Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,231,417	A→G	intergenic (+178/‑248)	ygjK → / → fadH	glycoside hydrolase/2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,231,417	0	A	G	100.0%	170.0 / NA	55	intergenic (+178/‑248)	ygjK/fadH	glycoside hydrolase/2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

CCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCGTATTC  >  NC_000913/3231381‑3231553                                     |                                                                                                                                          ccTACATGTGCAATCCTGCTGGTCGGATAAATCGCTGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa                                    <  2:2114466/140‑1 (MQ=14)                  cctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGGCGCCTTATTCCCCCTACATGGGCAATTCCGTAACCCGGATAAAAACCGACAAACG‑GCGCAACCCGCGtt                  >  2:5058003/7‑140 (MQ=14)                  cctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGtt                  <  1:918269/134‑1 (MQ=14)                  cctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGtt                  >  1:5214865/7‑140 (MQ=14)                   ctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTa                 >  1:121505/6‑140 (MQ=14)                   ctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTa                 >  2:3737079/6‑140 (MQ=14)                   ctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCCCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTa                 >  2:4072592/6‑140 (MQ=14)                   ctggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGGGGCCGCCTTATCCGCCCTACATGTGCAATTTCGGAAACCGCGTAAAACGCGACAAGCGATCGC‑TCCGGCCTTa                 >  2:1718107/6‑140 (MQ=11)                     ggccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGGCGCCTTATCCCCCCTACAAGTGCAATTACGTAACCCGGATAAAAAACGACAAACG‑GCGCATACCGCGTTATc               >  2:4195700/4‑140 (MQ=14)                      gccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTTGATAAAACGCGACAAGCG‑TCGCATCCGGCGGTATcc              >  1:1770854/3‑139 (MQ=17)                      gccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCa              >  1:4932547/3‑140 (MQ=255)                       ccGGATAAAACGCGGTAAGCGTAGCTTCCTGCATCTGGACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  1:2277432/139‑1 (MQ=18)                       ccGGATAAAACGCGGCATGCGTTGCATCCGGTATCAGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:2384884/139‑1 (MQ=17)                       ccGGATAAAACGCGGCATGCGTTGCATCCGGCATCTGCATCGCGCCACGGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:2165645/139‑1 (MQ=17)                       ccGGATAAAACGCGGCAAGGTTTGCGGCCGGTAACGGCGTGGCACCACGGGCGGATGCGGCGTTGACGCCTTATCCGCCCTACATGTGCAATTCCGTAAGCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:3158236/139‑1 (MQ=14)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  1:4274894/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  1:1679224/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:7209046/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:1301679/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:6646479/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  1:5714161/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:6143075/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:464467/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:5662339/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:5185907/139‑1 (MQ=255)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCACCACTGGCGGATGCGGTGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  1:6670229/139‑1 (MQ=17)                       ccGGATAAAACGCGGCAAGCGTTGCATCCGGCATCCGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:191710/139‑1 (MQ=255)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACCTGTGCAATTCCGGAACCCGGATAAAACGCGACAAACG‑TCGCATCCGGCGTTATCAcc            >  2:6552388/1‑140 (MQ=17)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAcc            <  2:818118/140‑1 (MQ=255)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAcc            >  2:6399018/1‑140 (MQ=255)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAcc            >  2:6302241/1‑140 (MQ=255)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAcc            >  1:5096570/1‑140 (MQ=255)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAcc            >  1:5124737/1‑140 (MQ=255)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATGCCGTAACCGGGAGAAAACGCGACAAGCG‑TCTCATCCGGCGTTATCAcc            >  2:2062030/1‑140 (MQ=17)                        cGGATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGGGCAATTCCGTAACCTGGGTAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAcc            >  2:1474315/1‑140 (MQ=17)                          gATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCCTCCGGCGTTATCAc             <  2:4646379/137‑1 (MQ=255)                          gATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             >  1:4646379/1‑137 (MQ=255)                          gATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCgg          >  1:6961357/1‑140 (MQ=255)                          gATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCgg          >  1:7379482/1‑140 (MQ=255)                          gATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCgg          >  1:3953964/1‑140 (MQ=255)                          gATAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCCCCCTACATGTGCAATTCACTAACCCGGATAAAACCCGACAAACG‑TCCCATCCGGCGTTATCAcccg          >  2:2326688/1‑138 (MQ=17)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGGGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  1:6799140/136‑1 (MQ=255)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATTAAACGCGACAATCG‑ATGCATCAGGCGTTTTCACCggg         >  1:3205331/1‑140 (MQ=17)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             <  2:1763451/136‑1 (MQ=255)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCAc             >  1:1763451/1‑136 (MQ=255)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCggg         >  1:7378596/1‑140 (MQ=255)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCggg         >  2:3743723/1‑140 (MQ=255)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAACG‑TCGCATCCGGCGTTATCAc             >  2:6799140/1‑136 (MQ=255)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCCTAACCCGGATAAAACGCGACAAACG‑TCGCATCCGGCGTTATCACCggg         >  2:5339873/1‑140 (MQ=17)                           aTAAAACGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCATAACCTGGGTAACAAGCGACAAACG‑GCGCATCCGGCGGTATCACCggg         >  2:2498945/1‑140 (MQ=17)                             aaaaCGCGGCAAGCGTTTCATCTGGCATCTGAACCTCGCTACTGGCGGCTGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCg       <  2:2042530/137‑1 (MQ=18)                             aaaaCGCGGCAAGCGTTGCATCCGGTATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCg       <  1:3716085/137‑1 (MQ=255)                             aaaaCGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCg       <  2:1422552/137‑1 (MQ=255)                                aCGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCGCTGGCGGATGCGGCGTGGACGCCTTATCCGTCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCGTAt    <  2:3882649/140‑1 (MQ=255)                                aCGCGGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCGTAt    <  1:5499261/140‑1 (MQ=255)                                  gcgGCAAGCGTTGCATCGGGCAGCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCGTATTc  <  2:3133345/140‑1 (MQ=255)                                  gcgGCAAGCGTTGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCGTATTc  <  1:5918753/140‑1 (MQ=255)                                     |                                                                                                                                          CCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCG‑TCGCATCCGGCGTTATCACCGGGCGTATTC  >  NC_000913/3231381‑3231553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