BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,993	(T)_5→6	coding (752/1332 nt)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,988	1	.	T	96.3%	1358.2 / 31.1	403	coding (747/1332 nt)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand): ref base . (12/3); new base T (209/178); total (222/181)
Fisher's exact test for biased strand distribution p-value = 6.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

AAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949849‑2950122

aaaaGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTgcgc                                                                                                                                            >  1:1333385/1‑140 (MQ=255)

       gatTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑tt‑t‑t‑ttg                                                                                                                                 >  1:6012572/1‑138 (MQ=255)

            gTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTgg                                                                                                                             <  1:3954109/140‑1 (MQ=255)

               tttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGgcgc                                                                                                                          >  2:6896936/1‑140 (MQ=255)

                    tctTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGa                                                                                                                     <  2:5807689/140‑1 (MQ=255)

                           aGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTg                                                                                                              >  2:1101620/1‑140 (MQ=255)

                             agCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGcc                                                                                                            >  1:4631188/2‑140 (MQ=255)

                                gTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCa                                                                                                         >  1:1777958/1‑140 (MQ=255)

                                 tCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGCGCGC‑TT‑TCT‑TGCGTAGCGCAGAGAAAGCCTGCCGCAg                                                                                                        >  1:3402804/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCg                                                                                                           <  2:4788414/136‑1 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:473553/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:650932/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:6818849/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:1240390/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:3965814/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:142983/1‑140 (MQ=255)

                                  cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACACCGGTACGATGCCC‑TTAT‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:3154060/1‑140 (MQ=255)

                                    cTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgcgg                                                                                                     >  1:345073/1‑140 (MQ=255)

                                       aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt                                                                                                  >  1:6360827/1‑140 (MQ=255)

                                          acgacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcg                                                                                               >  1:6991757/1‑140 (MQ=255)

                                          acgacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcg                                                                                               <  2:3739226/140‑1 (MQ=255)

                                           cgacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcgt                                                                                              <  2:5232125/140‑1 (MQ=255)

                                             acgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                            <  2:831813/140‑1 (MQ=255)

                                             acgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                            >  2:671160/1‑140 (MQ=255)

                                               ggTGATATTGTCTCCGATCTTTGCCCTAACGGAGCTCAAACGCGGGTATAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  2:2495965/140‑1 (MQ=255)

                                               ggTGATATTGTCTCCGAGCTTTCCCCTAACGGAGCCCAAGCGCGGGTAAAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  2:2329755/140‑1 (MQ=255)

                                               ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          >  1:358665/1‑140 (MQ=255)

                                               ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:7544110/140‑1 (MQ=255)

                                               ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:6818849/140‑1 (MQ=255)

                                               ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCCCTTT‑T‑T‑TGGGTGGCGCAGTGAAAGCATGCCGCAGCGGCGTGCGTCGCt                                                                                          >  2:1916499/1‑140 (MQ=255)

                                               ggTGATATTGTCTCCGAACTTTACCATAAGGAAGCGCGAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:3064479/140‑1 (MQ=255)

                                                 tGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTATGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGt                                                                                        <  1:4766000/140‑1 (MQ=255)

                                                   atatTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGACACAGTGAAAGCCCGCCGCAGCGCCGTGCGTCGCTGTCa                                                                                      >  1:3092938/1‑140 (MQ=255)

                                                      ttGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTG‑AAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTa                                                                                  <  2:4053460/140‑1 (MQ=255)

                                                          ctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  1:3744710/139‑1 (MQ=255)

                                                           tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                                     >  2:5413158/1‑133 (MQ=255)

                                                           tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  1:6258307/138‑1 (MQ=255)

                                                           tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCa                                                                              >  2:117877/1‑140 (MQ=255)

                                                            ccGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAg                                                                             <  2:758159/140‑1 (MQ=255)

                                                            ccGAACTTTTCCATAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAg                                                                             <  1:4163793/140‑1 (MQ=255)

                                                             cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGTGCAGTGAAAGCCGGCCGCAGCGGCGGGCGTAGCTGTCATTTAATCAGt                                                                            >  2:2068701/1‑140 (MQ=255)

                                                             cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAGGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGt                                                                            >  2:2594887/1‑140 (MQ=255)

                                                             cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGt                                                                            >  2:6098228/1‑140 (MQ=255)

                                                              gAACTTTTCCCTAACGATGCGCGAGCGCGGGCAGATGCCCAGGAAGAGTAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  2:2662049/140‑1 (MQ=255)

                                                              gAACTTTTCCCTAACGATGCGCCAGCGCGGGTAGATGCCAAGGAAGAGCAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  2:2372140/140‑1 (MQ=255)

                                                              gAACTTTTCCCTAACGAGGCGCCAGCGCGGGCAGAGGCCAAGGAAGAGTAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  2:2795405/140‑1 (MQ=255)

                                                              gAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  2:4848245/140‑1 (MQ=255)

                                                              gAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  1:1128481/140‑1 (MQ=255)

                                                              gAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  1:4446957/140‑1 (MQ=255)

                                                              gAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt                                                                           <  2:3318155/140‑1 (MQ=255)

                                                                aCTTTTCCCTAACGAGGCGCAATCGCGGGTAGAAGCCGAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTAt                                                                         <  1:2796693/140‑1 (MQ=255)

