BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,718	G→A	C146C (TGC→TGT)	thyA ←	thymidylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,718	0	G	A	99.5%	1846.9 / NA	548	C146C (TGC→TGT)	thyA	thymidylate synthase
Reads supporting (aligned to +/- strand): ref base G (2/0); major base A (279/262); minor base C (0/3); total (283/265)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 559 total aligned reads displayed.

CACTGCTGCGCCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGGCACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGG  >  NC_000913/2964581‑2964852

cACTGCTGCGCCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACa                                                                                                                                      >  1:5012789/1‑140 (MQ=255)

cACTGCTGCGCCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACa                                                                                                                                      >  1:6276338/1‑140 (MQ=255)

 aCTGCTGCGCCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGacac                                                                                                                                     <  1:3846276/140‑1 (MQ=255)

   tgctgcGCCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACgg                                                                                                                                   >  1:3956204/1‑140 (MQ=255)

        cgcCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGCGTTTGCCGACTGTCACGTAGAACTGAAAGAGTGCATGACACGGTGCCa                                                                                                                              >  2:2883744/1‑140 (MQ=255)

        cgcCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCa                                                                                                                              >  2:1055399/1‑140 (MQ=255)

        cgcCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCa                                                                                                                              >  1:5495034/1‑140 (MQ=255)

           catcatATGCACCAATAACGCGTAGCTGGGAATGTTGCACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAgcg                                                                                                                           <  1:3206688/140‑1 (MQ=255)

           catcatATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAgcg                                                                                                                           <  2:1613248/140‑1 (MQ=255)

             tcatATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGACGACTGCATGTCACGGTGCCAGCGcc                                                                                                                         >  1:3343582/1‑140 (MQ=255)

               atatGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACAAAGAACTGTAAGAATGCATGACTCGGTGCCAGCACCAt                                                                                                                       >  1:3673593/1‑140 (MQ=255)

                tatGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCAtt                                                                                                                      <  1:7199132/140‑1 (MQ=255)

                  tGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCAtttt                                                                                                                    >  1:1308195/1‑140 (MQ=255)

                   gCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTa                                                                                                                   <  1:4539732/140‑1 (MQ=255)

                    cACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTAt                                                                                                                  >  1:1737054/1‑140 (MQ=255)

                     aCCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAg                                                                                                                             <  2:1879264/128‑1 (MQ=255)

                     aCCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATc                                                                                                                 >  2:4603561/1‑140 (MQ=255)

                       cAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCAtt                                                                                                                      <  2:4106263/133‑1 (MQ=255)

                       cAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCa                                                                                                               >  1:6127875/1‑140 (MQ=255)

                       cAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCa                                                                                                               >  1:230167/1‑140 (MQ=255)

                       cAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCa                                                                                                               >  2:4807455/1‑140 (MQ=255)

                       cAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCa                                                                                                               >  2:6246243/1‑140 (MQ=255)

                        aaTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAg                                                                                                              <  2:3956204/140‑1 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACTTAGAACTGTAAGAATGGATGACACGATGCCAGCGCCATTTTATCCAGt                                                                                                             >  2:3583502/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAGGAATGCAGGACTCGGTGCCAGGGCCATTTTATCCAGt                                                                                                             >  1:2182401/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGt                                                                                                             >  1:761643/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGt                                                                                                             >  2:4249841/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGt                                                                                                             >  2:5854385/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGt                                                                                                             >  2:6392824/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGt                                                                                                             >  2:7293448/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGt                                                                                                             >  2:6559703/1‑140 (MQ=255)

                         aTAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGGTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATCTTATCCAGt                                                                                                             >  2:4730080/1‑140 (MQ=255)

                          tAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGtt                                                                                                            <  1:726279/140‑1 (MQ=255)

                             cgcgTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGtttgc                                                                                                         <  2:1162641/140‑4 (MQ=255)

                               cgTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCt                                                                                                       <  1:4405616/140‑1 (MQ=255)

