BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,577,589	A→G	C263C (TGT→TGC)	maeB ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,577,589	0	A	G	97.5%	961.1 / 3.2	277	C263C (TGT→TGC)	maeB	malate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (125/144); total (129/148)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

Only 100 of 285 total aligned reads displayed.

GTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCA  >  NC_000913/2577452‑2577723

gTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATCTCCAGGGTCAGCACTTTCGGGCCGGAGCa                                                                                                                                      >  1:3320550/1‑140 (MQ=255)

  gCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGagcagc                                                                                                                                    >  1:6223801/1‑140 (MQ=255)

    aaaTGATGGCATCCGTACGCACTTCGTTCACCAGCGGTGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGccc                                                                                                                                  <  1:3103411/140‑1 (MQ=255)

      atgatgGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAg                                                                                                                                >  2:5160561/1‑140 (MQ=255)

           ggCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGaaaa                                                                                                                           >  1:5294928/1‑140 (MQ=255)

               tCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGTTCAGCACTTTCTGGACGGAGCAGCCCAGGAAAATATc                                                                                                                       >  1:2428991/1‑140 (MQ=255)

                ccGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATcc                                                                                                                      <  1:298429/140‑1 (MQ=255)

                 cGGACGCACTTCTTTCGGCAGCGGCGGCAGAATTTCCGGCTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCcg                                                                                                                     <  2:2872548/140‑1 (MQ=255)

                 cGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCcg                                                                                                                     <  2:5083935/140‑1 (MQ=255)

                 cGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCcg                                                                                                                     <  1:212333/140‑1 (MQ=255)

                   gACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCcgcg                                                                                                                   <  1:6763515/140‑1 (MQ=255)

                   gACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCcgcg                                                                                                                   <  2:1800805/140‑1 (MQ=255)

                   gACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCcgcg                                                                                                                   <  1:5940253/140‑1 (MQ=255)

                    cagCACTTCTTTCGCCAGCGGCGGCTGAAGTTCCGGTGCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCgcgc                                                                                                                  <  1:2358821/138‑1 (MQ=255)

                      gCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCt                                                                                                                <  1:1010669/140‑1 (MQ=255)

                       cACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCtt                                                                                                               >  2:5596139/1‑140 (MQ=255)

                          ttcttTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCaa                                                                                                            <  1:5854627/140‑1 (MQ=255)

                          ttcttTCGCCAGCGGAGGCAGAGTTACCGTTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCaa                                                                                                            <  1:3505860/140‑1 (MQ=255)

                            cttTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATc                                                                                                          <  2:4082760/140‑1 (MQ=255)

                             tttCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATca                                                                                                         >  1:6526065/1‑140 (MQ=255)

                              ttCGCCAGCGGCGGCCGAATTTTCGCTTCGGGGTTCGTCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATcac                                                                                                        <  1:2837264/140‑1 (MQ=255)

                              ttCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATcac                                                                                                        <  1:195167/140‑1 (MQ=255)

                              ttCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATcac                                                                                                        <  1:7258971/140‑1 (MQ=255)

                               tCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATcaca                                                                                                       >  2:4577824/1‑140 (MQ=255)

                                 gCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCAcatc                                                                                                     >  1:1553742/1‑140 (MQ=255)

                                    aGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACatcatc                                                                                                  >  1:7167073/1‑140 (MQ=255)

                                     gcggcgGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCg                                                                                                 <  2:584615/140‑1 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATcac                                                                                                        >  2:1390970/1‑132 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACatcatc                                                                                                  <  2:6700183/138‑1 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGa                                                                                                >  2:1054073/1‑140 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGa                                                                                                >  1:754046/1‑140 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGa                                                                                                >  2:1607846/1‑140 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGa                                                                                                >  2:1749284/1‑140 (MQ=255)

                                      cggcggCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCAGGCCGGAGGAGCCCAGAAAAATATCCGCGCGTTCAATCACATCATCGa                                                                                                >  2:2450462/1‑140 (MQ=255)

                                      cggcgaCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCaa                                                                                                            <  1:7307201/128‑1 (MQ=255)

                                       ggcggcAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAg                                                                                               <  1:5596139/140‑1 (MQ=255)

                                       ggcggcAGAATTTCCGGTTCCGGGTCCGCCAGCGCCATGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAg                                                                                               <  1:2775468/140‑1 (MQ=255)

                                         cggcAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAggg                                                                                             <  2:5301189/140‑1 (MQ=255)

                                               aaTTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCGGCGCCTTCCATCAGATCATCGAGGGTACGtt                                                                                       >  2:3160420/1‑140 (MQ=255)

                                                  ttccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGc                                                                                    <  1:894379/140‑1 (MQ=255)

                                                  ttccggttccggGTTCGCCAGCACCAGGATCGTTGCCGCTCGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGc                                                                                    <  2:3265790/140‑1 (MQ=255)

                                                    ccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCg                                                                                  <  1:1712461/140‑1 (MQ=255)

                                                    ccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCg                                                                                  <  1:100862/140‑1 (MQ=255)

                                                         tccggGTTCGCCAGCGGCAGGATCATTGGCGGAGGCGCCTTTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATc                                                                             <  2:3387697/140‑1 (MQ=255)

                                                         tccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATc                                                                             <  1:4824777/140‑1 (MQ=255)

                                                         tccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATc                                                                             >  1:1329954/1‑140 (MQ=255)

                                                          ccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATcc                                                                            <  2:7167073/140‑1 (MQ=255)

                                                           cggGTTCGCCAGCGCCAGGATCATTCGCGCACGAGCCGTTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATcca                                                                           <  1:2630200/140‑1 (MQ=255)

