BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,820,757	T→C	K138K (AAA→AAG)	ligB ←	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,820,757	0	T	C	99.5%	1615.7 / NA	440	K138K (AAA→AAG)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (213/224); minor base G (0/2); total (214/226)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 450 total aligned reads displayed.

TGGAGAAATATTTCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGTTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGA  >  NC_000913/3820620‑3820892

  gagaAATATTTCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTtcc                                                                                                                                      >  1:5624259/1‑140 (MQ=255)

     aaaTATTTCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTtcccgt                                                                                                                                   <  2:6389831/140‑1 (MQ=255)

     aaaTATTTCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTtcccgt                                                                                                                                   <  2:4649781/140‑1 (MQ=255)

     aaaTATTTCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGAGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTtcccgt                                                                                                                                   <  2:4076467/140‑1 (MQ=255)

          tttCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGa                                                                                                                              <  1:4058854/140‑1 (MQ=255)

          tttCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGa                                                                                                                              <  2:1638002/140‑1 (MQ=255)

            tCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATa                                                                                                                            >  1:1427824/1‑140 (MQ=255)

            tCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATa                                                                                                                            >  1:4373606/1‑140 (MQ=255)

              ccccTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATaaa                                                                                                                          >  2:5875177/1‑140 (MQ=255)

              ccccTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATaaa                                                                                                                          <  1:5875177/140‑1 (MQ=255)

                ccTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAAcc                                                                                                                        <  2:1427824/140‑1 (MQ=255)

                ccTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAAcc                                                                                                                        <  2:4167679/140‑1 (MQ=255)

                ccTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAAcc                                                                                                                        <  1:4898130/140‑1 (MQ=255)

                  tGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAg                                                                                                                      >  2:4302481/1‑140 (MQ=255)

                   gAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGCCTGCTTGATTTGTTCATCTTCCCGTCCCGATAAACCAgg                                                                                                                     >  2:2920284/1‑140 (MQ=255)

                   gAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTtcccgt                                                                                                                                   <  2:3775562/126‑1 (MQ=255)

                   gAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAgg                                                                                                                     >  2:5975525/1‑140 (MQ=255)

                     aGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGt                                                                                                                   <  2:4849692/140‑1 (MQ=255)

                     aGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGt                                                                                                                   <  1:4858567/140‑1 (MQ=255)

                           cTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTGTGTTCAGATTCGCGGCCCGATGAACCAGGGTTACC‑Gc                                                                                                            >  1:2312506/1‑140 (MQ=255)

                              ttGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAAc                                                                                                         >  1:1488716/1‑140 (MQ=255)

                               tGGCTAAAGGCCCGCTAACGGTTTGCGGCCCAGCGGAAATTAACCTAACTTTCTGCTCCAAGCCCACGCCTTTCAGGCAATAACCGGGCCTAATTCTTTTGTCAAGCTTCCCG‑CCCAATTAAACAAGGTTACCAGCAAcc                                                                                                        >  2:6479529/1‑139 (MQ=255)

                                ggCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGc                                                                                                       >  1:3745850/1‑140 (MQ=255)

                                ggCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGc                                                                                                       >  2:6206488/1‑140 (MQ=255)

                                 gCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGcc                                                                                                      <  2:3081775/140‑1 (MQ=255)

                                   tAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCAt                                                                                                    <  1:4302481/140‑1 (MQ=255)

                                    aaaGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATc                                                                                                   >  1:1772515/1‑140 (MQ=255)

                                     aaGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCa                                                                                                  >  2:871428/1‑140 (MQ=255)

                                      aGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCaa                                                                                                 >  1:1028208/1‑140 (MQ=255)

                                      aGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCaa                                                                                                 >  1:3251541/1‑140 (MQ=255)

                                      aGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGAGTTACC‑GCAACGCCATCaa                                                                                                 >  2:4496042/1‑140 (MQ=255)

                                        gCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCa                                                                                                           <  1:5931144/128‑1 (MQ=255)

                                        gCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCAt                                                                                                    <  2:6293004/135‑1 (MQ=255)

                                        gCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACt                                                                                               >  2:7034573/1‑140 (MQ=255)

                                        gCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACt                                                                                               >  1:3395006/1‑140 (MQ=255)

