BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,404	(C)_5→6	coding (579/1221 nt)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,399	1	.	C	97.0%	823.8 / 11.7	231	coding (584/1221 nt)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand): ref base . (0/7); new base C (63/161); total (63/168)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TT‑CC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTG  >  NC_000913/1831260‑1831532

gTAGTGCATATAGGCGTACAGTGCCACGGGGCGCCCGTCGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  1:3348943/140‑1 (MQ=255)

gTAGTGCATATAGGCATACAGTTCCCCGGTGGCCCCGACCCCGGTTTGGACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  2:3159016/140‑1 (MQ=255)

gTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  1:6711962/140‑1 (MQ=255)

gTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  1:748980/140‑1 (MQ=255)

gTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  2:6181026/140‑1 (MQ=255)

caggTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  1:7016873/137‑1 (MQ=255)

agacagCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑tt                                                                                                                                         <  1:5060975/135‑1 (MQ=255)

   gTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TT‑cc‑c                                                                                                                                    <  1:6839865/140‑1 (MQ=255)

   gTGCATATAGGCATACAGTTCCCCGCTGCACCCGACGTCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TT‑cc‑c                                                                                                                                    <  1:2102083/140‑1 (MQ=255)

          taGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TT‑cc‑cccctgga                                                                                                                             <  1:3948742/140‑6 (MQ=255)

           aGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTTCACAAATCACGCCGACCTTGTAAAAAAACCGTGCTGGCTGGGCCCACA‑CCCCC‑CT‑cc‑ccccccgat                                                                                                                            >  2:4462262/1‑134 (MQ=255)

           aGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGAt                                                                                                                             <  1:1000185/140‑1 (MQ=255)

           aGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGATACACAAATTACACAAACCCTTTAAAAAAACGTTGCTGGCTTGGCCCCCA‑ccccc‑cc‑cc‑ccccccccc                                                                                                                            >  2:1186823/1‑134 (MQ=255)

            ggCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATg                                                                                                                            <  1:6329757/140‑1 (MQ=255)

               cagcAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGt                                                                                                                         <  1:4686974/137‑1 (MQ=255)

               aTACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGt                                                                                                                         <  2:5608401/140‑1 (MQ=255)

                        cccGGTGCGCCCGACGCCGGTTTGCACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTCAAAACGCGCTGCTGGGTTGCGCCCCA‑CCCCCATC‑CC‑CCCCGGGTGGGTTCGGCAGTc                                                                                                                >  2:1880520/1‑140 (MQ=255)

                          cGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCAc                                                                                                              <  1:5815724/140‑1 (MQ=255)

                          cGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGCCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCAc                                                                                                              <  1:6949881/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAGTCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  2:3267727/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:5473373/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:4320317/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:880765/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:413004/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:4025978/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:1557194/140‑1 (MQ=255)

                            gTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCg                                                                                                            <  1:1293185/140‑1 (MQ=255)

                             tGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAAATCACCCAGACCCTGTAAAAAAACGTTGTTGGCTGGGGCCCCACCCCCC‑CC‑CC‑CCCCCGCTGGGGTCGACAAACAcccc                                                                                                           >  2:4366308/1‑138 (MQ=255)

                             tGCGCCCGACGCCGGTTTGTACGTCCTCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGc                                                                                                           <  1:2594019/140‑1 (MQ=255)

                               cgcCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGcac                                                                                                         <  1:16499/140‑1 (MQ=255)

                               cgcCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGcac                                                                                                         <  1:1182828/140‑1 (MQ=255)

                                    gACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTa                                                                                                    <  1:5096059/140‑1 (MQ=255)

                                     gcGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCCCGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACC‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTag                                                                                                   <  2:5240582/139‑1 (MQ=255)

                                     aCGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTag                                                                                                   <  1:4454677/140‑1 (MQ=255)

                                     aCGCCGGTTTGTACTTCATCAAAAAACAATAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTag                                                                                                   <  1:3261475/140‑1 (MQ=255)

                                       gCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTagag                                                                                                 <  2:7275058/140‑1 (MQ=255)

