BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,877,329	G→C	A46G (GCC→GGC)	yidB ←	conserved protein YidB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,877,329	0	G	C	99.4%	1903.7 / ‑6.0	523	A46G (GCC→GGC)	yidB	conserved protein YidB
Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (249/269); minor base T (2/1); total (253/270)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 535 total aligned reads displayed.

CTGGAAGCTGTACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGGCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCA  >  NC_000913/3877194‑3877464

cTGGAAGCTGTACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCcccc                                                                                                                                     <  1:7049567/140‑1 (MQ=255)

  ggAAGCTGTACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTa                                                                                                                                   <  1:7483730/140‑1 (MQ=255)

    aaGCTGTACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTCTTGATTATTCAACCCGGTTGAGAGAATGCCCCCTAAg                                                                                                                                 >  1:3635708/1‑140 (MQ=255)

       cTGTACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAgccg                                                                                                                              <  1:5953680/140‑1 (MQ=255)

        tGTACTGGTATCCACGCCAAGTTTTTGCCCGGGATCGGATACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAgccgc                                                                                                                             <  2:2583122/140‑1 (MQ=255)

          tACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCa                                                                                                                           >  1:7301995/1‑140 (MQ=255)

            ttGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACt                                                                                                                         <  2:1324209/139‑1 (MQ=255)

              ggTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCAGTTGATTATTCAGCCAGGTTGAGAGAATGCGCCCTAAGCCGCCACttt                                                                                                                       >  2:3003308/1‑140 (MQ=255)

               gTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTg                                                                                                                      >  1:415640/1‑140 (MQ=255)

                 aTCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCa                                                                                                                    <  1:5126636/140‑1 (MQ=255)

                  tCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAg                                                                                                                   >  2:6564935/1‑140 (MQ=255)

                   ccACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGt                                                                                                                  <  1:399753/140‑1 (MQ=255)

                    cACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGTCCCCTAAGCCGCCACTTTGCAGtt                                                                                                                 >  2:6279306/1‑140 (MQ=255)

                    cACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGtt                                                                                                                 >  1:3789013/1‑140 (MQ=255)

                    cACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGACAATGCCGCCTAAGCCGCCACTTTGCAGtt                                                                                                                 >  1:2855859/1‑140 (MQ=255)

                    cACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGACTACTCAGCAAGGATGAAAGAATGCCCCCTAAGCCGCCACTTTGCAGtt                                                                                                                 >  2:3101108/1‑140 (MQ=255)

                       gCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGttttt                                                                                                              >  1:5691775/1‑140 (MQ=255)

                       gCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGttttt                                                                                                              <  1:6750713/140‑1 (MQ=255)

                       gCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGttttt                                                                                                              >  1:6884824/1‑140 (MQ=255)

                       gCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGtttt                                                                                                               >  1:4802362/1‑139 (MQ=255)

                       gCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCACCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGttttt                                                                                                              >  1:6495081/1‑140 (MQ=255)

                       gCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTAATCAGCCAGGTTGTGAGGATGCCCCCAAAGCTACCACTTTCCAGttttt                                                                                                              >  2:3373334/1‑140 (MQ=255)

                         cAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTGAGCCAGGTGGAGCGAAGGCCCCCTACGCCGGCACTTTGCAGTTTTTcc                                                                                                            >  2:3321065/1‑140 (MQ=255)

                           aGTTTTTGCCCGAGATCGGACCCCGTATTCGTGCCGACCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCcag                                                                                                          <  1:2240382/140‑1 (MQ=255)

                           aGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGGGAGAATGCCCCCTAAGCCGCCACTTTGTAGTTTTTCcag                                                                                                          <  1:6227648/140‑1 (MQ=255)

                            gTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCcagc                                                                                                         <  1:700510/140‑1 (MQ=255)

                            gTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCcagc                                                                                                         <  2:3823904/140‑1 (MQ=255)

                              ttttGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTGCTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCcagcag                                                                                                       <  2:552885/140‑1 (MQ=255)

                                 tGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCAc                                                                                                    <  2:1452095/140‑1 (MQ=255)

                                 tGCCCGAGATCGGACACCGCATATGAGCCCAGCGCCGGTTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCAc                                                                                                    <  1:3039636/140‑1 (MQ=255)

