BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,649,354	G→A	N978N (AAC→AAT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,649,354	0	G	A	99.4%	1136.7 / ‑5.2	333	N978N (AAC→AAT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (159/170); total (161/172)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

TCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGAC  >  NC_000913/2649217‑2649490

  agCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGTCAAACGGCAGTGTAACGGAGCGTTCGCCCTGTTCATTGa                                                                                                                                      >  2:2521990/1‑140 (MQ=255)

  agCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGa                                                                                                                                      >  2:5735690/1‑140 (MQ=255)

   gCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAg                                                                                                                                     >  2:4320954/1‑140 (MQ=255)

    cAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGc                                                                                                                                    >  1:1785134/1‑140 (MQ=255)

    cAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGc                                                                                                                                    >  1:5079310/1‑140 (MQ=255)

         aCCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCCGTGTAACCGAGCCTCCGCCCTATTCATTGAGCGTTAc                                                                                                                               >  1:2956846/1‑140 (MQ=255)

             gTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGc                                                                                                                           <  2:3850292/140‑1 (MQ=255)

                cGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAgc                                                                                                                        >  1:7294819/1‑140 (MQ=255)

                   cAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGTGCCTTCGACCTATTGATTGAGAGTTACCGGCAGCGcc                                                                                                                     >  1:3115349/1‑140 (MQ=255)

                   cAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGcc                                                                                                                     <  2:6222585/140‑1 (MQ=255)

                   cAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGcc                                                                                                                     >  1:5196784/1‑140 (MQ=255)

                        aTCACTTTACTTTCGTTGCTACCGAAGTCATATGCTGTCAAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCctgctg                                                                                                                <  2:3115349/140‑1 (MQ=255)

                           actttactttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCAc                                                                                                             <  2:80461/140‑1 (MQ=255)

                               tactttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACa                                                                                                         >  2:6504594/1‑140 (MQ=255)

                                actttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACaa                                                                                                        >  2:6440616/1‑140 (MQ=255)

                                 ctttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAat                                                                                                       >  1:5647859/1‑140 (MQ=255)

                                 ctttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAat                                                                                                       >  1:891737/1‑140 (MQ=255)

                                 ctttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAat                                                                                                       <  2:1434489/140‑1 (MQ=255)

                                 ctttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCG‑CTGCTGCACGACAata                                                                                                      >  1:5938297/1‑140 (MQ=255)

                                     cGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTg                                                                                                   >  2:5131814/1‑140 (MQ=255)

                                     cGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCACCAGGACGCGCACTTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTg                                                                                                   <  2:3030371/140‑1 (MQ=255)

                                      gTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGa                                                                                                  >  2:199220/1‑140 (MQ=255)

                                       ttGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGAc                                                                                                 >  1:4663839/1‑140 (MQ=255)

                                                 aGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGAcc                                                                                       <  1:1410657/140‑1 (MQ=255)

                                                   tCATCTGCCGTCGAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACcgg                                                                                     <  1:1005150/140‑1 (MQ=255)

                                                   tCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACcgg                                                                                     <  2:1013702/140‑1 (MQ=255)

                                                   tCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACcgg                                                                                     <  2:522192/140‑1 (MQ=255)

                                                       cTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGt                                                                                 >  2:6642497/1‑140 (MQ=255)

                                                           cGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac                                                                             <  2:5940212/140‑1 (MQ=255)

                                                              ccAGGCTTGCGCCATATCGCGCGATTCACCGTTAAAAACGCCAATCGGCAGTGTAGCCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccac                                                                          <  1:2776542/140‑1 (MQ=255)

                                                               cAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTaccacc                                                                         <  1:1526683/140‑1 (MQ=255)

                                                                   cTTGCGCCATGACGCGCCATTCTCCGTTAAACTCGTCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGt                                                                     <  2:2374163/140‑1 (MQ=255)

                                                                      gcgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTc                                                                  <  1:4320954/140‑1 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATTTTGACGTGATTGACGGGCGGTGTACCACCACGTTTCa                                                                 >  1:2300852/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCa                                                                 >  1:6939572/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCa                                                                 >  2:4443254/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCa                                                                 >  2:15419/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCa                                                                 >  1:4936195/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGGTTACCTCCACGTTTCa                                                                 >  2:7227025/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATGGACATGATTGACCGGCGGGTTACCACCACGTTTCa                                                                 >  2:3364394/1‑140 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCAGCACGACAGTATCGACGTGATTGACGGGCGGGTTACCACCACGatttc                                                                 >  2:3003790/1‑138 (MQ=255)

                                                                       cgcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCAGCACGACACTATAGACGTGATTGACTGGCGGTTTACCACCACGTTTCa                                                                 >  2:2429023/1‑140 (MQ=255)

                                                                        gcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAg                                                                <  1:7046202/140‑1 (MQ=255)

                                                                        gcCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAg                                                                <  1:1707074/140‑1 (MQ=255)

                                                                            tGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCtca                                                            <  2:1611746/140‑1 (MQ=255)

                                                                                    aaTTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGGCCGGCGGGTTACCAACACGTTTCAGCTCATCACCATc                                                    >  1:3280684/1‑140 (MQ=255)

                                                                                        cACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGGCTTCGCCCTGTTTATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCa                                                <  2:2608578/140‑1 (MQ=255)

                                                                                          ccGTTAAAATCGCCACTCGGCACTGCAACGGAGCCTTTGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCAcc                                              <  1:3549840/140‑1 (MQ=255)

                                                                                          ccGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCAcc                                              >  2:4380577/1‑140 (MQ=255)