                                                    tcatcaAAAATCAACAGCGCATTGTCGCGCTTACACAGTTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAgcg                                                                                    <  1:2337476/140‑1 (MQ=255)

                                                      atcaAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAgcgcg                                                                                  <  1:5876605/140‑1 (MQ=255)

                                                      atcaAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAgcgcg                                                                                  <  1:4856987/140‑1 (MQ=255)

                                                          aaaaTCAACAGCGCAGTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTgg                                                                              <  1:3236494/140‑1 (MQ=255)

                                                           aaaTCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGc                                                                             <  2:3943067/140‑1 (MQ=255)

                                                           aaaTCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGACTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGc                                                                             <  1:4375320/140‑1 (MQ=255)

                                                           aaaTCAACAGCGCATTGTGGCGCTTCCACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGc                                                                             <  1:2935623/140‑1 (MQ=255)

                                                           aaaTCAACAGCGCATTGTGGCGCTTACACAACTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGc                                                                             <  2:3084349/140‑1 (MQ=255)

                                                             aTCAACAGCGCATTGTGGCGGTTACCCAACTCACGCATACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCag                                                                           <  1:1935028/140‑1 (MQ=255)

                                                                  cAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTa                                                                      <  1:5188168/140‑1 (MQ=255)

                                                                   aGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTaa                                                                     <  2:167464/140‑1 (MQ=255)

                                                                   aGCGCATTGTGGCGGCTACACACTTGACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTaa                                                                     <  1:3505415/140‑1 (MQ=255)

                                                                   aGCGCATTGTGGCGGATACACACTTGACGGAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTaa                                                                     <  1:3497201/140‑1 (MQ=255)

                                                                     cgcATTGTGGCGGTTACCCAATCCACGCAGACCTTTTAATAACGCGTTTCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  2:2113299/140‑1 (MQ=255)

                                                                     cgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  1:4817920/140‑1 (MQ=255)

                                                                     cgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  1:7215347/140‑1 (MQ=255)

                                                                     cgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  1:7347461/140‑1 (MQ=255)

                                                                     cgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  2:1111932/140‑1 (MQ=255)

                                                                     cgcATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  1:1373813/140‑1 (MQ=255)

                                                                     cacATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  2:7465911/138‑1 (MQ=255)

                                                                     acagttGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAata                                                                   <  1:4300408/136‑1 (MQ=255)

                                                                       cATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATc                                                                 <  1:1194529/140‑1 (MQ=255)

                                                                       cATTGTGGCGGTTACACAAATTACCCCGACCCTTTAAAAAAACGGTGCTGGGTGGCGCCCCACCCCCC‑CC‑CC‑CCCCCGGGGGGGGCGACAAACACCCCACACGGGGAGGCGTCAAACAACGCGCCGGCGGAGGTAaaatt                                                                 >  2:6028782/1‑139 (MQ=255)

                                                                          tgtgGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGtt                                                              <  1:6428630/140‑1 (MQ=255)

                                                                               cGGTTACACAATTCATGCAGACCTTATAACAACGCGTCGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATg                                                         <  1:3144821/140‑1 (MQ=255)

                                                                               cGGTTACACAATTCACGCAGACCTTGTAAAACCGCGTTGTTGGCTGGCTCCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATg                                                         <  2:3040266/140‑1 (MQ=255)

                                                                               cGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATg                                                         <  2:543646/140‑1 (MQ=255)

                                                                               aggTTACACAATTCACGTAGACCTTGTAAAACCGCCTTGTTGGCTGGCGCCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATg                                                         <  2:2714787/139‑1 (MQ=255)

                                                                                     cacaATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCAt                                                   <  1:15064/140‑1 (MQ=255)

                                                                                         aTTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCCGGATGGGTTCGACAATCACCGCCCAGGGAGGGTCGTCAATCAACGCTCTGGCAGAGGTACTATCGTTATATGCAGCATGACg                                               >  2:2815386/1‑140 (MQ=255)

                                                                                          ttCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGa                                              >  2:7363344/1‑140 (MQ=255)