                                      gagaTCGGACACCGCTTTTGTGCCGTGCGCCGATTCCTGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGaa                                                                                               <  1:2477496/140‑1 (MQ=255)

                                       agaTCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAAt                                                                                              >  2:6798499/1‑140 (MQ=255)

                                        gaTCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATg                                                                                             <  1:1224758/140‑1 (MQ=255)

                                              acacCGCAGTTGTGCCACGCGCCGATTCCAGATACACGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGccc                                                                                       <  2:3501773/140‑1 (MQ=255)

                                               cacCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCt                                                                                      <  1:5378319/140‑1 (MQ=255)

                                                acCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTg                                                                                     <  2:735653/140‑1 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTGAGCCGCCAGTTTGCAGATTTTCCAGGAGCACCTGAATGCCGCCCTGct                                                                                   >  2:2720893/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCGGCCACTTTGCAGTATTTCCAGCAGCATCTGAATGGCGCCCTGct                                                                                   >  1:4652677/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCt                                                                                                  >  2:6448714/1‑125 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTg                                                                                     <  1:803501/138‑1 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGct                                                                                   >  2:437616/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGct                                                                                   >  2:4851292/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGct                                                                                   >  2:6669683/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGct                                                                                   >  2:1897513/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGct                                                                                   >  2:212335/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGct                                                                                   >  2:6939054/1‑140 (MQ=255)

                                                  cGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCAATTTGCAGGTTTTCCAGAAGCAACTGAATGCCGCCCTGct                                                                                   >  2:3151814/1‑140 (MQ=255)

                                                          tgCCGAGCGCCGATTGCAGTTGCTCGCCAATAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAAcc                                                                           <  1:3151814/140‑1 (MQ=255)

                                                          tgCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAAcc                                                                           <  2:5306815/140‑1 (MQ=255)

                                                          tgCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAAcc                                                                           <  1:958603/140‑1 (MQ=255)

                                                          tgCCGAGCGCCGATTCCAGTTGCTCCCCACTAACCGAGTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAAcc                                                                           <  1:3373334/140‑1 (MQ=255)

                                                          tgCCGAGCGCCGATTACAGTTGTTCTCCAGTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAAcc                                                                           <  1:2994019/140‑1 (MQ=255)

                                                          tgCCGAGCGCCGATTACAGTTGGTCGCCAGTAACCGACTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAAcc                                                                           <  1:2687928/140‑1 (MQ=255)

                                                            ccGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCa                                                                         >  1:1386886/1‑140 (MQ=255)

                                                            ccGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCa                                                                         >  1:215817/1‑140 (MQ=255)

                                                              gAGCGCCGATTCCAGTTTCTCGGCACTAACCTATTTATTCCCCTGTTGCTTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAAc                                                                       <  2:2212059/140‑1 (MQ=255)

                                                              gAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAAc                                                                       <  1:5470007/140‑1 (MQ=255)

                                                               aGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACt                                                                      <  1:4925214/140‑1 (MQ=255)

                                                                    cGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGTCAGGTTAAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTaaaa                                                                 >  2:4022864/1‑140 (MQ=255)

                                                                    cGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTaaaa                                                                 <  1:6072915/140‑1 (MQ=255)

                                                                    cGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTaaaa                                                                 <  2:1666175/140‑1 (MQ=255)

                                                                    cGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTaaaa                                                                 <  1:5574323/140‑1 (MQ=255)

                                                                    cGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCATCAACCCAACTTaaaa                                                                 <  1:4359489/140‑1 (MQ=255)

                                                                      aTTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCAGCTGAATGACGCGCTGCTCCTCAACCCAACTTAAAATa                                                               >  1:2013240/1‑140 (MQ=255)

                                                                         ccAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGcc                                                            <  2:5398654/140‑1 (MQ=255)

                                                                         ccAGTTGCTCGCCACGCACCGATTGGTTGTCCTGTTGGTTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGcc                                                            <  1:2726655/140‑1 (MQ=255)

                                                                              tGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGata                                                       <  2:5489111/140‑1 (MQ=255)

                                                                                cTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATAtt                                                     >  2:7149013/1‑140 (MQ=255)

                                                                                  cGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACTTGAATGCCGCCCAGATCCTCAACCCCACTTGCAATAGCCTGATATTTc                                                   >  2:3717800/1‑140 (MQ=255)

