BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,294,876	T→C	S493P (TCT→CCT)	lpoA →	outer membrane lipoprotein ‑ activator of MrcA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,294,876	0	T	C	100.0%	718.2 / NA	198	S493P (TCT→CCT)	lpoA	outer membrane lipoprotein ‑ activator of MrcA activity
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (3/195); total (3/195)

Only 100 of 280 total aligned reads displayed.

GATCCCACGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGT  >  NC_000913/3294743‑3295012

gATCCCACGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTa                                                                                                                                      <  1:373390/140‑1 (MQ=255)

gATCCCACGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGGTcctgga                                                                                                                                      >  1:6890362/1‑135 (MQ=255)

   cccACGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTg                                                                                                                                   <  1:1194662/140‑1 (MQ=255)

      aCGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑tt                                                                                                                               >  1:1116297/1‑140 (MQ=255)

      aCGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGGTTGC‑tt                                                                                                                               >  1:3877020/1‑140 (MQ=255)

       agacagtCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑ttt                                                                                                                              <  1:5228890/134‑1 (MQ=255)

        gCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTa                                                                                                                             <  1:6686818/140‑1 (MQ=255)

           gTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTATGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTCTTAACGACGGTCCTGGGATTTT‑TTTAACg                                                                                                                          >  1:3640329/1‑140 (MQ=255)

            ttCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTGCCTGTATTTC‑TTTAAAgg                                                                                                                         >  1:7570347/1‑140 (MQ=255)

            ttCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTTGGATTTC‑tttttccg                                                                                                                         >  2:3816903/1‑135 (MQ=255)

            ttCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGATTGC‑ttttaagg                                                                                                                         >  1:6032984/1‑135 (MQ=255)

             tCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCTCGGGTGTCAACTGCCGTCCTGTGATTTC‑TTTTAAggg                                                                                                                        >  1:2527636/1‑140 (MQ=255)

              cATTGGGCGATCGCGTAGCCAATGCGTTTGCCCAAGAGTTGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGt                                                                                                                       <  2:2478125/140‑1 (MQ=255)

              cATTGGGCGATCGCGCAGCCAATGCTTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGt                                                                                                                       <  1:2847870/140‑1 (MQ=255)

               aTTGGGCGATCGCGTTGCCAATACGGTTGCGCAAGAGCGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTa                                                                                                                      <  1:3320478/140‑1 (MQ=255)

                ttGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAg                                                                                                                     <  1:167952/140‑1 (MQ=255)

                ttGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAg                                                                                                                     <  1:7154456/140‑1 (MQ=255)

                ttGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAg                                                                                                                     <  2:290229/140‑1 (MQ=255)

                 tGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGc                                                                                                                    <  1:5567672/140‑1 (MQ=255)

                  gggCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGcc                                                                                                                   <  2:127360/140‑1 (MQ=255)

                    gCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCg                                                                                                                 <  2:314724/140‑1 (MQ=255)

                      gATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTCACGGCGGACCTTGGTTTGC‑TTGTAAGGGTGGCCCGAt                                                                                                               >  1:2314632/1‑140 (MQ=255)

                      gATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGAt                                                                                                               <  1:6315839/140‑1 (MQ=255)

                      gATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGAt                                                                                                               <  2:4600048/140‑1 (MQ=255)

                      gATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGATTTT‑TTTTAAGGGGAGGCCgcg                                                                                                               >  2:7087689/1‑138 (MQ=255)

                      gATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGGCCTGGGAGTTC‑TTTTACGGGGGGccccgt                                                                                                               >  2:5751513/1‑137 (MQ=255)

                      gATCGCGTAGCCAATCCGTTTGTGCCAGACTGGCAGACACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGAt                                                                                                               <  1:2061562/140‑1 (MQ=255)

                          gcgTAGCCAATGCGTTTCCGCACGAGTGGCACAAATTGGACGGCGGCAGCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTAct                                                                                                           <  2:2160631/140‑1 (MQ=255)

                          gcgTAGCCAATGCGTTTCCGCAAGAGTGGCAAAAATTGGCCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTAct                                                                                                           <  2:2002038/140‑1 (MQ=255)

                               gCCAATGCTTTTGCGCTAGAGTTGCAGAAACTGGGTGGCCGCACCGTTTTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCag                                                                                                      <  2:3555335/140‑1 (MQ=255)

                               gCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCag                                                                                                      <  2:1582794/140‑1 (MQ=255)

                                 cAATGCGTTTGCGCATGAGTGGGAGTAACGGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCagag                                                                                                    <  1:3132548/140‑1 (MQ=255)

                                    tGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑Ga                                                                                                >  1:4758850/1‑140 (MQ=255)

                                    tGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTAGTAGTGC‑ATTTAAGGGAGGCCCCCTTACTCTCACAGC‑Ga                                                                                                >  2:2666431/1‑140 (MQ=255)

                                    tGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGGCCTGGGATTTC‑TTTTACGGGTAGCCCCATTACTCCTagagagg                                                                                                 >  1:6950951/1‑137 (MQ=255)