                                                                aCTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTACCGCAGCGGCGGGCATCGCTGGCATTTAATCAGTTAt                                                                         >  1:2325704/1‑140 (MQ=255)

                                                                    ttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  2:322972/129‑1 (MQ=255)

                                                                    ttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGAAGCGGCGGGCGACGCTGTCATTTAATCAGTTATCAgg                                                                     >  1:2372140/1‑140 (MQ=255)

                                                                             gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGtcg                                                            <  2:7528328/140‑1 (MQ=255)

                                                                             gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            <  1:671160/140‑1 (MQ=255)

                                                                             gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            <  1:6098228/140‑1 (MQ=255)

                                                                             gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            <  1:7096523/140‑1 (MQ=255)

                                                                              aagcgcaagtgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTAC‑ACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg                                                          >  1:7446933/1‑140 (MQ=255)

                                                                               agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg                                                          >  2:6163769/1‑140 (MQ=255)

                                                                               agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg                                                          <  1:4866492/140‑1 (MQ=255)

                                                                                gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  1:4014804/140‑1 (MQ=255)

                                                                                 cgcaagcgcGGGTAGAGGCCCAAGAAGAGAAGGGCGATTTCAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        <  2:3402804/140‑1 (MQ=255)

                                                                                 cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        >  1:4939107/1‑140 (MQ=255)

                                                                                   caagcgcGGGTAGAAGCCCAGGCAGAGAATGGCGATTCCAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGt                                                      <  1:2416041/140‑1 (MQ=255)

                                                                                   caagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGt                                                      <  1:660848/140‑1 (MQ=255)

                                                                                     agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAGTCAGTTATCAGGAAGATGGCGCGCTGTTg                                                    >  1:2031760/1‑140 (MQ=255)

                                                                                      gcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCACGCTGTTGc                                                   <  1:833083/140‑1 (MQ=255)

                                                                                        gcgGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCaa                                                 <  1:7208301/140‑1 (MQ=255)

                                                                                           ggTAGAAGCCCAGGAAGAGAAATGCTATTACAACTCCCGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag                                              <  1:2640808/140‑1 (MQ=255)

                                                                                           ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  1:1005495/129‑1 (MQ=255)

                                                                                           ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         >  2:1005495/1‑129 (MQ=255)

                                                                                           ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag                                              >  2:4266572/1‑140 (MQ=255)

                                                                                                 aGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTtc                                        <  1:5067445/140‑1 (MQ=255)

                                                                                                 aGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTtc                                        >  2:1310691/1‑140 (MQ=255)

                                                                                                      aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcg                                   <  1:7341518/140‑1 (MQ=255)

                                                                                                      aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCACAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcg                                   >  2:7319884/1‑140 (MQ=255)

                                                                                                       ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  <  2:6300897/140‑1 (MQ=255)

                                                                                                       ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  >  2:1600924/1‑140 (MQ=255)

                                                                                                       ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  <  2:7430985/140‑1 (MQ=255)

                                                                                                         aaGAGAAAGGCGATTGCAACTCCGGCACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  1:3203191/140‑1 (MQ=255)

                                                                                                         aaGAGAAAGGCGATTACAACTCCGGTTCGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  2:5782646/140‑1 (MQ=255)

                                                                                                           gagaAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACg                              >  1:5194243/1‑140 (MQ=255)

                                                                                                                  gCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCgg                       <  2:4939107/140‑1 (MQ=255)

                                                                                                                  gCGATTACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCgg                       >  1:5673736/1‑140 (MQ=255)

                                                                                                                      ttACAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTa                           >  1:5813097/1‑132 (MQ=255)

                                                                                                                        aCAACTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCa                 >  2:6003998/1‑140 (MQ=255)

                                                                                                                          aaCTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  2:4631188/140‑1 (MQ=255)

                                                                                                                          aaCTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  1:4357807/140‑1 (MQ=255)

                                                                                                                          aaCTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  2:3787354/140‑1 (MQ=255)

                                                                                                                          aaCTCCGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  1:650932/140‑1 (MQ=255)

                                                                                                                               cGGTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATtgtg          <  2:1240390/140‑1 (MQ=255)

                                                                                                                               cGGTACGGTGCGC‑TT‑T‑TCTGCGTGGCGCAGTTAAAACCTACCGCAGCGTCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATtgtg          <  2:2207332/140‑1 (MQ=255)

                                                                                                                                 gTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGAt        >  2:7074661/1‑140 (MQ=255)

                                                                                                                                 gTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACAGTATCGGTACGCAGATTGTGAt        >  2:3059742/1‑140 (MQ=255)

                                                                                                                                 gTACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCGGTTGCAAGAGTTGTTCTTACGCGACCGTATCGGTACGCACATTGTGAt        >  2:3574475/1‑140 (MQ=255)

                                                                                                                                  tACGGTGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATg       >  2:5106220/1‑140 (MQ=255)

                                                                                                                                       tGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:147367/1‑140 (MQ=255)

                                                                                                                                       tGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  2:7169590/1‑140 (MQ=255)

                                                                                                                                       tGCGCTTT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:5439602/1‑140 (MQ=255)

AAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TT‑T‑T‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949849‑2950122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`