BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	94.5%	1091.9 / 42.3	310	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (12/5); new base C (115/177); total (128/182)
Fisher's exact test for biased strand distribution p-value = 1.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 348 total aligned reads displayed.

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTT  >  NC_000913/3815741‑3816014

cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                                                                                                                           <  1:6736437/140‑1 (MQ=255)

cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                                                                                                                           <  1:380111/140‑1 (MQ=255)

    aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c‑cc                                                                                                                                    <  1:4427198/140‑2 (MQ=255)

     atcTGTCGCTGATATGGTTTCTTGCCTTCGCTCCTCATCTTGCTTTTCTACAGAC‑AAAAAAAAGGCGACTCAGCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c‑cct                                                                                                                                   <  2:7524372/138‑3 (MQ=255)

     aaCTGGCGCTGATATGGATTCATGCCTTCGCCCCTTATCGTACTTTTCCACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c‑cct                                                                                                                                   <  2:2280687/140‑3 (MQ=255)

          gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGc                                                                                                                               <  1:4207739/140‑1 (MQ=255)

           cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGc                                                                                                                               >  1:6813124/1‑140 (MQ=255)

           cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGcc                                                                                                                              >  2:4799269/1‑140 (MQ=255)

           cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGcc                                                                                                                              >  1:5646335/1‑140 (MQ=255)

             cTGATATGGTTTCATGCTTTCGATCCTCATCTTACTTTTATACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCag                                                                                                                            <  2:2939065/140‑1 (MQ=255)

             cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCag                                                                                                                            <  1:4750679/140‑1 (MQ=255)

               gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                          <  2:551390/140‑1 (MQ=255)

               gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                          <  2:7187446/140‑1 (MQ=255)

               gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCACCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                          >  2:2831066/1‑140 (MQ=255)

                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CTC‑C‑CTCGGGCCAGAGc                                                                                                                         >  1:2982213/1‑140 (MQ=255)

                 tatGGTTTCATGCCTTCGCTCCCCATCTTACTTTTATACTGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGcc                                                                                                                        <  2:2502110/140‑1 (MQ=255)

                 tatGGTTTCATGCCTTCCCTCCCCATCTTACATTTATACCGAC‑AAAAACAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGcc                                                                                                                        <  2:1984102/140‑1 (MQ=255)

                    ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTTATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCcaac                                                                                                                     <  1:1766011/140‑1 (MQ=255)

                      tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCcaacaa                                                                                                                   <  1:5616332/140‑1 (MQ=255)

                       ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAg                                                                                                                  <  1:911859/140‑1 (MQ=255)

                        tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGa                                                                                                                 <  1:6025068/140‑1 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCca                                                                                                                       >  2:4859952/1‑132 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATg                                                                                                               >  2:440404/1‑140 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATg                                                                                                               >  1:1856945/1‑140 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGAGTCGCTACAATGGAATCGATTCC‑C‑C‑CTCGGTCCAGAGCCAACAAGATg                                                                                                               >  2:3349942/1‑140 (MQ=255)

                           tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAGT‑C‑CTC‑CGCGGGCCGGAGCCAACAAGATGa                                                                                                              >  1:2061687/1‑140 (MQ=255)

                            gCCTTCGCTCCTCATCCGACTTTTCTACAGAG‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAg                                                                                                             <  2:3261196/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGCTTCTATTCC‑C‑C‑CTCGGGCCAGGGCCAACAAGATGAGTAg                                                                                                          >  2:2621707/1‑140 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  1:7349906/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          >  2:4727397/1‑140 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  2:5747471/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  1:1263236/140‑1 (MQ=255)

                                 cGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGct                                                                                                        >  1:1558442/1‑140 (MQ=255)

                                      ctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CAC‑C‑CTCGGGCCATAGTCAACAACATGAGTAGCTCTTCa                                                                                                   >  1:3532027/1‑140 (MQ=255)

                                        cATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                 >  2:4996265/1‑140 (MQ=255)

                                        cATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑CACTCGGGCCAGATCCGACAAGAGGAGTAGCTCTTCATg                                                                                                 >  1:2865277/1‑140 (MQ=255)

                                             tACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGa                                                                                            <  2:3999814/140‑1 (MQ=255)

                                              aCTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                                                                           >  2:887764/1‑140 (MQ=255)

                                              aCTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                                                                           >  1:1460140/1‑140 (MQ=255)

                                              aCTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                                                                           >  1:4848016/1‑140 (MQ=255)

                                                ttttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                                                                                   >  2:5629320/1‑130 (MQ=255)

                                                  ttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                       >  1:4051556/1‑140 (MQ=255)

                                                  ttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                       <  2:1720732/140‑1 (MQ=255)

                                                   tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                      >  2:308701/1‑140 (MQ=255)

                                                   tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGACt                                                                                      >  1:6682208/1‑140 (MQ=255)

                                                    cTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCAAGCATGATGAGTAGCTCACCATGGGTGAACGGCTc                                                                                     >  2:3554523/1‑140 (MQ=255)