                                        tttGCGCAAGAGTGGGAGAAACGGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAAc                                                                                            <  1:2491684/140‑1 (MQ=255)

                                        tttGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTGTTTC‑TTTTACGGGGAGCCCGCGTAATCTCCGAGC‑GGCAAc                                                                                            >  2:6992013/1‑140 (MQ=255)

                                          tGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTTGGATTTCTTTTAAGGGGGGGCCCG‑TTACTCTCAGAGG‑GGCaaacc                                                                                          >  2:5338382/1‑137 (MQ=255)

                                          tGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTTACGGGGAGGCCCATTACTCTCAGAGC‑GACAAccc                                                                                          >  1:805249/1‑139 (MQ=255)

                                          tGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTTACGGGGAGCCCGATTACTCTCCGAGC‑GGCAAccc                                                                                          >  1:4206183/1‑139 (MQ=255)

                                                agagTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTTACGGGGAGCCCGATTTCTCTCAGAGC‑GGCAACCGACTccc                                                                                    >  1:440808/1‑139 (MQ=255)

                                                   gTGGCAGACACTGGGCTACGGCTCCGTTATTGACCGAAATTTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCa                                                                                 <  2:3640329/140‑1 (MQ=255)

                                                   gTGGCAGAAACTGGGTGGCGGCCCCATTCAGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCa                                                                                 <  1:3198996/140‑1 (MQ=255)

                                                   gTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCa                                                                                 <  1:5080122/140‑1 (MQ=255)

                                                   gTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCa                                                                                 <  2:3917542/140‑1 (MQ=255)

                                                      gCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTCGTAATACTTTTAACGGG‑AGGCCGCTTAATCTTCAAGA‑GACGACCGAcccccggctta                                                                              >  2:663278/1‑133 (MQ=255)

                                                        aGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATgacg                                                                            <  2:4409965/140‑1 (MQ=255)

                                                        aGAAACTGGGCGGCGGCACCGTACTTCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATgacg                                                                            <  1:2935340/140‑1 (MQ=255)

                                                           aaCTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGAcc                                                                         <  1:6269394/140‑1 (MQ=255)

                                                           aaCTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGAcc                                                                         <  2:6784280/140‑1 (MQ=255)

                                                             cTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACcaa                                                                       <  1:3796695/140‑1 (MQ=255)

                                                              tGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGTTTTC‑TTTTACGGGTAGCCCGGTTACTCTCCGAGG‑GGCAACCCACTCCCGCATGACGACcaac                                                                      >  2:4037337/1‑140 (MQ=255)

                                                                ggcggcggcACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGATTTC‑TTTTACCGGGAGGCCGATTACTCTCAGAGC‑GACAACCCAACCCCGCACGACCACcaaaaa                                                                    >  2:6189137/1‑140 (MQ=255)

                                                                     cggcACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTTGTGTTGC‑TTTTACGGGTGGGCCGTCTACTCTCTGAGG‑GCCAACCGACCCTCGCGGGTCGaccaaccaaccac                                                               >  2:2284760/1‑139 (MQ=255)

                                                                     cggcACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCaa                                                               <  2:4837860/140‑1 (MQ=255)

                                                                     cggcACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCaa                                                               <  2:1686540/140‑1 (MQ=255)

                                                                     cggcACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGGTTTC‑TTTTACAGGGAGCCCCGTTACTCTCAGAGC‑GGCAACCCACTCCCGCCTGACGACCAACAAACCaa                                                               >  2:5229818/1‑140 (MQ=255)

                                                                         aCCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCt                                                           <  1:6189137/140‑1 (MQ=255)

                                                                             ttCTGCAACAAAAATTTGGTTCGACAAGCGAATTACGTGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCaa                                                       <  1:2666431/140‑1 (MQ=255)

                                                                               cTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTTACGGGGAGCCCCCTTTCTCTCCGAGC‑GACAAACCACTCCCGCATGACGACCAACAAACCAACCCCGCAAAc                                                     >  2:6399942/1‑140 (MQ=255)

                                                                               cTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGATTGC‑TTTTACGGGGAGCCCCGTTACTCTCAGAGC‑GGCAACCGACTCCCGCATGACGACCAACAAACCAACACTGCCAAc                                                     >  2:1211374/1‑140 (MQ=255)

                                                                                 gCAACAAAAATTTGGTTCCACCTGCGAATTACGCGGGGGCGTTAACGGAGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCa                                                   <  2:2527636/140‑1 (MQ=255)

                                                                                  caacaaAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGATTTC‑CTTTACGGGGAGCCCCCTTTCTCTCCGAGC‑GACAAACCACTCCCGCAGGCCCACCCAAAAACCCACGCTCCAAAacac                                                  >  2:695936/1‑140 (MQ=255)