                                                                                              tAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGaaa                                          >  2:564308/1‑140 (MQ=255)

                                                                                              tAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGaaa                                          >  2:6741993/1‑140 (MQ=255)

                                                                                              tAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTAACCGGCGGATTACCACAACGTTTTAGCTCATAACCCTCCTCCCCGaaa                                          >  2:3587171/1‑140 (MQ=255)

                                                                                                aaaTCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACg                                        >  1:7110243/1‑140 (MQ=255)

                                                                                                  aTCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCa                                      <  2:1184114/140‑1 (MQ=255)

                                                                                                  aTCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCa                                      <  1:7372118/140‑1 (MQ=255)

                                                                                                  aTCGCCAATCGGCAGTGTAACCAAGCCTTAGCCCTGTCCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCa                                      <  1:2463508/140‑1 (MQ=255)

                                                                                                    cGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCaga                                    <  2:7110243/140‑1 (MQ=255)

                                                                                                     gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCagag                                   >  1:605069/1‑140 (MQ=255)

                                                                                                     gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCagag                                   <  2:4936195/140‑1 (MQ=255)

                                                                                                     gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAACTCGTCGCCATCGACACCGAAACGCagag                                   >  1:2134558/1‑140 (MQ=255)

                                                                                                     gCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCCGTTTACCATCACCTATAGGCTCATCTCCATAGCCACCGAAACGCagag                                   >  2:3674857/1‑140 (MQ=255)

                                                                                                       cAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTAACACCCCGATTCCACCCGTCACCATCGCCCCCGAAACGCAGAGCt                                 >  2:489224/1‑140 (MQ=255)

                                                                                                           cGGCAGTGTAACCGAGCCTTCGCCCTGTTCAGTGAGCGTTACCGGCAGTGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCa                             <  2:2290846/140‑1 (MQ=255)

                                                                                                             gCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGa                           <  2:5623084/140‑1 (MQ=255)

                                                                                                             gCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGa                           <  1:6598310/140‑1 (MQ=255)

                                                                                                             gCAGTGTAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCACCTGCTGCACGACAATTTTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGa                           <  2:1736349/140‑1 (MQ=255)

                                                                                                                   tAACCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGcccc                     >  1:6160161/1‑140 (MQ=255)

                                                                                                                    aaCCGAGCCTTCGCCCTGTTCAATGCGCGATACCGGCCGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCt                    <  1:3342751/140‑1 (MQ=255)

                                                                                                                     aCCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCCCCGAAAAGCAGAGTTGCCAGACGCCCCTg                   >  1:2926381/1‑140 (MQ=255)

                                                                                                                     aCCGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTg                   >  1:6317789/1‑140 (MQ=255)

                                                                                                                      ccGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGa                  >  1:1261799/1‑140 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTGATTGAGCGTTACCCGCAGCGCGTGCTGCACAACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  2:3280684/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGTGCGTTACCTGCATCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  2:2995268/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGGGCGTTACCAGCATCGCGTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  2:2926381/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGGGCCTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  1:3011999/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGCGCGTTACCAGCACCGCTTGCTGCACTACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  2:2830942/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCTCCTGAc                 <  2:1785134/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  1:1573190/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  2:6280466/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  2:605069/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGCTCATTGGGCGTTAGCGGCAGCTCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  1:2521990/140‑1 (MQ=255)

                                                                                                                       cGAGCCTTCGCCCTGCTCATTGCGCATTAGCGGCAGCTCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAc                 <  1:2421898/140‑1 (MQ=255)

                                                                                                                        gAGCCTTCGCCCTGTTCCTTGACCGTTACCGCCAGCGCCCGCTGCACGCCAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAcc                <  2:1970005/140‑1 (MQ=255)

                                                                                                                        gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAcc                >  1:5351416/1‑140 (MQ=255)

                                                                                                                        gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAcc                <  2:5519418/140‑1 (MQ=255)

                                                                                                                        gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAcc                >  1:5711957/1‑140 (MQ=255)

                                                                                                                        gAGCCTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGAcc                >  2:5057886/1‑140 (MQ=255)

                                                                                                                           ccTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTc             <  2:7068169/140‑1 (MQ=255)

                                                                                                                           ccTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTc             >  2:4093884/1‑140 (MQ=255)

                                                                                                                           ccTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTc             >  1:113963/1‑140 (MQ=255)

                                                                                                                           ccTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGGTTACAACCACGTTTCGGCTCCACACCATCGCCACCGGAACGCAGAGCCGCCAAACCCCCCTGCCCCTc             >  2:6771420/1‑140 (MQ=255)

                                                                                                                           ccTTCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATCGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTc             >  1:6574880/1‑140 (MQ=255)

                                                                                                                             ttCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCaa           >  1:5600230/1‑140 (MQ=255)

                                                                                                                             ttCGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCaa           >  2:4412216/1‑140 (MQ=255)

                                                                                                                               cGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATa         >  1:439377/1‑140 (MQ=255)

                                                                                                                               cGCCCTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCCAAACGCAGAGCTGCCAGAACCCCCCGACCTTCAATa         >  2:4617074/1‑140 (MQ=255)

                                                                                                                                  ccTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAAcc      <  2:7356455/140‑1 (MQ=255)

                                                                                                                                   cTGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCt     <  1:3888954/140‑1 (MQ=255)

                                                                                                                                    tGTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTg    <  2:4115251/140‑1 (MQ=255)

                                                                                                                                     gTTCATTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACATTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGa   >  1:4426737/1‑140 (MQ=255)

TCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGAC  >  NC_000913/2649217‑2649490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`