Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,720,083 T→C V44A (GTA→GCA)  patZ → peptidyl‑lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,720,0830TC99.8% 1345.6 / ‑5.7 399V44A (GTA→GCA) patZpeptidyl‑lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (174/224);  total (175/224)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 430 total aligned reads displayed.

GACCGGTATGAGTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCT  >  NC_000913/2719946‑2720218
                                                                                                                                         |                                                                                                                                       
gaCCGGTATGAGTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCAc                                                                                                                                       >  2:5124276/1‑140 (MQ=255)
 aCCGGTATGAGTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACt                                                                                                                                      <  1:6096247/140‑1 (MQ=255)
        tGAGTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAAACTGTTGGGGGGAGGCTTTAACGGACCGGCACTCCCgggg                                                                                                                               >  2:2271624/1‑138 (MQ=255)
         gAGTCAGCGAGGACTTGAAGCACTAGTGCGACCAAAAGCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGa                                                                                                                              <  1:2650149/140‑1 (MQ=255)
           gTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGTGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACg                                                                                                                            >  1:7235885/1‑140 (MQ=255)
           gTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACg                                                                                                                            >  1:5913568/1‑140 (MQ=255)
               gCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTGACCTGCTGGCGGAAAGACTTAACGGCCCGGCACTCCCGGGGACGCCAg                                                                                                                        >  2:3670491/1‑140 (MQ=255)
               gCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAg                                                                                                                        >  2:1748114/1‑140 (MQ=255)
                cGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGTGGGTGGCGTTAACGGAACGGCACTCCCGGTGACGCCAGc                                                                                                                       >  2:2200722/1‑140 (MQ=255)
                cGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGc                                                                                                                       >  2:6569737/1‑140 (MQ=255)
                cGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGc                                                                                                                       >  2:153616/1‑140 (MQ=255)
                cGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGc                                                                                                                       >  2:811767/1‑140 (MQ=255)
                   ggACTGGAAGCACTACTTCGACCAAAATCGATAGCCGTAATTGGCGCGGCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTg                                                                                                                    <  2:2690255/140‑1 (MQ=255)
                   ggACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTg                                                                                                                    <  1:1532092/140‑1 (MQ=255)
                        ggAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGGGACGCCCACCCGGaaaa                                                                                                               >  2:6496206/1‑139 (MQ=255)
                        ggAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCATTATCGGCCCGGCACTGCCGGCGACGCCAGCCTGGAAAg                                                                                                               >  2:2821793/1‑140 (MQ=255)
                        ggAAGCACTACTGCGACC‑AAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGc                                                                                                              >  1:19213/1‑140 (MQ=255)
                         gAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCTCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGc                                                                                                              <  2:2424098/140‑1 (MQ=255)
                         gAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGc                                                                                                              <  2:4578485/140‑1 (MQ=255)
                             cACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGgtg                                                                                                          >  2:3929528/1‑140 (MQ=255)
                              actactGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGgtgt                                                                                                         >  1:4870448/1‑140 (MQ=255)
                               ctactGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGtt                                                                                                        >  1:4245703/1‑140 (MQ=255)
                                 actGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTgg                                                                                                      <  2:5037670/140‑1 (MQ=255)
                                 actGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTgg                                                                                                      >  1:4196387/1‑140 (MQ=255)
                                     cGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGgtgt                                                                                                  >  1:6924408/1‑140 (MQ=255)
                                      gACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGgtgtg                                                                                                 <  2:3808032/140‑1 (MQ=255)
                                       aCCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCCGCCTGGAAAGCGGGGTTGGgggtgt                                                                                                >  1:351795/1‑140 (MQ=255)
                                       aCCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGgt                                                                                                           >  1:5869835/1‑129 (MQ=255)
                                       aCCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTgg                                                                                                      <  1:4985890/134‑1 (MQ=255)
                                       aCCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGgtgtgt                                                                                                >  1:7462596/1‑140 (MQ=255)
                                       aCCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGACTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCgg                                                                                                            >  2:6978627/1‑128 (MQ=255)