                               cgTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCt                                                                                                       >  2:7110268/1‑140 (MQ=255)

                               cgTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAAATGGAAGAAGGCAAGCCGTGGGGCCATCGCCCTTTTATCCAGTTCGCCt                                                                                                       >  2:3703271/1‑140 (MQ=255)

                                 tAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTAc                                                                                                     >  1:7464/1‑140 (MQ=255)

                                     tGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTc                                                                                                 <  1:5927010/140‑1 (MQ=255)

                                       gCAATGTTGAACGGCCGGCCGATGACGACTTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCa                                                                                               <  1:2424560/140‑1 (MQ=255)

                                        cAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCAc                                                                                              <  1:3769625/140‑1 (MQ=255)

                                        cAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCAc                                                                                              <  2:5616444/140‑1 (MQ=255)

                                          aTGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGc                                                                                            <  1:1206809/140‑1 (MQ=255)

                                              tGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGaa                                                                                        >  1:6923227/1‑140 (MQ=255)

                                              tGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGaa                                                                                        >  1:5173817/1‑140 (MQ=255)

                                               gAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGa                                                                                         <  2:6709143/138‑1 (MQ=255)

                                                 aCGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACa                                                                                     <  1:1652175/140‑1 (MQ=255)

                                                  cGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACaa                                                                                    >  2:1373080/1‑140 (MQ=255)

                                                     cAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTAACAAGTTCGCCTACGTAGCACGCTGAAACaataa                                                                                 >  2:3413148/1‑140 (MQ=255)

                                                       ggCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCGAGCGCCATATTAACCAATTCGCCTAGGTTCGACGCTGAAACAATAATg                                                                               >  2:1976804/1‑140 (MQ=255)

                                                       ggCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATg                                                                               >  2:7563096/1‑140 (MQ=255)

                                                       ggCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATGTTAGCCAGTTCGCCTAGGTTCCACGCTGAAACAATAATg                                                                               >  2:2753488/1‑140 (MQ=255)

                                                         ccGAGGAAGACGTCACTAGAGCTCTGATAAATCGGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATgcg                                                                             <  2:3269974/140‑1 (MQ=255)

                                                           gAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATgcggc                                                                           <  1:4142092/140‑1 (MQ=255)

                                                            aGGAAGACGTCACAGGAACGCTTATAAAGCTTGCACGAGCGTTTGCCGCCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATgcggcg                                                                          <  2:2145221/140‑1 (MQ=255)

                                                               aaGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGa                                                                       >  1:1906751/1‑140 (MQ=255)

                                                                  aCGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATc                                                                    >  2:7463767/1‑140 (MQ=255)

                                                                   cGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCACCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATcc                                                                   >  1:4854426/1‑140 (MQ=255)

                                                                       acaGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGt                                                               <  1:6559703/140‑1 (MQ=255)

                                                                            aGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGttt                                                          >  2:127829/1‑140 (MQ=255)

                                                                             gcgcTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGtttt                                                         <  1:7049698/140‑1 (MQ=255)

                                                                              cgcTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGttttt                                                        >  1:6678075/1‑140 (MQ=255)

                                                                                cTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCa                                                      >  1:5740756/1‑140 (MQ=255)

                                                                                cTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCa                                                      >  1:6466686/1‑140 (MQ=255)

                                                                                  gATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    <  2:5012789/140‑1 (MQ=255)

                                                                                  gATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    <  1:1480578/140‑1 (MQ=255)

                                                                                  gATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    <  2:4420134/140‑1 (MQ=255)

                                                                                  gATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    <  1:5241193/140‑1 (MQ=255)

                                                                                  gATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    <  1:1859569/140‑1 (MQ=255)

                                                                                  gATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    <  1:663019/140‑1 (MQ=255)

                                                                                     aaaGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAAAAATAATGCGGCGCGGATCCGGGTCGTTTTTCAGCTgg                                                 >  1:2773970/1‑140 (MQ=255)

