BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,584,131	T→A	L152Q (CTG→CAG)	insB‑6 →	IS1 protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,584,131	0	T	A	97.7%	858.8 / NA	270	L152Q (CTG→CAG)	insB‑6	IS1 protein InsB
Reads supporting (aligned to +/- strand): ref base T (0/2); major base A (93/167); minor base C (0/6); total (93/177)
Fisher's exact test for biased strand distribution p-value = 9.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

CCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGAT  >  NC_000913/3584063‑3584267

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGt                                                              >  2:7198263/1‑140 (MQ=37)

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGt                                                              >  1:7139934/1‑140 (MQ=37)

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGt                                                              >  1:1239581/1‑140 (MQ=37)

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGt                                                              >  2:3907339/1‑140 (MQ=37)

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGAAGTCATTACCGACATTAAAAGt                                                              >  1:2860808/1‑140 (MQ=255)

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGCTGAAGTCATGACCGACATTAAAAGt                                                              >  1:3215978/1‑140 (MQ=255)

     aTCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAAGAAGGTGGAGTCATGACCGACATTAAAAGt                                                              >  1:2394527/1‑140 (MQ=255)

          aGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCACTAATTTGGAGTCATTACCGACATTAAAAGTAACtt                                                         >  2:2682380/1‑140 (MQ=255)

          aGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACtt                                                         >  1:5309680/1‑140 (MQ=255)

          aGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACAAAAAACACTTTCAATAAGTTGGAGTCATTACGTACATTAAGAGTAACtt                                                         >  2:3714770/1‑140 (MQ=11)

           ggCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATCAAACACTACCAACAAGTTGGAGTCAGTACCTACATTAAAAGTAACttt                                                        >  2:2860879/1‑140 (MQ=14)

             cagcaCCTGGCACGGCTGGGACTGAAGTCGCCGTCTTTCCCAAAATCGTTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACttttt                                                      <  2:2890574/140‑1 (MQ=14)

              agcaCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAATCACTATCAGTAAATTGAAGTCATTAACGACATTAAAAGTAACtttttt                                                     >  2:2016681/1‑140 (MQ=14)

               gcaCCTGGCACGGCTTGGACGGCAGCCGCCGTCGTTCCCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTg                                                    <  1:2036138/140‑1 (MQ=14)

               gcaCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTg                                                    >  2:103286/1‑140 (MQ=255)

               gcaCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTg                                                    <  2:6784961/140‑1 (MQ=255)

                caCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGa                                                   >  2:1083341/1‑140 (MQ=255)

                caCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGa                                                   >  1:671378/1‑140 (MQ=255)

                caCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGa                                                   >  1:787127/1‑140 (MQ=255)

                  ccTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGaaa                                                 <  2:3998876/140‑1 (MQ=255)

                  ccTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGaaa                                                 <  1:4550552/140‑1 (MQ=255)

                   cTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAt                                                <  1:5696487/140‑1 (MQ=255)

                   cTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAt                                                <  1:5538901/140‑1 (MQ=255)

                   cTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAt                                                <  2:1337945/140‑1 (MQ=255)

                   cTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAt                                                <  2:1891855/140‑1 (MQ=255)

                    tGGCACGGCTGGGACTGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  1:2608319/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:6032709/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:4276475/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:4750472/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:358708/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  1:4909001/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:565041/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCGCTGTCGTTATCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:6594320/140‑1 (MQ=255)

                    tGGCACGGCTGGGACGGAAGTCCCTTTCGCTCTCAAACTCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  1:3588621/140‑1 (MQ=17)

                    tGGCACGGCTGGGACGGAAGTCCCTGTCGTTTTCATAATCGGTGGAGCGGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAAtt                                               <  2:2212511/140‑1 (MQ=255)

                     ggCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTa                                              <  1:35023/140‑1 (MQ=255)

                     ggCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTa                                              <  1:4794129/140‑1 (MQ=255)

                     ggCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTa                                              >  1:4007501/1‑140 (MQ=255)

                      gCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGAATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTag                                             >  2:416783/1‑140 (MQ=255)

                       cACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTaga                                            <  2:5824178/140‑1 (MQ=255)

                       cACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTaga                                            <  1:3724385/140‑1 (MQ=255)

                       cACGGCTGGGACGGAAGGCGCTTTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTaga                                            <  2:2713414/140‑1 (MQ=255)

                        aCGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTagag                                           <  2:5248661/140‑1 (MQ=255)

                         cGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAgaga                                          <  2:1765995/140‑1 (MQ=255)

                         cGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAgaga                                          <  2:686709/140‑1 (MQ=255)