                                               aaCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCt                                                                                        <  2:3985423/140‑1 (MQ=255)

                                               aaCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCt                                                                                        <  2:1772515/140‑1 (MQ=255)

                                               aaCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCt                                                                                        <  2:1488716/140‑1 (MQ=255)

                                                aCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTg                                                                                       <  2:5449016/140‑1 (MQ=255)

                                                aCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTg                                                                                       <  2:7530067/140‑1 (MQ=255)

                                                aCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTg                                                                                       <  1:6779826/140‑1 (MQ=255)

                                                aCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTg                                                                                       <  1:4229472/140‑1 (MQ=255)

                                                aCGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTg                                                                                       <  1:521722/140‑1 (MQ=255)

                                                 cGGTTTGCGGCACAGCGTAAATAAAGCTAACTTTCTGCGACCAGTCCTGGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGc                                                                                      <  2:3141134/140‑1 (MQ=255)

                                                     ttGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACttt                                                                                             <  1:670795/129‑1 (MQ=255)

                                                     ttGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAAGGTTACC‑GCAACGCCATCAACTTTTGGCTGCAccc                                                                                  <  2:6883103/140‑1 (MQ=255)

                                                      tGCGGCACAGCGGAATTTAAGCGAACTTTCTGCGTCCCGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCa                                                                                 <  1:2096378/140‑1 (MQ=255)

                                                      tGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTGCCGATAAAGCAGAGGTACC‑GCAACGGCATCAGCTTTTGGCTGCACCCa                                                                                 >  2:3345955/1‑140 (MQ=255)

                                                      tGCGACACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCa                                                                                 <  1:6410800/140‑1 (MQ=255)

                                                        cGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCaaa                                                                               <  1:6533831/140‑1 (MQ=255)

                                                        cGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCaaa                                                                               <  1:3867018/140‑1 (MQ=255)

                                                             caGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTAAC‑GCAACGCCATGAAGTTTTGGATGCACCCAAAGATCg                                                                          >  1:2939943/1‑140 (MQ=255)

                                                              aGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCAc                                                                                    >  2:7173922/1‑129 (MQ=255)

                                                              aGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGc                                                                         >  2:4920072/1‑140 (MQ=255)

                                                               gCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCt                                                                        <  2:1288034/140‑1 (MQ=255)

                                                               gCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCt                                                                        <  2:7409017/140‑1 (MQ=255)

                                                               gCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCt                                                                        <  2:3251541/140‑1 (MQ=255)

                                                               gCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCt                                                                        <  2:6761552/140‑1 (MQ=255)

                                                                cGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACGAGGGTTACG‑GCAACGCCTTCAACTTTTGGCCACACCCAAAGATCGCTa                                                                       >  2:3572680/1‑140 (MQ=255)

                                                                cGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTa                                                                       >  1:5095018/1‑140 (MQ=255)

                                                                    aaTTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCGTTGTTCAGCTTCCCGTCCCGATAAACCAGGGCTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGtt                                                                   <  2:4393243/140‑1 (MQ=255)

                                                                        aaGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCg                                                               <  1:4768633/140‑1 (MQ=255)

                                                                        aaGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCAACAACTTTTGGCAGCCCCCAAATATCGCTACGTTCTCg                                                               >  1:3140922/1‑140 (MQ=255)

                                                                            tAACTTTCTGCGTCCAGTCCTCCCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATc                                                           <  2:3395006/140‑1 (MQ=255)

                                                                            tAACTTTCTGCCTCCAGGCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCCCCCAAAGATCGCTACGTTCTCGCATc                                                           <  2:1183142/140‑1 (MQ=255)

                                                                              aCTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCTATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCca                                                         <  1:543864/140‑1 (MQ=255)

                                                                              aCTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCca                                                         <  1:732640/140‑1 (MQ=255)

                                                                              aCTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCca                                                         <  1:7125685/140‑1 (MQ=255)

                                                                               cTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCcac                                                        <  1:953867/140‑1 (MQ=255)

                                                                               cTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACACCATCAACTTTTCGCTCCACCCAAAAATCGCTACGTTCTCGCATCcac                                                        >  2:2051698/1‑140 (MQ=255)

