/140‑1 (MQ=255)

                                agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCGAACG‑AAATTGGTCGGTACTGGTTAATGAATTTGGGGa                                                                                                        >  1:2640517/1‑140 (MQ=255)

                                agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                        >  1:1525337/1‑140 (MQ=255)

                                agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                        >  1:581661/1‑140 (MQ=255)

                                agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                        >  1:6228622/1‑140 (MQ=255)

                                agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGAGGa                                                                                                        >  1:6366117/1‑140 (MQ=255)

                                agagTATTGCGGAGCCGTTGTGACCAGGACCAACATCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                        >  1:7199323/1‑140 (MQ=255)

                                  agTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAg                                                                                                      >  1:4569070/1‑140 (MQ=255)

                                  agTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAg                                                                                                      <  1:740250/140‑1 (MQ=255)

                                    tATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTc                                                                                                    <  1:5452636/140‑1 (MQ=255)

                                    tATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGAGGAAGTc                                                                                                    <  2:6366117/140‑1 (MQ=255)

                                     aTTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCg                                                                                                   <  2:1431274/140‑1 (MQ=255)

                                       tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGTTACGGGTTAATGAGTTTGGGGAAGTCGGa                                                                                                 >  1:2983308/1‑140 (MQ=255)

                                       tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                 >  1:679729/1‑140 (MQ=255)

                                       tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                 >  1:248837/1‑140 (MQ=255)

                                       tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                 >  1:1021289/1‑140 (MQ=255)

                                       tGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGa                                                                                                 >  2:6094825/1‑140 (MQ=255)

                                         cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTggg                                                                                                          <  1:4018287/129‑1 (MQ=255)

                                         cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAAt                                                                                               <  2:5411501/140‑1 (MQ=255)

                                            aGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGa                                                                                                        >  1:1392255/1‑128 (MQ=255)

                                            aGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGa                                                                                            >  2:6239807/1‑140 (MQ=255)

                                                 ttGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGATACCGATTAATGAATTTGGGGAGGTCGGAATTGATGGTg                                                                                       >  2:3319364/1‑140 (MQ=255)

                                                    tgACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCtt                                                                                    <  2:488052/140‑1 (MQ=255)

                                                       ccAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGc                                                                                 <  2:1048145/140‑1 (MQ=255)

                                                        cAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGTGGTACTGGTTAACGAAATTGGGGAAGTCGGAATTGATGGTGCTTTGCt                                                                                >  2:3120350/1‑140 (MQ=255)

                                                        cAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGa                                                                                            <  2:5459317/128‑1 (MQ=255)

                                                           gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                                                                             >  1:74794/1‑140 (MQ=255)

                                                           gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                                                                             >  1:3774992/1‑140 (MQ=255)

                                                           gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                                                                             >  2:1224061/1‑140 (MQ=255)

                                                           gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                                                                             >  1:598392/1‑140 (MQ=255)

                                                           gACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGc                                                                             >  2:517059/1‑140 (MQ=255)

                                                                aCCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATa                                                                        >  1:1109523/1‑140 (MQ=255)

                                                                aCCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATa                                                                        >  1:6127566/1‑140 (MQ=255)

                                                                     atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                   >  1:6897744/1‑140 (MQ=255)

                                                                     atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggc                                                                   >  1:1267719/1‑140 (MQ=255)

                                                                     atcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAAAGAATTTGGGGAATTCGGAATTGATGTTGCTATGCTCGCCGATAgcggc                                                                   >  2:2322026/1‑140 (MQ=255)

                                                                      tcaCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAgcggcg                                                                  <  2:1539072/140‑1 (MQ=255)

                                                                       caCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGgcgc                                                                 <  2:598392/140‑1 (MQ=255)

                                                                        aCCGGTTTTCTCGGCTGCGGGATAATCACGTCGATTCGTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCa                                                                <  1:3121865/140‑1 (MQ=255)

                                                                        aCCGGTTTTCTCGGCAGTGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCa                                                                <  2:1871170/140‑1 (MQ=255)

                                                                        aCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCa                                                                <  2:7494777/140‑1 (MQ=255)

                                                                        aCCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCa                                                                <  2:4278734/140‑1 (MQ=255)

                                                                         ccGGTTTTCTCGGCATCGGGAATACAACGCCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCAt                                                               <  1:2053538/140‑1 (MQ=255)

                                                                         ccGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCAt                                                               <  2:5816335/140‑1 (MQ=255)

                                                                            gTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGc                                                            >  2:1614835/1‑140 (MQ=255)

                                                                            gTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGc                                                            >  1:7112057/1‑140 (MQ=255)