                                           aaaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTtggc                                                                                            <  1:1584971/140‑1 (MQ=255)
                                            aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTtggcc                                                                                           >  1:6738005/1‑140 (MQ=255)
                                                gATAGCGGTAATTGGTGCGTCGATGGAACTCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTtggcctggc                                                                                       <  1:2953295/140‑1 (MQ=255)
                                                   aGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCgg                                                                                    <  2:1275010/140‑1 (MQ=255)
                                                    gCGGTAATTGGCGCGTTGATGAAACCCAATCGCTCGGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGa                                                                                   <  2:3567454/140‑1 (MQ=255)
                                                    gCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGa                                                                                   <  2:5205316/140‑1 (MQ=255)
                                                      ggTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGata                                                                                 >  1:4775105/1‑140 (MQ=255)
                                                       gTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGatat                                                                                >  2:4981738/1‑140 (MQ=255)
                                                        tAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATAtt                                                                               >  1:7390702/1‑140 (MQ=255)
                                                              gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGc                                                                         <  1:3860291/140‑1 (MQ=255)
                                                               cgcgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCt                                                                        <  1:1748114/140‑1 (MQ=255)
                                                               cgcgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCt                                                                        >  1:1010992/1‑140 (MQ=255)
                                                                 cgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTg                                                                      <  2:304985/140‑1 (MQ=255)
                                                                 cgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTg                                                                      <  2:6738005/140‑1 (MQ=255)
                                                                 cgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTg                                                                      <  2:7096493/140‑1 (MQ=255)
                                                                 cgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTg                                                                      >  2:927210/1‑140 (MQ=255)
                                                                  gTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGGGTTGGCCTGGCCGGAAATTGCCAGCTTGc                                                                     >  1:6441569/1‑140 (MQ=255)
                                                                    cGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGccc                                                                   >  2:5697293/1‑140 (MQ=255)
                                                                      aTGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTG‑CAGCTTGCCCttt                                                                >  1:3971755/1‑140 (MQ=255)
                                                                            cccAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACAcc                                                           <  1:652801/140‑1 (MQ=255)
                                                                                aTCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGGCTGGCCGAATAATGGCAGCTTGCCCTTTACACCCGAc                                                       >  1:3643304/1‑140 (MQ=255)
                                                                                aTCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGa                                                        <  2:7315328/139‑1 (MQ=255)
                                                                                aTCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGAc                                                       >  1:5085874/1‑140 (MQ=255)
                                                                                 tCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACAcc                                                           >  2:4012238/1‑135 (MQ=255)
                                                                                   gcgcAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCtt                                                    <  2:19213/140‑1 (MQ=255)
                                                                                    cgcAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTg                                                   >  2:5602414/1‑140 (MQ=255)
                                                                                      cAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                 <  1:5602414/140‑1 (MQ=255)
                                                                                      cAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                 <  2:6924408/140‑1 (MQ=255)
                                                                                      cAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                 <  1:7011434/140‑1 (MQ=255)
                                                                                       aGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCgg                                                <  1:5707574/140‑1 (MQ=255)
                                                                                       aGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCgg                                                <  2:6635089/140‑1 (MQ=255)
                                                                                        ggTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGt                                               <  2:5874254/140‑1 (MQ=255)
                                                                                         gTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGtt                                              <  2:6185393/140‑1 (MQ=255)
                                                                                           tACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGtttt                                            >  1:7162551/1‑140 (MQ=255)
                                                                                             ccTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTAt                                          <  1:6600905/140‑1 (MQ=255)
                                                                                             ccTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTAt                                          >  2:424416/1‑140 (MQ=255)