                                                                                     caaAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTTACGGGGAGCCCCGTTACTCTCCCAGC‑GGCAACCCACTCCCGCCTGACGACCAACAAACCAACCCTGCAAACCAcccc                                               >  2:5864175/1‑140 (MQ=255)

                                                                                     caaAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGcc                                               <  2:83172/140‑1 (MQ=255)

                                                                                     caaAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGcc                                               <  2:5441589/140‑1 (MQ=255)

                                                                                        aaaTTTGGTTCCACCCGCGAATATCACGCCGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAAc                                            <  1:2925709/140‑1 (MQ=255)

                                                                                               gTTCCACCAGCGAATTACGCGCGGGTGTTACCGGCTGTCGTGGTATTGT‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                     <  2:3483511/140‑1 (MQ=255)

                                                                                               gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                     <  1:1906591/140‑1 (MQ=255)

                                                                                               gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAc                                     <  2:5577158/140‑1 (MQ=255)

                                                                                               gTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGACCTGGGGTTTC‑TTTTAAGGGTAACCCCATTACTCCCCGAGCGGGCAACGCAC‑CGCGCACGACCACCTAAAAACCAACGCCGGAAACCAAGCCCACCCAAGAc                                     >  2:2700154/1‑140 (MQ=255)

                                                                                                ttCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGAcc                                    <  2:4699645/140‑1 (MQ=255)

                                                                                                 tCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGGGTTTC‑TTTTACGGGGAGCCCGCTTACTCTCAGAGC‑GGCAAACCACTCCCGCACGACGACCAACAAACCAACACCGCAAAACACGCCAACCCATGAccc                                   >  2:4162832/1‑139 (MQ=255)

                                                                                                  ccaccaGCGAATTACACGCGGGTGTCAACAGCGGTCCAGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGGGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAg                                  <  1:2423273/140‑1 (MQ=255)

                                                                                                   caccaGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGt                                 <  1:4458906/140‑1 (MQ=255)

                                                                                                    cccaGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGtt                                <  1:1232590/139‑1 (MQ=255)

                                                                                                     ccaGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTc                               <  2:4210531/140‑1 (MQ=255)

                                                                                                     ccaGCGAATTACGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTc                               <  1:1882550/140‑1 (MQ=255)

                                                                                                               aCGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATg                     <  1:6452608/140‑1 (MQ=255)

                                                                                                               aCGCGCGGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATg                     <  1:3965690/140‑1 (MQ=255)

                                                                                                                   gcgGGTGTTAACGGCGGGCCTGATATTGC‑TTAAACAGGTCGCCCGATTGCTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCgg                 <  2:2594059/140‑1 (MQ=255)

                                                                                                                    cgGGTGTTAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGt                <  1:1612293/140‑1 (MQ=255)

                                                                                                                           tAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCg         <  2:7261442/140‑1 (MQ=255)

                                                                                                                           tAACGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCg         <  1:484033/140‑1 (MQ=255)

                                                                                                                           tAACGGCGGTCCTGGTATTGC‑TGTAACGGGTTGCCCGATTACTCTCAGTGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCg         <  2:2836706/140‑1 (MQ=255)

                                                                                                                            aaCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGa        <  1:6992013/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:5319273/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  2:7570347/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:5824265/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:88938/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:5456261/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:6619452/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTAGTGC‑TTTCACGGGTGGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:2722796/140‑1 (MQ=255)

                                                                                                                             aCGGCGGTCCTGGTACTGC‑TTTCACTGGTCGCCCGATGACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGAt       <  1:3515472/140‑1 (MQ=255)

                                                                                                                              cggcggTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATg      <  1:4037337/140‑1 (MQ=255)

                                                                                                                              cggcggTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATg      <  2:1116665/140‑1 (MQ=255)

                                                                                                                              cggcggTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATg      <  2:329114/140‑1 (MQ=255)

                                                                                                                              cggcggTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATg      <  1:5681569/140‑1 (MQ=255)

                                                                                                                               ggcggTCCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGc     <  2:4965455/140‑1 (MQ=255)

                                                                                                                                 cggTCCTGGGATTGC‑TTTAACGGGGAGCCCCGTTACTCTCAGAGC‑GGCAACCGACTCCCGCATGACGACCAACAATCCAACGCTGCAAAACCCGCCCACCTAAGTCCACATCACCAAGAAAGACGGGCCGGTCGAGGCgg   >  2:2387352/1‑140 (MQ=255)

GATCCCACGCAGTTCATTGGGCGATCGCGTAGCCAATGCGTTTGCGCAAGAGTGGCAGAAACTGGGCGGCGGCACCGTTCTGCAACAAAAATTTGGTTCCACCAGCGAATTACGCGCGGGTGTTAACGGCGGTTCTGGTATTGC‑TTTAACGGGTAGCCCGATTACTCTCAGAGC‑GACAACCGACTCCGGCATGACGACCAACAATCCAACGCTGCAAACCACGCCAACCGATGACCAGTTCACCAATAATGGCGGTCGTGTCGATGCGGT  >  NC_000913/3294743‑3295012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`