                                                                                            cACGCAGACCTTGTAGAAACGCTTTCCTGCCTGGCACGACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAat                                            <  1:2035520/140‑1 (MQ=255)

                                                                                            cACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGATTTATTAGCGTTATATGCAGCATGACGact                                            >  1:2714787/1‑138 (MQ=255)

                                                                                            cACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAat                                            >  1:4985436/1‑140 (MQ=255)

                                                                                            cACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAat                                            >  2:1415235/1‑140 (MQ=255)

                                                                                               gCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATc                                         >  2:7052857/1‑140 (MQ=255)

                                                                                                  gACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATccgc                                      >  1:6752998/1‑140 (MQ=255)

                                                                                                   aCCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATccgcc                                     >  1:6561398/1‑140 (MQ=255)

                                                                                                   aCCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATccgcc                                     >  1:5586143/1‑140 (MQ=255)

                                                                                                         tAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGt                               >  1:3842663/1‑140 (MQ=255)

                                                                                                             aaCGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCACAGTTAATATCGTTAAACGCAGCATGACGAAAATCCGCCGGCAGTGgcg                           >  2:3381102/1‑140 (MQ=255)

                                                                                                             aaCGCGTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAAACACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGgcg                           >  1:4523496/1‑140 (MQ=255)

                                                                                                               cgcgTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCa                         <  2:2284544/140‑1 (MQ=255)

                                                                                                               cgcgTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCa                         <  1:552599/140‑1 (MQ=255)

                                                                                                               cgcgTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTCATATGCAGCATGACAAATATCCGCCGGCAGTGGCGCa                         >  1:3014858/1‑140 (MQ=255)

                                                                                                                 cgTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGAGGAATATCCGCCGGCAGTGGCGCaaa                       >  1:2815314/1‑140 (MQ=255)

                                                                                                                 cgTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCaaa                       <  2:6483376/140‑1 (MQ=255)

                                                                                                                 cgTTGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCaaa                       >  1:5724240/1‑140 (MQ=255)

                                                                                                                   ttGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAAt                     >  2:5845547/1‑140 (MQ=255)

                                                                                                                   ttGCTGGCTGGCACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAAt                     >  1:4474041/1‑140 (MQ=255)

                                                                                                                     gctggctgGCACCACG‑CCGCC‑TC‑CCTCCCCGGATGGGGTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATcc                   <  1:2282786/140‑1 (MQ=255)

                                                                                                                          ctggcACCACA‑CCGCT‑TTCCC‑CCCTGGATCGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGa              <  1:3596846/140‑1 (MQ=255)

                                                                                                                          ctggcACCACA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGa              >  1:3989226/1‑140 (MQ=255)

                                                                                                                              caccacC‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGGAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGaa             >  1:1071543/1‑137 (MQ=255)

                                                                                                                              caccacA‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAg          >  1:5961512/1‑140 (MQ=255)

                                                                                                                                 caca‑CCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggct       <  2:5070411/140‑1 (MQ=255)

                                                                                                                                  acagccGCC‑TTCCC‑CCCTGGATGGGTTCGACCATCTCCGCACAGGTAGTGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggct       <  2:2957627/136‑1 (MQ=255)

                                                                                                                                   ca‑cCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctgg     >  2:4862172/1‑140 (MQ=255)

                                                                                                                                   ca‑cCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctgg     >  2:2493229/1‑140 (MQ=255)

                                                                                                                                   ca‑cCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctgg     >  1:5246116/1‑140 (MQ=255)

                                                                                                                                   ca‑cCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctgg     >  1:3907813/1‑140 (MQ=255)

                                                                                                                                   ca‑cCGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATACCCGCCGGCAGTGGCACAAAATCCTGTGAATAggctgg     >  1:2970637/1‑140 (MQ=255)

                                                                                                                                       cGCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGgctggctg  <  2:3737429/140‑1 (MQ=255)

                                                                                                                                        gCC‑TTCCC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAggctgg     <  2:4582340/136‑1 (MQ=255)

GTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACA‑CCGCC‑TT‑CC‑CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTG  >  NC_000913/1831260‑1831532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`