                                                                                       aGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGtt                                               <  2:1699646/140‑1 (MQ=255)

                                                                                         cTGGCAAGAGAGTTTTCCGTCTACCTCATTGAACTGGCAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCa                                             <  1:2021803/140‑1 (MQ=255)

                                                                                          tGGCAAGAGAGTTTGCCTTCTGCCACATAGAGCTGGAAGTATGCATGATACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAg                                            <  2:2993083/140‑1 (MQ=255)

                                                                                          tGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAg                                            <  2:5380435/140‑1 (MQ=255)

                                                                                          tGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAg                                            <  2:5495034/140‑1 (MQ=255)

                                                                                            gCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGGATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTa                                          <  2:723394/140‑1 (MQ=255)

                                                                                            gCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGc                                                    >  1:5832211/1‑130 (MQ=255)

                                                                                            gCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTAAAACAATATTGCGGCGAGAATCCGGATCGTGTTTCAGCTGGTTCAGTa                                          >  2:2231649/1‑140 (MQ=255)

                                                                                            gCAAGAGAGTTTGCCATCTGCCGCATAGAGCTGGAAGTATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTa                                          <  1:3551790/140‑1 (MQ=255)

                                                                                             cAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTa                                          >  2:1833599/1‑139 (MQ=255)

                                                                                             cAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAAAGCGGCGCGAATCCGGGTCGTTCTTCAGCTGGTTCAGTAc                                         >  2:2536638/1‑140 (MQ=255)

                                                                                                 agagTTTGCCGTCTGCCTCATATAACTGGAATAATTCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTa                                     <  2:3108455/140‑1 (MQ=255)

                                                                                                 agagTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTa                                     <  2:146878/140‑1 (MQ=255)

                                                                                                     tttGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGa                                 <  1:4709792/140‑1 (MQ=255)

                                                                                                      ttGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGAt                                <  2:6923227/140‑1 (MQ=255)

                                                                                                      ttGCCGTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGAt                                >  1:1021580/1‑140 (MQ=255)

                                                                                                           gTCTGCTACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTa                                          >  2:912497/1‑125 (MQ=255)

                                                                                                           gTCTGCTACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTa                                          <  1:912497/125‑1 (MQ=255)

                                                                                                           gTCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGt                           >  1:259719/1‑140 (MQ=255)

                                                                                                            tCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTc                          <  2:259719/140‑1 (MQ=255)

                                                                                                            tCTGCCACATAGAACTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTc                          <  1:1089534/140‑1 (MQ=255)

                                                                                                                        aaCTGGAAGAATGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAatat                     >  2:5197522/1‑133 (MQ=255)

                                                                                                                             gaagaaTGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGAAGTGATCTGGTCAATATGACGACCATCTg         >  1:2870875/1‑140 (MQ=255)

                                                                                                                              aagaaTGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTgg        >  1:4244953/1‑140 (MQ=255)

                                                                                                                                gaaTGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCg      <  2:6010363/140‑1 (MQ=255)

                                                                                                                                  aTGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTAGCGTAGTGATCTGGTCAATATGACGACCATCTGGCGtt    >  1:3029499/1‑140 (MQ=255)

                                                                                                                                  aTGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACTATCTGGCGtt    >  2:5712568/1‑140 (MQ=255)

                                                                                                                                  aTGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCg      >  2:5141553/1‑138 (MQ=255)

                                                                                                                                   tGCATGACACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTg   >  2:4208159/1‑140 (MQ=255)

CACTGCTGCGCCATCATATGCACCAATAACGCGTAGCTGGCAATGTTGAACGGCAGGCCGAGGAAGACGTCACAGGAGCGCTGATAAAGCTGGCAAGAGAGTTTGCCGTCTGCCACATAGAACTGGAAGAATGCATGGCACGGTGCCAGCGCCATTTTATCCAGTTCGCCTACGTTCCACGCTGAAACAATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGG  >  NC_000913/2964581‑2964852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`