BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,693	C→T	P9L (CCG→CTG)	yegQ →	putative peptidase YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,693	0	C	T	99.4%	1160.4 / NA	334	P9L (CCG→CTG)	yegQ	putative peptidase YegQ
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (185/147); minor base G (1/1); total (186/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

TTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGA  >  NC_000913/2165559‑2165828

  aCCAGAGCCCCCCACAGCGCTACAATGCCCGTCCTAAAACTGGGGGCATTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGa                                                                                                                                  <  2:2108168/140‑1 (MQ=255)

  aCCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGa                                                                                                                                  <  1:7133311/140‑1 (MQ=255)

   ccAGAGCCCCCCACACCGCTACAATGCCCCCCCTTAACGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGaa                                                                                                                                 <  1:2877826/140‑1 (MQ=255)

    cAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAAc                                                                                                                                <  1:6273257/140‑1 (MQ=255)

      gagCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGc                                                                                                                              <  2:1758210/140‑1 (MQ=255)

        gCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTg                                                                                                                            <  1:6811331/140‑1 (MQ=255)

         ccccccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGa                                                                                                                           >  1:3258899/1‑140 (MQ=255)

         ccccccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGa                                                                                                                           >  1:7572205/1‑140 (MQ=255)

              cacaGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAat                                                                                                                      >  2:4823678/1‑140 (MQ=255)

                   cgcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGTAACTCCTATCCCTGCCGGGAAGGCTGAAAAATATGCg                                                                                                                 >  1:2726725/1‑140 (MQ=255)

                   cgcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCg                                                                                                                 <  1:5035072/140‑1 (MQ=255)

                    gcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGc                                                                                                                              >  2:1041446/1‑126 (MQ=255)

                     cTACAATGACCGCCCTTAAAGTTGGGGCACTCCCCAAACAGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGtt                                                                                                               <  2:3372306/140‑1 (MQ=255)

                      tACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTa                                                                                                              <  1:566005/140‑1 (MQ=255)

                          aTGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGATAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGCAACGCTGAAAAATATGCGTTACGCt                                                                                                          <  2:5151299/140‑1 (MQ=255)

                          aTGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCt                                                                                                          <  1:6475227/140‑1 (MQ=255)

                             cccgcccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTc                                                                                                       >  1:3921298/1‑140 (MQ=255)

                             cccgcccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTc                                                                                                       >  1:530970/1‑140 (MQ=255)

                             cccgcccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTc                                                                                                       >  2:4159890/1‑140 (MQ=255)

                                gcccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCt                                                                                                    >  2:764120/1‑140 (MQ=255)

                                  ccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTa                                                                                                  >  1:4673721/1‑140 (MQ=255)

                                     tAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATgg                                                                                               >  2:4349103/1‑140 (MQ=255)

                                       aaGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGgcg                                                                                             <  1:5794724/140‑1 (MQ=255)

                                       aaGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACACTTTCGCTTATGgcg                                                                                             >  1:1099928/1‑140 (MQ=255)

                                       aaGTGGGGGCACTCCCCAAACCGCTTCATCACGTGGAGCCGATCTGACTTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGgcg                                                                                             <  2:2656387/140‑1 (MQ=255)

                                           gggggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGa                                                                                         <  2:1547267/140‑1 (MQ=255)

                                            ggggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGAt                                                                                        >  2:4387952/1‑140 (MQ=255)

                                            ggggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGAt                                                                                        >  1:4901821/1‑140 (MQ=255)

                                             gggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAATGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATg                                                                                       <  1:1591472/140‑1 (MQ=255)

                                             gggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATg                                                                                       <  1:5137073/140‑1 (MQ=255)

                                              ggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATg                                                                                       <  2:520261/139‑1 (MQ=255)

                                                        tAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCg                                                                            >  2:559585/1‑140 (MQ=255)

                                                         aaCCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCgg                                                                           >  1:4499690/1‑140 (MQ=255)

                                                            cGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGcc                                                                        >  1:1613783/1‑140 (MQ=255)

                                                              cTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAg                                                                      <  2:6489162/140‑1 (MQ=255)

                                                              cTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAg                                                                      <  2:6988687/140‑1 (MQ=255)

                                                              cTTCATCAGGTGAAGCGCATCTAACCTGTCATCAGTACGTGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAg                                                                      <  2:2356819/140‑1 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCt                                                                                     <  1:1519516/124‑1 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAg                                                                      >  1:1263240/1‑139 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  1:1123464/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  1:218990/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  2:3891277/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  2:1350963/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  2:4576287/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  1:6236090/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  2:5508241/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  1:3368007/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     <  2:6444141/140‑1 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  1:4334940/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCCTTCGCTTTTGGAGCAGATGCTTTTTATGCGGGCCAGc                                                                     >  1:2963151/1‑140 (MQ=255)

                                                               ttCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACACTTTCGCGTATGGCGCGGATGCTGTTTATGCGGGCCAGc                                                                     >  2:2953384/1‑140 (MQ=255)

                                                               ttCATCAGGGGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGc                                                                     >  2:7114051/1‑140 (MQ=255)

                                                                    caGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGt                                                                <  1:4387952/140‑1 (MQ=255)

                                                                      ggTGAAGCGGATCTGACTTGTCATCAGAACGCGAGTATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTa                                                              <  2:2277511/140‑1 (MQ=255)

                                                                      ggTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTGATGGCGCAGATCCTGTTTATGAGGGCCAGCCGCGTTa                                                              >  1:2382329/1‑140 (MQ=255)

