BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,250,696	T→C	G45G (GGT→GGC)	yqjF →	DoxX family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,250,696	0	T	C	99.6%	1640.6 / NA	444	G45G (GGT→GGC)	yqjF	DoxX family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (257/184); minor base A (0/2); total (257/187)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 454 total aligned reads displayed.

TGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAGCAACTTTG  >  NC_000913/3250560‑3250832

tgatgaAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGcttt                                                                                                                                       <  1:1606840/140‑1 (MQ=255)

 gatgaAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTa                                                                                                                                      >  1:5258736/1‑140 (MQ=255)

 gatgaAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTa                                                                                                                                      >  1:126575/1‑140 (MQ=255)

  atgaAAAAATTAGAACATGTTGATGCACTCGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTAt                                                                                                                                     <  1:3503004/140‑1 (MQ=255)

   tgaAAAAATTAGAAGATTTTGGCGTACTGGTAGCGCGCACTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTAtg                                                                                                                                    <  2:2383145/140‑1 (MQ=255)

   tgaAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACGCAACAATCTATGGAAGCAATGGGCGTCCCGGGCTTTAtg                                                                                                                                    >  2:2617533/1‑140 (MQ=255)

   tgaAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTAtg                                                                                                                                    >  2:5332926/1‑140 (MQ=255)

   tgaAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTAtg                                                                                                                                    >  2:6606654/1‑140 (MQ=255)

      aaaaaTTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGTAAGCAAAGGGCGTACCGGGCTTTAtgctg                                                                                                                                 >  1:3031331/1‑140 (MQ=255)

      aaaaaTTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTAtgc                                                                                                                                   >  2:5398370/1‑138 (MQ=255)

      aaaaaTTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCAGATACTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTAtgctg                                                                                                                                 >  2:1844301/1‑140 (MQ=255)

        aaaTTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGcc                                                                                                                               <  2:5258736/140‑1 (MQ=255)

             agaagaTGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGACTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTgg                                                                                                                          <  2:7045300/140‑1 (MQ=255)

                    gTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCt                                                                                                                   >  1:561439/1‑140 (MQ=255)

                     ttGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTg                                                                                                                  <  2:1157003/140‑1 (MQ=255)

                      tGGTGTACTGGTAGCGCGCATTGTAATGCCGCTTCCGTTCATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGc                                                                                                                 <  2:2119500/140‑1 (MQ=255)

                           tACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAg                                                                                                            >  1:6100577/1‑140 (MQ=255)

                             cTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCtt                                                                                                               >  2:4456052/1‑135 (MQ=255)

                             cTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGtt                                                                                                          >  2:7344041/1‑140 (MQ=255)

                             cTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGtt                                                                                                          >  1:1018684/1‑140 (MQ=255)

                             cTGGTAGCGCGCATTTTAATGCCGATTCTGTCTATGACCTCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGtt                                                                                                          <  2:2760511/140‑1 (MQ=255)

                                gcaGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATAGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtgg                                                                                                       >  1:1517021/3‑140 (MQ=255)

                                gTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtgg                                                                                                       >  1:1088226/1‑140 (MQ=255)

                                gTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGG‑AAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggt                                                                                                      >  1:5053220/1‑140 (MQ=255)

                                  aGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtg                                                                                                     >  1:998986/1‑140 (MQ=255)

                                  aGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtg                                                                                                     <  2:4757431/140‑1 (MQ=255)

                                  aGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGG‑AAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtgg                                                                                                    <  2:5053220/140‑1 (MQ=255)

                                      cgcATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtggtgg                                                                                                 >  1:5408324/1‑140 (MQ=255)

                                      cgcATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtggtgg                                                                                                 <  1:89071/140‑1 (MQ=255)

                                      cgcATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtggtgg                                                                                                 >  1:5965159/1‑140 (MQ=255)

                                      cgcATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTtggtggtgg                                                                                                 <  2:906439/140‑1 (MQ=255)

                                          ttttAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTg                                                                                             <  1:4442778/140‑1 (MQ=255)

                                          ttttAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCGGCTTGAGTTTGGTGGTGGTCTg                                                                                             >  2:2471768/1‑140 (MQ=255)

                                             tAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCa                                                                                          <  2:681260/140‑1 (MQ=255)