                                                            gggTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATccac                                                                          <  2:791431/140‑1 (MQ=255)

                                                               ttCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCcaccac                                                                       <  1:6106616/140‑1 (MQ=255)

                                                                 cgccagcgccagGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCg                                                                     >  2:1491654/1‑140 (MQ=255)

                                                                  gccagcgccagGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGc                                                                    <  1:4906959/140‑1 (MQ=255)

                                                                  gccagcgccagGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAACCACTTCATCGAGGGTAGGTTTGCCGTCATCCACCACCGc                                                                    >  2:2630200/1‑140 (MQ=255)

                                                                      gcgccagGATCATTGGCGCACGAGCCATTTTATTCCCCATTTCCTGGGACAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCatat                                                                <  2:2673190/140‑1 (MQ=255)

                                                                       cgccagGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATg                                                               <  2:6037109/140‑1 (MQ=255)

                                                                                cATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTCgg                                                      <  2:4821423/140‑1 (MQ=255)

                                                                                 aTTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGt                                                     <  2:4397184/140‑1 (MQ=255)

                                                                                  ttGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCACAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGtt                                                    >  1:4122727/1‑140 (MQ=255)

                                                                                       gcACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGc                                               >  2:592743/1‑140 (MQ=255)

                                                                                        cACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGcc                                              >  2:4488904/1‑140 (MQ=255)

                                                                                        cACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGcc                                              <  1:3783009/140‑1 (MQ=255)

                                                                                                   ttcttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTc                                   <  1:31984/140‑1 (MQ=255)

                                                                                                   ttcttcACCATTTCCCGGGTCATCAATTTCGGGCCGGTGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTc                                   <  1:2253325/140‑1 (MQ=255)

                                                                                                    tcttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                  <  1:4503392/140‑1 (MQ=255)

                                                                                                    tcttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                  <  1:5680815/140‑1 (MQ=255)

                                                                                                    tcttcACCATTTCCTGGGTCAGAACTTTCTGGCCGGACCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                  <  1:2450462/140‑1 (MQ=255)

                                                                                                      ttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACg                                <  2:7339504/140‑1 (MQ=255)

                                                                                                      ttcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACg                                >  1:6063241/1‑140 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCGGCACTTTCGGGCCTGAGCAGCACAGGAAAACATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                               <  2:2048780/140‑1 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCGGCACTTTCGGGCCGGAGCAGCACAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCGCCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                               <  2:2428215/140‑1 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                               <  2:3688/140‑1 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                               <  2:6417151/140‑1 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                               <  2:1234484/140‑1 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACgg                               >  1:4249375/1‑140 (MQ=255)

                                                                                                       tcACCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCCTTGGTTTCCGCCATGTTTGGCTCCCgg                               <  2:4220614/140‑1 (MQ=255)

                                                                                                         aCCATTTCCTGGGTCAGTACTTTCGGGCCGGTGCAGCCCCGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGcc                             <  2:3015221/140‑1 (MQ=255)

                                                                                                         aCCATTTCCTGGGTCAGGACTTTCGGGCCGGGGCAGCCCAGGAAAATAGCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGcc                             <  2:3081296/140‑1 (MQ=255)

                                                                                                         aCCATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGcc                             <  1:5021912/140‑1 (MQ=255)

                                                                                                          ccATTTCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGccc                            <  1:694478/140‑1 (MQ=255)

                                                                                                             tttCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTga                         >  2:4743155/1‑140 (MQ=255)

                                                                                                              ttCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTgat                        <  1:4488904/140‑1 (MQ=255)

                                                                                                              ttCCTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTgat                        >  1:4592230/1‑140 (MQ=255)

                                                                                                                ccTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTgatag                      >  1:1187970/1‑140 (MQ=255)

                                                                                                                ccTGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCACCATGTTTGGCTCACGGCCCt                           >  1:4760627/1‑135 (MQ=255)

                                                                                                                  tGGGTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTgatagat                    >  2:7007126/1‑140 (MQ=255)

                                                                                                                     gTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCa                                  >  2:6555909/1‑123 (MQ=255)

                                                                                                                     gTCAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTAGGTTTCCTCCATGTTTAGCTCACGGCCCTGATAGATAAc                 >  2:2260172/1‑140 (MQ=255)

                                                                                                                       cAGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGc               >  2:395754/1‑140 (MQ=255)

                                                                                                                        aGCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGcc              >  1:1808854/1‑140 (MQ=255)

                                                                                                                         gCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCt             >  2:877258/1‑140 (MQ=255)

                                                                                                                         gCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCt             >  2:6891087/1‑140 (MQ=255)

                                                                                                                         gCACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCt             >  2:5665748/1‑140 (MQ=255)

                                                                                                                         gCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAAc                 <  1:737834/136‑1 (MQ=255)

                                                                                                                         gCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAAc                 >  2:728016/1‑136 (MQ=255)

                                                                                                                          cACTTTCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGAACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCtt            >  1:5208782/1‑140 (MQ=255)

                                                                                                                              ttCGGGCCGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGa        <  2:5564743/140‑1 (MQ=255)

                                                                                                                                    ccGGAGCAGCCCAGGCAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCa  <  1:2043872/140‑1 (MQ=255)

                                                                                                                                    ccGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCa  >  2:6223231/1‑140 (MQ=255)

                                                                                                                                    ccGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCa  <  1:395754/140‑1 (MQ=255)

                                                                                                                                    ccGGAGCAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGCTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCa  >  2:5127407/1‑140 (MQ=255)

GTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCA  >  NC_000913/2577452‑2577723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`