                                                                         gAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTc                                                           <  2:7378681/140‑1 (MQ=255)

                                                                           aGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTccc                                                         >  2:1497283/1‑140 (MQ=255)

                                                                            gCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCt                                                        >  1:6601880/1‑140 (MQ=255)

                                                                             cGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTTGCTTATGGAGCAAATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTg                                                       >  2:2478159/1‑140 (MQ=255)

                                                                              ggATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGc                                                      <  2:572796/140‑1 (MQ=255)

                                                                              ggATCTGACCTGTCATCAGAACCAGCGAAATATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGc                                                      <  1:2114028/140‑1 (MQ=255)

                                                                                          tcatcaGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTAtt                                                            <  1:3845337/122‑1 (MQ=255)

                                                                                          tcatcaGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGcaaca                                          >  1:1859751/1‑140 (MQ=255)

                                                                                          tcatcaGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCACGATATTCCATGCGTGTGCGcaaca                                          >  1:2576283/1‑140 (MQ=255)

                                                                                               aGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCAGGCCAGCGGCGTTATTTCCTGGGTGTGCGCAACAACGaa                                     >  2:1973632/1‑140 (MQ=255)

                                                                                                  aCGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTc                                  <  1:6844379/140‑1 (MQ=255)

                                                                                                    gagagaATTATGTTTAAACCGGAACTCCTTTGCCTGGCGGGAACGCTGAAAAATATGCTTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCaa                                >  1:6105778/1‑140 (MQ=255)

                                                                                                    gagagaATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCCGCCCCGTTATTCCCTGAGTGTCCGCAACAACGAATTCaa                                >  2:2849304/1‑140 (MQ=255)

                                                                                                    gagagaATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCaa                                >  1:1428293/1‑140 (MQ=255)

                                                                                                    gagagaATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCaa                                >  2:1116536/1‑140 (MQ=255)

                                                                                                       agaATTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCa                             <  1:7090547/140‑1 (MQ=255)

                                                                                                         aaTTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACg                           >  1:7336322/1‑140 (MQ=255)

                                                                                                          aTTATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGa                          <  1:228315/140‑1 (MQ=255)

                                                                                                           ttATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGaa                         >  2:7219811/1‑140 (MQ=255)

                                                                                                           ttATGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGaa                         >  1:4449502/1‑140 (MQ=255)

                                                                                                             aTGTTTAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGaaaa                       >  2:6780140/1‑140 (MQ=255)

                                                                                                                tttAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTTCCTCCGGATGCGCAACAACGATTTCAACCACGAAAATCt                    >  2:3299970/1‑140 (MQ=255)

                                                                                                                tttAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCt                    >  1:6518888/1‑140 (MQ=255)

                                                                                                                  tAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGCAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTc                  >  1:7400596/1‑140 (MQ=255)

                                                                                                                  tAAACCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTc                  >  2:6101370/1‑140 (MQ=255)

                                                                                                                   aaaCCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGTGTGTGCGTAACTACCAATTCATCCACGAAAATCTTCa                 >  1:3176306/1‑140 (MQ=255)

                                                                                                                   aaaCCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGGGTGTGCGTAACGACGAATTCAGCCACGAAAATCTTCa                 >  1:1924245/1‑140 (MQ=255)

                                                                                                                   aaaCCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCa                 >  2:5371348/1‑140 (MQ=255)

                                                                                                                   aaaCCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCa                 >  2:3777832/1‑140 (MQ=255)

                                                                                                                     aCCGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTCATTCCCTGCGTGAGCGTAACAACGATTTCACCCACGAAAATCTTCAGc               >  2:2317469/1‑140 (MQ=255)

                                                                                                                      ccGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCt              <  2:4934458/140‑1 (MQ=255)

                                                                                                                      ccGGAACTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCt              <  2:4449502/140‑1 (MQ=255)

                                                                                                                      ccGGAACTCCTTTCCCTCGCGGCAACGCTGATAAACATGCGTTACGCTGTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCt              <  2:2176028/140‑1 (MQ=255)

                                                                                                                          aaCTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCGACAACGAATTCACCCACGAAAATCTTCAGCTCGGc          >  2:2580367/1‑140 (MQ=255)

                                                                                                                          aaCTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGc          >  1:419265/1‑140 (MQ=255)

                                                                                                                           aCTCCTTTCCCTGGCGGGAACGTTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCa         <  1:3650852/140‑1 (MQ=255)

                                                                                                                           aCTCCTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCa         <  1:5422707/140‑1 (MQ=255)

                                                                                                                           aCTCCTTTACCTGGCGTGAACGGTGAAAAAATTACTTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCa         <  2:3602810/140‑1 (MQ=255)

                                                                                                                               cTTTCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCaa     >  2:183920/1‑140 (MQ=255)

                                                                                                                                 ttCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATg   >  1:5867191/1‑140 (MQ=255)

                                                                                                                                 ttCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATg   >  2:7315217/1‑140 (MQ=255)

                                                                                                                                  tCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGa  <  1:4553227/140‑1 (MQ=255)

                                                                                                                                  tCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGa  <  2:318020/140‑1 (MQ=255)

                                                                                                                                  tCCCTGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGa  <  1:1005576/140‑1 (MQ=255)

                                                                                                                                   ccctGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCa         >  2:5840332/1‑132 (MQ=255)

TTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGA  >  NC_000913/2165559‑2165828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`