                                              aaTGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCaa                                                                                         <  2:1758929/140‑1 (MQ=255)

                                                     aTTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGtt                                                                                  >  2:6078760/1‑140 (MQ=255)

                                                            ttattaCCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTc                                                                           <  1:6625175/140‑1 (MQ=255)

                                                                taCCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGCTTCTGCTTTAGTCTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGa                                                                       >  2:2876253/1‑140 (MQ=255)

                                                                taCCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGa                                                                       >  2:4118584/1‑140 (MQ=255)

                                                                  ccGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACt                                                                     <  1:4266910/140‑1 (MQ=255)

                                                                   cGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTc                                                                    >  1:4875354/1‑140 (MQ=255)

                                                                     ctggctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTTCTTGAGTTGGGTGGTGTTCTGGCAAACCTGTTCGGTTTCCTGACTCGc                                                                  >  2:3232843/1‑140 (MQ=255)

                                                                     ctggctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTg                                                                        <  2:776210/134‑1 (MQ=255)

                                                                     ctggctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCg                                                                   <  2:272129/139‑1 (MQ=255)

                                                                     ctggctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGc                                                                  >  2:1346948/1‑140 (MQ=255)

                                                                     ctggctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGc                                                                  >  1:6386507/1‑140 (MQ=255)

                                                                     ctggctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGATGGTGGTGTGGGAAGCCTGTTCGGTTTCCTGACTCGc                                                                  >  1:2217999/1‑140 (MQ=255)

                                                                       ggctggGGAAAAATTGCTGGCTCCGAGGGGACCCAACCATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGcac                                                                <  1:2617533/140‑1 (MQ=255)

                                                                         ctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATTCTGTTCGGTTTCCTGACTCGcacca                                                              >  1:3620521/1‑140 (MQ=255)

                                                                         ctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGcacca                                                              <  1:7367062/140‑1 (MQ=255)

                                                                         ctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGcacca                                                              <  1:5906288/140‑1 (MQ=255)

                                                                         ctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGcacca                                                              <  2:5965159/140‑1 (MQ=255)

                                                                         ctggGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGcacca                                                              <  1:3905518/140‑1 (MQ=255)

                                                                         ctggGGAAAAATTACTGGCTACGCGGGTACCCAACAAGATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGcacca                                                              <  1:3232843/140‑1 (MQ=255)

                                                                           ggggAAAAATTACTGGCTACGCTGGTACCCAACAATAGATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACcaca                                                            <  1:2395838/140‑1 (MQ=255)

                                                                           ggggAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACcaca                                                            <  1:1760236/140‑1 (MQ=255)

                                                                           ggggAAAAATTACTGCCTACGCCGGCACCCAACAATACATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACcaca                                                            <  1:2011715/140‑1 (MQ=255)

                                                                             ggAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGTAATCCTGTTCGGTTTCCTGACTCGCACCACAGc                                                          <  2:4266502/140‑1 (MQ=255)

                                                                             ggAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCg                                                                   <  1:3765883/131‑1 (MQ=255)

                                                                             ggAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGc                                                          <  2:157080/140‑1 (MQ=255)

                                                                             ggAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGc                                                          <  1:28319/140‑1 (MQ=255)

                                                                             ggAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGc                                                          <  1:1851063/140‑1 (MQ=255)

                                                                              gAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGcc                                                         <  2:1088226/140‑1 (MQ=255)

                                                                                        tGGCTACGCGGGTACCTAACAATATATGGACCCAACGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTg                                               <  2:3690583/140‑1 (MQ=255)

                                                                                        tGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTg                                               <  1:5737095/140‑1 (MQ=255)

                                                                                         ggCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGc                                              <  1:7534505/140‑1 (MQ=255)

                                                                                            tACGCGGGTACCCAACATTATATGGAAGCAATGGGGGTCTCGGGCTTTTTGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCggg                                           <  2:3006285/140‑1 (MQ=255)

                                                                                              cgcgGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCATTCCTGTTCCGTTTCCTGACTCGCACCACAGCACTGTTTACTGCGGGCt                                         >  2:3318156/1‑140 (MQ=255)

                                                                                              cgcgGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCt                                         >  2:5648926/1‑140 (MQ=255)