                                                                            gTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGAACTGGTTAATGAATTTGGGGAAGTCCGAATTGAGGGTGCTTTGCTCGCCGATAGCGGCGCATTGc                                                            >  2:3140466/1‑140 (MQ=255)

                                                                                   cGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGTGAAGTCGGAATTGTTGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAaga                                                     <  2:203564/140‑1 (MQ=255)

                                                                                      cAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGAt                                                  >  2:5349621/1‑140 (MQ=255)

                                                                                       aGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATc                                                 >  1:216008/1‑140 (MQ=255)

                                                                                         cGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATccc                                               >  2:4797484/1‑140 (MQ=255)

                                                                                         cGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATccc                                               >  1:4286071/1‑140 (MQ=255)

                                                                                              aaaCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCcggcg                                          <  2:679729/140‑1 (MQ=255)

                                                                                                     gTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCAt                                   >  1:6618745/1‑140 (MQ=255)

                                                                                                      tCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCAtg                                  >  1:1831693/1‑140 (MQ=255)

                                                                                                      tCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGCTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCAtg                                  >  2:1128068/1‑140 (MQ=255)

                                                                                                        gATTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCAtgtg                                <  1:1207122/140‑1 (MQ=255)

                                                                                                         aTTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgc                               >  1:5162137/1‑140 (MQ=255)

                                                                                                         aTTCTTCATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgc                               <  1:231542/140‑1 (MQ=255)

                                                                                                          ttcttcATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGGGCATTGCTGAACGAGATCCCCAGCGGCTGCATGtgct                              >  1:3484565/1‑140 (MQ=255)

                                                                                                           tcttcATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGtgctg                             >  1:4154784/1‑140 (MQ=255)

                                                                                                               cATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGtt                         <  2:7131513/140‑1 (MQ=255)

                                                                                                               cATCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGtt                         >  1:810546/1‑140 (MQ=255)

                                                                                                                agcTGTTAGCCCATAAAGATCCTAACG‑AAAATGGGCGATACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTa                        <  1:3360313/138‑1 (MQ=255)

                                                                                                                aTCTGTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTa                        <  2:1332968/140‑1 (MQ=255)

                                                                                                                    gTTAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTa                        >  1:5568676/1‑136 (MQ=255)

                                                                                                                     ttAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGt                   >  2:772605/1‑140 (MQ=255)

                                                                                                                     ttAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGt                   <  2:216008/140‑1 (MQ=255)

                                                                                                                      tAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGtt                  <  2:5558053/140‑1 (MQ=255)

                                                                                                                      tAGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGtt                  <  2:6945445/140‑1 (MQ=255)

                                                                                                                       aGCCCATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAACAGATCCCTGGCGGCAGCATGTGCTGCGTTAATGGttt                 >  1:2850606/1‑140 (MQ=255)

                                                                                                                         cccATAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTAc               <  2:673190/140‑1 (MQ=255)

                                                                                                                          ccATAAAGATCCCAACG‑GAAATGGGCGGTACGGGTAAATCAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTAcc              <  2:2983308/140‑1 (MQ=255)

                                                                                                                             tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCAt           >  1:6972971/1‑140 (MQ=255)

                                                                                                                             tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCAt           >  1:5316984/1‑140 (MQ=255)

                                                                                                                             tAAAGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCAt           <  1:167494/140‑1 (MQ=255)

                                                                                                                              aaaGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATg          <  1:4416142/140‑1 (MQ=255)

                                                                                                                              aaaGATCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCCGCGACTGCATGTACTGCGTTAATGGTTTACCCATg          >  2:2718182/1‑140 (MQ=255)

                                                                                                                                  aTCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAgg      <  2:5673831/140‑1 (MQ=255)

                                                                                                                                  aTCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAgg      <  1:6739718/140‑1 (MQ=255)

                                                                                                                                  aTCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAgg      <  2:4637417/140‑1 (MQ=255)

                                                                                                                                   tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGCGATCCCCGACGGGTGCGTGTGCTGCGTTAAAGGTTTACCCATGCAGGt     >  2:2633199/1‑140 (MQ=255)

                                                                                                                                   tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGt     >  1:5736039/1‑140 (MQ=255)

                                                                                                                                   tCCCAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGt     >  2:7385344/1‑140 (MQ=255)

                                                                                                                                    cccAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTa    <  1:6472623/140‑1 (MQ=255)

                                                                                                                                      cAACG‑AAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAgg  >  2:732265/1‑140 (MQ=255)

TTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTCGGAATTGATGGTGCTTTGCTCGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGG  >  NC_000913/2267778‑2268052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`