BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,910	(A)_6→5	coding (1213/1413 nt)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,905	0	A	.	99.8%	2076.7 / 0.2	487	coding (1208/1413 nt)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (252/232); total (253/234)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 514 total aligned reads displayed.

GCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGT  >  NC_000913/1756766‑1757040

   gTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACTTAAATACTGCCCGGATTCCACCATCTTGGCACTGACCACTAACGaaaa                                                                                                                                      >  2:3563638/1‑140 (MQ=255)

   gTATGCCGTGGTGCCATTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGaaaa                                                                                                                                      >  2:7121151/1‑140 (MQ=255)

    tATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGaaaaa                                                                                                                                     <  1:5194200/140‑1 (MQ=255)

           tggtgCCGTTGAAACTGCTGAATAACTGGTTGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             <  1:2321991/140‑1 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCATTGACCACCAACG‑AAAAACGGCtca                                                                                                                             <  2:7011051/140‑1 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             >  2:6918190/1‑140 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             <  1:4206027/140‑1 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             <  2:5918306/140‑1 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             >  2:5516572/1‑140 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             <  1:6687265/140‑1 (MQ=255)

           tggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             >  2:336623/1‑140 (MQ=255)

           tggtgCCGTTGAAACGGCTGAACAACTGGATGCTCCGTTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtca                                                                                                                             <  1:2595601/140‑1 (MQ=255)

             gtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtcatc                                                                                                                           >  2:446873/1‑140 (MQ=255)

              tgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtcatca                                                                                                                          >  2:727439/1‑140 (MQ=255)

              tgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCtcatca                                                                                                                          >  1:7174976/1‑140 (MQ=255)

                ccGTTGAAACTGCTGAATAACTGGATGCTCCGCTGGTCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGt                                                                                                                        <  2:3075502/140‑1 (MQ=255)

                ccGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGt                                                                                                                        <  2:1390528/140‑1 (MQ=255)

                       aaCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCGCCAACG‑AAAAACGGCTCATCAGTTGGTACt                                                                                                                 <  1:1270618/140‑1 (MQ=255)

                           gctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGc                                                                                                             >  2:6604221/1‑140 (MQ=255)

                             tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCaa                                                                                                           >  2:273130/1‑140 (MQ=255)

                             tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCaa                                                                                                           >  1:4892556/1‑140 (MQ=255)

                                          gCTCCGCTGATCGTGGTCGCTACTCAGGGCGCTAAATCTTCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCg                                                                                              <  2:2512638/140‑1 (MQ=255)

                                            tCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                            <  1:354344/140‑1 (MQ=255)

                                            tCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                            <  1:446873/140‑1 (MQ=255)

                                            tCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                            <  1:79991/140‑1 (MQ=255)

                                            tCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                            >  2:741475/1‑140 (MQ=255)

                                            tCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                            <  1:1411755/140‑1 (MQ=255)

                                            tCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                            <  1:6089291/140‑1 (MQ=255)

                                               gCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCt                                                                                         <  1:6632299/140‑1 (MQ=255)

                                                   aTCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGtt                                                                                     <  2:1859466/140‑1 (MQ=255)

                                                         gTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag                                                                               <  2:75800/140‑1 (MQ=255)

                                                         gTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag                                                                               <  1:5793552/140‑1 (MQ=255)

                                                         gTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag                                                                               <  1:5286059/140‑1 (MQ=255)

                                                           tGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAt                                                                             <  2:4190909/140‑1 (MQ=255)

                                                             cTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATGAGTTGGTAATGAACAAGGGCGTTGTGCCGCGGCTTGTTAAAGAGATCa                                                                           >  2:1960706/1‑140 (MQ=255)

                                                                   aGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTa                                                                     >  2:5207676/1‑140 (MQ=255)

                                                                    gggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTAc                                                                    >  1:5656695/1‑140 (MQ=255)

                                                                    gggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAAGCGTTGTGCCGTAGATTGTTAAAGAGATCACTTCTAc                                                                    >  1:2483797/1‑140 (MQ=255)

                                                                     ggCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACt                                                                   >  2:6456841/1‑140 (MQ=255)

                                                                      gCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtg                                                                  <  2:687227/140‑1 (MQ=255)

                                                                        ggTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgat                                                                >  2:5113298/1‑140 (MQ=255)

                                                                          tAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgat                                                                >  1:1584179/1‑138 (MQ=255)

                                                                          tAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtgat                                                                <  2:1584179/138‑1 (MQ=255)

                                                                          tAAATCTACTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCa                                                                           <  2:573980/127‑1 (MQ=255)

                                                                           aaaTCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTgatgat                                                             <  1:4718160/140‑1 (MQ=255)

                                                                                 gCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGAGTTGTGCCGCAGATTGGTAAAGAGACCACTTCTACTGATGATTTCTAc                                                       >  2:3147227/1‑140 (MQ=255)

                                                                                  cTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCGTGTCAAAGAGATGACTTGTACTGATGATTTCTAcc                                                      >  2:2010799/1‑140 (MQ=255)

                                                                                    cgcgCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGt                                                    <  1:6823233/140‑1 (MQ=255)