                                                                                                           caaTATATGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGAc                            >  1:7037726/1‑140 (MQ=255)

                                                                                                             atatatGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACgg                          >  2:5853693/1‑140 (MQ=255)

                                                                                                              tatatGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGc                         <  1:5144206/140‑1 (MQ=255)

                                                                                                              tatatGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGc                         <  1:4118584/140‑1 (MQ=255)

                                                                                                                tatGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCAt                       >  1:1688346/1‑140 (MQ=255)

                                                                                                                tatGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCAt                       <  2:815612/140‑1 (MQ=255)

                                                                                                                 atGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCAtt                      >  2:6697282/1‑140 (MQ=255)

                                                                                                                  tGGAAGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCAttt                     >  1:431171/1‑140 (MQ=255)

                                                                                                                   gggaGCAATGGGCTTCCCGGGCTTTATGCTGCCACTGGTAATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTAACTCGCACCAGAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCAtttt                    >  2:4346428/4‑140 (MQ=255)

                                                                                                                     aaGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTa                  >  1:4613018/1‑140 (MQ=255)

                                                                                                                     aaGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTa                  >  2:3914546/1‑140 (MQ=255)

                                                                                                                     aaGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGGTAAGAGCGGGCTGTACGTCGCTGACGACATTTTTa                  >  2:3606517/1‑140 (MQ=255)

                                                                                                                      aGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTAt                 >  2:7426426/1‑140 (MQ=255)

                                                                                                                      aGCAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTAt                 >  1:1416446/1‑140 (MQ=255)

                                                                                                                        cAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTAttt               >  2:1454178/1‑140 (MQ=255)

                                                                                                                        cAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTAttt               >  2:3783705/1‑140 (MQ=255)

                                                                                                                        cAATGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTAttt               <  2:6386507/140‑1 (MQ=255)

                                                                                                                          aTGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTca             >  2:1621077/1‑140 (MQ=255)

                                                                                                                          aTGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTca             >  2:6474543/1‑140 (MQ=255)

                                                                                                                          aTGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTca             >  2:5595590/1‑140 (MQ=255)

                                                                                                                          aTGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTATACAGAGGGCTTTACGTTGCTATCGGCATTTTTATTTca             >  2:3468092/1‑140 (MQ=255)

                                                                                                                           tGGGCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTcac            >  1:6766738/1‑140 (MQ=255)

                                                                                                                             ggCGTCCCGGGCTTTGTGCTGCAACTGGTCATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAg          <  1:3441265/140‑1 (MQ=255)

                                                                                                                             ggCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAg          <  1:4783927/140‑1 (MQ=255)

                                                                                                                             ggCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAg          <  1:5340639/140‑1 (MQ=255)

                                                                                                                             ggCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAg          <  2:4613018/140‑1 (MQ=255)

                                                                                                                              gCGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAGc         <  2:4934215/140‑1 (MQ=255)

                                                                                                                               cGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTTGTGGTCTGGCAATGCTGTTCGGTTTCCTGACTCGACTCACAGCCCTGTTTACTGCCGGCTTTTCGCTGCTGACGGAATTTTTATTTCACCGcc        <  2:4284930/140‑2 (MQ=255)

                                                                                                                               cGTCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAGCa        >  1:774948/1‑140 (MQ=255)

                                                                                                                                 tCCCGGGCTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGG‑GGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAGCAACt     >  1:6517775/1‑140 (MQ=255)

                                                                                                                                 tCCCGGGCTTTATGCGGCCACTGGTTATTTTGCTTGAATTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGATTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAGCAAc      <  1:2739595/140‑1 (MQ=255)

TGATGAAAAAATTAGAAGATGTTGGTGTACTGGTAGCGCGCATTTTAATGCCGATTCTGTTTATTACCGCTGGCTGGGGAAAAATTACTGGCTACGCGGGTACCCAACAATATATGGAAGCAATGGGCGTCCCGGGTTTTATGCTGCCACTGGTGATTCTGCTTGAGTTTGGTGGTGGTCTGGCAATCCTGTTCGGTTTCCTGACTCGCACCACAGCCCTGTTTACTGCGGGCTTTACGCTGCTGACGGCATTTTTATTTCACAGCAACTTTG  >  NC_000913/3250560‑3250832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`