                                                                                  cGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTc                                                   >  2:6004464/1‑140 (MQ=255)

                                                                                  cGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCATGATATTTc                                                   >  2:7061893/1‑140 (MQ=255)

                                                                                      aCTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCg                                               <  2:415640/140‑1 (MQ=255)

                                                                                        tAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCa                                             >  2:6031680/1‑140 (MQ=255)

                                                                                        tAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCa                                             <  2:580829/140‑1 (MQ=255)

                                                                                         aaCCGATTGATTGCCCTGTTGAATACTCACCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCAt                                            <  1:2388763/140‑1 (MQ=255)

                                                                                              attgattgCCCTGTTGTCTACTAAGCCAGGTAGAGTGAACGCCCCCTACGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCt                                       <  2:2263219/140‑1 (MQ=255)

                                                                                               ttgattgCCCTGTTGATTACTCAGCCAGGTTGATAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCtt                                      >  1:1564950/1‑140 (MQ=255)

                                                                                               ttgattgCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCtt                                      >  2:4640083/1‑140 (MQ=255)

                                                                                                    tgCCCTGTTGATTACTCAGCCATGTTGAGTGAATGCCTCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGa                                 <  1:3672886/140‑1 (MQ=255)

                                                                                                      cccTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGaaa                               >  1:4475783/1‑140 (MQ=255)

                                                                                                        cTGTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAgg                             <  2:846679/140‑1 (MQ=255)

                                                                                                          gTTGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCa                           <  1:1730416/140‑1 (MQ=255)

                                                                                                           ttGATTACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCGGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCAc                          >  2:6205415/1‑140 (MQ=255)

                                                                                                              aTTACTCAGCCAGGTCGAGAGACTGCCCCTTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGa                       <  1:1958090/140‑1 (MQ=255)

                                                                                                                tACTCAGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGa                                 >  1:6625201/1‑128 (MQ=255)

                                                                                                                     aGCCAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTtc                >  2:1100933/1‑140 (MQ=255)

                                                                                                                      gCCAGGTTTAGAGAATCTCCCCAAAGCCGCAACTTTGCACTTTTTCCAACAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTtca               <  2:3663419/140‑1 (MQ=255)

                                                                                                                       ccAGGTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACt                  >  2:5643098/1‑136 (MQ=255)

                                                                                                                          ggTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCaa           >  2:4559952/1‑140 (MQ=255)

                                                                                                                          ggTTGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCaa           >  1:4059564/1‑140 (MQ=255)

                                                                                                                           gTTGAGAGAATGCCCCCCAAGCCGCCACTTTTCAGTTTTACCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCaaa          <  2:2918290/140‑1 (MQ=255)

                                                                                                                            ttGAGAGAATGCCCCCTTAGCCGCCACTTTGCAGTCTTTTCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCaaaa         <  2:2338259/140‑1 (MQ=255)

                                                                                                                            ttGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCaaaa         <  2:4913439/140‑1 (MQ=255)

                                                                                                                            ttGAGAGAATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCaaaa         <  1:7213329/140‑1 (MQ=255)

                                                                                                                                gagaATGCCCCCTAACCCGCCATTTTGCAGTTTTTCCTGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGcc     <  1:2562762/140‑1 (MQ=255)

                                                                                                                                 agaATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGccc    <  2:241657/140‑1 (MQ=255)

                                                                                                                                 agaATGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGccc    <  2:5056110/140‑1 (MQ=255)

                                                                                                                                   aaTGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCa  <  2:32264/140‑1 (MQ=255)

                                                                                                                                   aaTGCCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCa  <  1:1063882/140‑1 (MQ=255)

                                                                                                                                   aaTGCCCCCTAAGCCACCACTTTGCGGTTCTTCCAGCCGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCa  <  1:3272512/140‑1 (MQ=255)

CTGGAAGCTGTACTGGTATCCACGCCAAGTTTTTGCCCGAGATCGGACACCGCATTTGTGCCGAGCGCCGATTCCAGTTGCTCGCCACTAACCGATTGATTGCCCTGTTGATTACTCAGCCAGGTTGAGAGAATGGCCCCTAAGCCGCCACTTTGCAGTTTTTCCAGCAGCACCTGAATGCCGCCCTGCTCCTCAACCCAACTTAAAATAGCCTGATATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCA  >  NC_000913/3877194‑3877464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`