                                                                                             ccTGATGATGCGTAACCTGCTGCCGCGAGACTTTAACTGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTAt                                          <  1:2354742/140‑1 (MQ=255)
                                                                                                   gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGAcc                                                      <  1:228957/122‑1 (MQ=255)
                                                                                                   gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCtt                                                    >  2:5269330/1‑124 (MQ=255)
                                                                                                   gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCa                                    >  2:6064485/1‑140 (MQ=255)
                                                                                                   gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTAGCCTTTTACACCTGACCGTGCGGTTTTATGTACCa                                    >  2:2479314/1‑140 (MQ=255)
                                                                                                     tgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAAt                                  <  1:5187775/140‑1 (MQ=255)
                                                                                                     tgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGGGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGAGATTGCCAGCTTGCCCTTTACACCCGGCCTTGCGGGTTTATGTACCAAt                                  >  2:3181860/1‑140 (MQ=255)
                                                                                                      gCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATg                                 <  1:5363100/140‑1 (MQ=255)
                                                                                                       cGTAACCTGCTGGCGGGAGGCTTTAACGGCCCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGc                                <  2:4506244/140‑1 (MQ=255)
                                                                                                       cGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGc                                <  2:4146060/140‑1 (MQ=255)
                                                                                                       cGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGc                                <  1:767454/140‑1 (MQ=255)
                                                                                                       cGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGc                                <  2:1078937/140‑1 (MQ=255)
                                                                                                        gTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGcc                               <  1:211367/140‑1 (MQ=255)
                                                                                                        gTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGcc                               <  1:3755850/140‑1 (MQ=255)
                                                                                                        gTAACCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACAACCGACCTTGCGGTCTTATGTACCAATGcc                               >  1:3042786/1‑140 (MQ=255)
                                                                                                           aCCTGCTGGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGc                            >  2:1046200/1‑140 (MQ=255)
                                                                                                             ctgctgGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCg                          <  1:1079730/140‑1 (MQ=255)
                                                                                                             ctgctgGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCg                          <  1:4035732/140‑1 (MQ=255)
                                                                                                                ctgGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCCGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTaa                       >  1:1214135/1‑140 (MQ=255)
                                                                                                                ctgGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGcc                               <  2:5793546/132‑1 (MQ=255)
                                                                                                                ctgGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTaa                       >  1:933220/1‑140 (MQ=255)
                                                                                                                 tgGCGGGAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAAt                      >  1:46348/1‑140 (MQ=255)
                                                                                                                    cGGGAGGCTGTTTCGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCtt                   <  2:4701908/140‑1 (MQ=255)
                                                                                                                      ggAGGCTTTAACGGACCTGCACCCCCGGTGAGGCCAGCCAGGAAAGCGTTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGc                 <  2:2062480/140‑1 (MQ=255)
                                                                                                                      ggAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGc                 <  2:293445/140‑1 (MQ=255)
                                                                                                                      ggAGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGc                 <  2:720082/140‑1 (MQ=255)
                                                                                                                        aGGCTTTAACGGACCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGctc               <  1:1345889/140‑1 (MQ=255)
                                                                                                                         ggCTTTAACGGACCGTCACTCCGGGCGACGCCAGCCTCGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGctct              <  1:3181860/140‑1 (MQ=255)
                                                                                                                         ggCTTTAACGGACCGGCCCTCCCGGTGACGCCAGCCTGGAAAGCGGTGGTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGctct              <  2:3396744/140‑1 (MQ=255)
                                                                                                                         ggCTTTAACGGACCGGCACTCCCGGTGACACCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGctct              <  1:4403250/140‑1 (MQ=255)
                                                                                                                          gcgttAACGGACCGGCACTCCCGGGGACGCCAGCCTGGAAAGCGGTGGTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACTCCCGACCTAGCGGTATTCTGTACCAATTCCAGCCGTAATGTTGCtctt             >  2:3588061/4‑140 (MQ=255)
                                                                                                                                    aCCGGCACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGc   >  2:4802537/1‑140 (MQ=255)
                                                                                                                                     ccGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCt  >  2:7530995/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GACCGGTATGAGTCAGCGAGGACTGGAAGCACTACTGCGACCAAAATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCT  >  NC_000913/2719946‑2720218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