                                                     tACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                    >  2:3839351/1‑140 (MQ=255)

                                                       cAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                  <  2:5093257/140‑1 (MQ=255)

                                                       cAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                  <  2:6528511/140‑1 (MQ=255)

                                                       cAGAC‑AAAAAAAAGGCGACTCAGCATTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                  <  1:3276595/140‑1 (MQ=255)

                                                          aC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                               >  1:4346662/1‑140 (MQ=255)

                                                             aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                             <  2:4579816/140‑1 (MQ=255)

                                                             aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                             >  1:7575257/1‑140 (MQ=255)

                                                               aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                           >  2:6386148/1‑140 (MQ=255)

                                                               aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                           <  2:5302572/140‑1 (MQ=255)

                                                               aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGACGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                           <  2:7319870/140‑1 (MQ=255)

                                                                  aaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                        <  1:6507739/140‑1 (MQ=255)

                                                                    aGGCGACTCATCAGTCGCCTTACAAATCATTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCGACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                      <  1:3659200/140‑1 (MQ=255)

                                                                     ggCGACTCCTCAGTCGTCTTAAGAATCAGTTCGCCAGCGCCGCCTTCTTCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                     <  2:3516685/140‑1 (MQ=255)

                                                                     ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                     <  2:6311659/140‑1 (MQ=255)

                                                                     ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                     >  2:7029299/1‑140 (MQ=255)

                                                                     ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                     >  1:1176606/1‑140 (MQ=255)

                                                                     ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                        >  1:4637133/1‑137 (MQ=255)

                                                                       cGACTCATGAGTCGCCTTAAAACTCAGTTTGACGGAGCCGCCTTCTGCCTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:3650517/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCGTTAAAACTCTGTTTGCCAGCGGCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  1:3165043/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:3729186/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:4346662/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:1558442/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  1:3946350/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:1163322/140‑1 (MQ=255)

                                                                       cGACTCATCAGTCGCCTCAAAATTCAGTTTGCCAGCGCCCCCTTCTGCCTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:1949041/140‑1 (MQ=255)

                                                                         aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                      >  2:6866639/1‑135 (MQ=255)

                                                                         aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                                 >  2:4048011/1‑140 (MQ=255)

                                                                         aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                                 <  2:1301837/140‑1 (MQ=255)

                                                                         aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCACTTAATGGGTGAACGGATCGCCTTCTGCCGTCCCCTGCa                                                                 >  2:3055551/1‑140 (MQ=255)

                                                                             atcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc                                                             >  1:267080/1‑140 (MQ=255)

                                                                                 gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   >  2:969415/1‑130 (MQ=255)

                                                                                   cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgat                                                       <  2:267080/140‑1 (MQ=255)

                                                                                     ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                     >  1:3880161/1‑140 (MQ=255)

                                                                                     ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                     >  1:5480915/1‑140 (MQ=255)

                                                                                       ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                   <  2:1856342/140‑1 (MQ=255)

                                                                                           aaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGACTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGt                                               <  2:6682208/140‑1 (MQ=255)

                                                                                             aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                             <  1:6283508/140‑1 (MQ=255)

                                                                                              tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                            <  1:5140587/140‑1 (MQ=255)

                                                                                                 gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                         <  2:1451329/140‑1 (MQ=255)

                                                                                                   ttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                       <  1:1036595/140‑1 (MQ=255)

                                                                                                     gCCAGCGCCGCCTTCCGCGTCGCTACAATTGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                     <  1:2271421/140‑1 (MQ=255)

                                                                                                       cAGCGCCGCCTTCTGCGCCGCTCCAATGGATGCGAAT‑C‑CTC‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                   <  2:2449799/140‑1 (MQ=255)

                                                                                                        aGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                  <  2:6781754/140‑1 (MQ=255)

                                                                                                          cgccgcCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTGAATGATGCGCCCGGCTTCGGTCATCACTa                                >  1:3412198/1‑140 (MQ=255)

                                                                                                          cgccgcCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTa                                >  1:991017/1‑140 (MQ=255)

                                                                                                             cgccTTCTGCGTCGCGACAATGTATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                             <  1:2621707/140‑1 (MQ=255)

                                                                                                               ccTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                           <  1:5387710/140‑1 (MQ=255)

                                                                                                                     gCGTCGCTACAATGGATACGATTCC‑C‑C‑CTCGTGCCCGAGCCAACAAGACGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                     <  2:2076100/140‑1 (MQ=255)

                                                                                                                       gTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                   <  2:377328/140‑1 (MQ=255)

                                                                                                                         cGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct                 >  1:7193373/1‑140 (MQ=255)

                                                                                                                              cAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg            <  1:7029299/140‑1 (MQ=255)

                                                                                                                                  ggATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag        <  2:5972257/140‑1 (MQ=255)

                                                                                                                                   gattcgattcC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga       >  1:6415867/1‑140 (MQ=255)

                                                                                                                                    attcgattcC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCagag      >  1:5027647/1‑140 (MQ=255)

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTT  >  NC_000913/3815741‑3816014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`