                          ggCTGGGACGGAAGTTGCTGTCTTTCGCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACAAAAAACCCTAGCAATAAGTGGGAGTCATAACCGACATTAAAAGTAACTTTTTTGAAATTAGAGat                                         <  1:2528477/140‑1 (MQ=17)

                          ggCTGGGACGGAAGTTGCTGTCGTTTTCAGAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGat                                         <  1:2500914/140‑1 (MQ=255)

                          ggCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGat                                         <  2:787127/140‑1 (MQ=255)

                          ggCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGat                                         <  1:3907339/140‑1 (MQ=255)

                            cTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTa                                              >  1:7365798/1‑133 (MQ=255)

                            cTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGataa                                       >  2:1651881/1‑140 (MQ=255)

                             tGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGataat                                      >  1:427899/1‑140 (MQ=255)

                             tGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGataat                                      >  2:6171825/1‑140 (MQ=255)

                              gggACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGataata                                     <  1:4368979/140‑1 (MQ=255)

                              gggACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGataata                                     <  2:139130/140‑1 (MQ=255)

                               ggACGGAAGTCGCTGTCGTTCTGAAAATCCGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAatat                                    <  1:2662228/140‑1 (MQ=255)

                                   ggAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGc                                <  1:7491074/140‑1 (MQ=255)

                                   ggAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGc                                <  2:113298/140‑1 (MQ=255)

                                     aaGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGcac                              <  1:4043297/140‑1 (MQ=255)

                                     aaGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGcac                              <  2:4637099/140‑1 (MQ=255)

                                     aaGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGcac                              <  1:1306118/140‑1 (MQ=255)

                                     aaGTCGCTGTCGTTCCCAAAATAGGTGGACCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGcac                              <  1:3253887/140‑1 (MQ=255)

                                      aGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGcaca                             >  1:286611/1‑140 (MQ=255)

                                       gTCGGTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  2:7004603/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCTAAATCGATGCAGCCGCATGACTAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:1984505/140‑1 (MQ=18)

                                       gTCGCTGTCGTTCTCAGAATCGTTGGAGCAGGAGGTCAACGTCATCGGTCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  2:3403161/140‑1 (MQ=18)

                                       gTCGCTGTCGTTCTCAAAATCGTTGGAGCAGGATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  2:2493367/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACTGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:6025454/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  2:4771297/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:1501635/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:1418638/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  2:7388096/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:1036471/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:7215571/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:6974253/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:5820776/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:5862003/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            <  1:6171825/140‑1 (MQ=255)

                                       gTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATCAGAGATAATATCGGCACAg                            <  2:4382682/140‑1 (MQ=255)

                                         cGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGaa                          <  2:4936038/140‑1 (MQ=255)

                                          gCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGcac                              >  2:6479134/1‑135 (MQ=255)

                                          gCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGaaa                         >  1:6415149/1‑140 (MQ=255)

                                          gCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGaaa                         >  1:6632431/1‑140 (MQ=255)

                                               cGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAatata                    >  2:4066034/1‑140 (MQ=255)

                                                gTTCTCAAAATCGGTGGGGCAGCATGACAAATTCAGCGGCCATTATCTAAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAatatat                   <  2:3218268/140‑1 (MQ=21)

                                                 ttCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAg                            >  2:428877/1‑130 (MQ=255)

                                                   ctcAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGca                               >  2:711001/1‑125 (MQ=255)

                                                   ctcAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCa                >  1:1749081/1‑140 (MQ=255)

                                                     cAAAATCGGTGGAGCAGTATGAAAAAGTCATAGGGTATTTTCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGt              <  2:2537081/140‑1 (MQ=25)

                                                     cAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGt              <  2:6367394/140‑1 (MQ=255)

                                                     cAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGt              <  2:6632431/140‑1 (MQ=255)

                                                     cAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGt              <  1:1083341/140‑1 (MQ=255)

                                                     cAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGt              <  1:7098549/140‑1 (MQ=255)

                                                     cAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGAATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGt              <  1:416783/140‑1 (MQ=255)

                                                            ggTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTa       <  1:5894326/140‑1 (MQ=255)

                                                            ggTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTa       >  2:5448264/1‑140 (MQ=255)

                                                             gTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAg      <  1:1148287/140‑1 (MQ=255)

                                                              tGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGt     <  1:6537641/140‑1 (MQ=255)

                                                              tGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGt     <  2:1495320/140‑1 (MQ=255)

                                                                gAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAg               <  1:1280362/128‑1 (MQ=255)

CCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGAT  >  NC_000913/3584063‑3584267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`