                                                                                    cgcgCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGt                                                    <  1:3791747/140‑1 (MQ=255)

                                                                                      cgcAGTACGTAAATACTTCCCGGATGCCTCCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCt                                                  >  1:7539850/1‑140 (MQ=255)

                                                                                      cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCt                                                  >  1:6335182/1‑140 (MQ=255)

                                                                                      cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCt                                                  <  1:5492606/140‑1 (MQ=255)

                                                                                      cgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCt                                                  <  2:6776483/140‑1 (MQ=255)

                                                                                       gcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTg                                                 >  2:1190226/1‑140 (MQ=255)

                                                                                                    aCTTCCCGGATGCCATCATCCTCGCCCTGCCCACCAATG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTgg                                    <  1:1932639/140‑1 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAGTTCTACTAATGATTTCAACCGACTGGGTAAAGAACTGGc                                   >  2:2225907/1‑140 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAGTTCAACTTATGATTTCCACCGTCTGGGTAAAGAACTGGc                                   >  2:2615743/1‑140 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGc                                   >  2:6150057/1‑140 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGc                                   >  1:7408847/1‑140 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATATCTACCGTCAGGGTAAAGAACTGGc                                   >  1:3162538/1‑140 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTGCTACTGAAGATGTCGACCGTCAGGGTAAAGAACTGGc                                   >  1:2193564/1‑140 (MQ=255)

                                                                                                     cTTCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAATTCGACTCATGATTTCAACCGTCTGGGTAAAGAACTGGc                                   >  2:3473581/1‑140 (MQ=255)

                                                                                                      ttCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAATGAGATCACATCTACTGCTGATTTCTACCGTCTGGGTAAAGAACTGGct                                  >  2:2927884/1‑140 (MQ=255)

                                                                                                       tcgcGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTa                                             <  1:5556136/125‑1 (MQ=255)

                                                                                                       tCCCGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctc                                 >  2:3755355/1‑140 (MQ=255)

                                                                                                        cccGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctct                                >  2:6533883/1‑140 (MQ=255)

                                                                                                        cccGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctct                                >  2:6371623/1‑140 (MQ=255)

                                                                                                        cccGGATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGAATTCTACCGTCTGGGTAAAGAACTGGctct                                >  1:3269846/1‑140 (MQ=255)

                                                                                                           ggATGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                             <  1:6533883/140‑1 (MQ=255)

                                                                                                             aTGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTATTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCaga                           >  2:4384798/1‑140 (MQ=255)

                                                                                                              tGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                          <  2:4979496/140‑1 (MQ=255)

                                                                                                              tGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                          <  2:1709634/140‑1 (MQ=255)

                                                                                                              tGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                          <  1:7332114/140‑1 (MQ=255)

                                                                                                              tGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                          <  1:7070190/140‑1 (MQ=255)

                                                                                                              tGCCACCATCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                          <  1:5426719/140‑1 (MQ=255)

                                                                                                                ccaccaTCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTg                               <  2:4068214/133‑1 (MQ=255)

                                                                                                                  accaTCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                      <  2:6104007/140‑1 (MQ=255)

                                                                                                                  accaTCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                      <  1:29393/140‑1 (MQ=255)

                                                                                                                   ccaTCCTGGCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTCCTGATGATTTCTCCTGTTTGGGTAAAGAACCAGCTCTGCAGAGCGGTc                     >  2:3671197/1‑140 (MQ=255)

                                                                                                                          ggCACTGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                          <  2:5442789/128‑1 (MQ=255)

                                                                                                                               tGACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAg         >  2:1089842/1‑140 (MQ=255)

                                                                                                                                gACCACCAACG‑AAAAACGGCTCATCAGTTTGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg        <  1:3671197/140‑1 (MQ=255)

                                                                                                                                gACCACCAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg        <  2:4623732/140‑1 (MQ=255)

                                                                                                                                gACCACCAACG‑AAAAACCGCTCATCAGTTGGCACTTAGCTAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg        <  2:2331586/140‑1 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  1:1825917/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  1:1702149/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  1:1002753/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  2:5346097/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  1:3927517/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  1:7247082/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       <  1:691798/140‑1 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  1:6427603/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCAGCTGAGCGGACTGGCACACAAAGGt       >  1:3493553/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCCGCAGAGCGGGCTGGCACACAAAGGt       >  1:2734025/1‑140 (MQ=255)

                                                                                                                                 accaccAACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTACCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt       >  2:5645445/1‑140 (MQ=255)

                                                                                                                                  ccaccaACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTg      <  1:502423/140‑1 (MQ=255)

                                                                                                                                   caccaACG‑AAAAACGGCTCATCCGTTTGTCCTGAGCAGAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGCACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGa     <  1:2030228/140‑1 (MQ=255)

                                                                                                                                      caACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTGTGCAGAGCGGTCTGGCACACAAAGGTGACGt  >  2:3186428/1‑140 (MQ=255)

                                                                                                                                      caACG‑AAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt  >  1:4611381/1‑140 (MQ=255)

                                                                                                                                      caACG‑AAAAACGGCTCATCAGTCGGAACTTAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt  <  1:2646207/140‑1 (MQ=255)

GCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGT  >  NC_000913/1756766‑1757040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`