                                                                                 ttCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAg                                                      >  2:6649014/1‑140 (MQ=255)

                                                                                        tCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCcaca                                                       <  1:6251930/132‑1 (MQ=255)

                                                                                         ccAGTCCTCGCCTTTTAGGCCACTATCGCGACTGATTTCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATg                                              <  1:2513582/140‑1 (MQ=255)

                                                                                         ccAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATg                                              >  1:5011393/1‑140 (MQ=255)

                                                                                         ccAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATg                                              >  1:7450156/1‑140 (MQ=255)

                                                                                           aGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCg                                            <  1:4473784/140‑1 (MQ=255)

                                                                                             tCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGtt                                          <  1:1277569/140‑1 (MQ=255)

                                                                                                tCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTACACCCAAAAATCACTAAGTTCTCGCGTCCAAAGACTTAATGCGttttt                                       >  2:2379908/1‑140 (MQ=255)

                                                                                                  gCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTa                                                 <  1:4533989/128‑1 (MQ=255)

                                                                                                       tCAGGCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATccacc                                >  1:6108952/1‑140 (MQ=255)

                                                                                                          ggCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCAtt                             <  1:1610351/140‑1 (MQ=255)

                                                                                                          ggCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCAtt                             <  2:5624259/140‑1 (MQ=255)

                                                                                                          ggCCATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCAtt                             <  2:387789/140‑1 (MQ=255)

                                                                                                            ccATTACCGCGACTGGTTGCTTGGTTCAGGTTCCCGTGCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCAtttt                           <  1:2687973/140‑1 (MQ=255)

                                                                                                            ccATTACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCAtttt                           <  1:4672669/140‑1 (MQ=255)

                                                                                                                tACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCg                        >  1:4031088/1‑139 (MQ=255)

                                                                                                                tACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGt                       >  2:5056835/1‑140 (MQ=255)

                                                                                                                tACCGCGACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCAGCCACAGACTTACTGGGTTTTTGTCCACCATTTTGCGt                       >  1:1956920/1‑140 (MQ=255)

                                                                                                                   cgcgACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTaca                    <  1:6957437/140‑1 (MQ=255)

                                                                                                                   cgcgACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTaca                    <  2:4576616/140‑1 (MQ=255)

                                                                                                                   cgcgACTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTaca                    <  2:5691503/140‑1 (MQ=255)

                                                                                                                   cgcgACTGATTGCTTAGTTCAGATTACCGGCCCGATAGACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTaca                    <  1:3575614/140‑1 (MQ=255)

                                                                                                                       aCTGATTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTAGCATCCACGGACGTAAGGCGTTTTTGTCCAACATTTTGCGTACACCCg                >  2:2145170/1‑140 (MQ=255)

                                                                                                                           aTTGCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATg            >  2:1077347/1‑140 (MQ=255)

                                                                                                                              gCTTTGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGc         <  1:5380311/140‑1 (MQ=255)

                                                                                                                              gCTTTGTTCAGCTTCCCATCCCTATAAACCAAGGTAACC‑TCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGc         <  2:2710337/140‑1 (MQ=255)

                                                                                                                                  ttttCAGCGTCCCGTCCAGATATACCAGGTTCACC‑ACAAAGCCATCAACTTTTGGATGCAACCAAAGATCGCTTCGTTCTCGCACCCACAGACTGAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAAcc     <  2:3638316/138‑1 (MQ=255)

                                                                                                                                  tGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAAcc     >  1:5718643/1‑140 (MQ=255)

                                                                                                                                  tGTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAAcc     <  2:5367023/140‑1 (MQ=255)

                                                                                                                                   gTTCAGCTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCATTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCg    <  2:4762072/140‑1 (MQ=255)

TGGAGAAATATTTCCCCCTGAAGCGTACTGTTGGCTAAAGGCCCGCTAACGGTTTGCGGCACAGCGGAAATTAAGCTAACTTTCTGCGTCCAGTCCTCGCCTTTCAGGCCATTACCGCGACTGATTGCTTTGTTCAGTTTCCCGTCCCGATAAACCAGGGTTACC‑GCAACGCCATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGA  >  NC_000913/3820620‑3820892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`