BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,881,817	T→C	T169A (ACC→GCC)	dnaN ←	beta sliding clamp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,881,817	0	T	C	99.1%	1277.1 / NA	348	T169A (ACC→GCC)	dnaN	beta sliding clamp
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (146/198); minor base G (3/0); total (149/199)
Fisher's exact test for biased strand distribution p-value = 7.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 395 total aligned reads displayed.

CAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTAAATTCGACTTCA  >  NC_000913/3881680‑3881950

cAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAGCAGACCGCCAGCCGTTGGCCGTCGGTTGCCACGgcgc                                                                                                                                     >  1:3035411/1‑140 (MQ=255)

cAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGgcgc                                                                                                                                     >  2:132097/1‑140 (MQ=255)

 aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGcgcg                                                                                                                                    <  1:1263351/140‑1 (MQ=255)

 aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGcgcg                                                                                                                                    >  1:3909612/1‑140 (MQ=255)

 aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGcgcg                                                                                                                                    >  1:4581829/1‑140 (MQ=255)

 aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGcgcg                                                                                                                                    <  1:4934638/140‑1 (MQ=255)

 aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGcgcg                                                                                                                                    >  2:6914741/1‑140 (MQ=255)

 aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGcgcg                                                                                                                                    <  1:1626634/140‑1 (MQ=255)

  gCGGATTGTCGCCGCCGTCGAGCATACGCATTAGTGCAACCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGgcgcgc                                                                                                                                   <  2:2780323/140‑1 (MQ=255)

  gCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGgcgcgc                                                                                                                                   >  2:351379/1‑140 (MQ=255)

  gCGGATTGTCGCCGCAGTCGAGCATCCGCCTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGgcgcgc                                                                                                                                   <  1:2204833/140‑1 (MQ=255)

           cgccgcCGTCGAGCATACGCATCAGTTCAATCACGCCTTTAC‑CGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCAc                                                                                                                         >  2:7039476/1‑140 (MQ=255)

             ccgccgTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCAcc                                                                                                                        >  2:5377421/1‑140 (MQ=255)

               gccgTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCtt                                                                                                                      <  1:574175/140‑1 (MQ=255)

               gccgTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCtt                                                                                                                      <  1:3836967/140‑1 (MQ=255)

                       gCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCa                                                                                                              <  2:6545839/140‑1 (MQ=255)

                       gCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCa                                                                                                              <  2:7339654/140‑1 (MQ=255)

                         aTACGCATCAGTTCAATCACGCTTTGACGCGGTACGAGCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCaaa                                                                                                            <  1:2587822/140‑1 (MQ=255)

                         aTACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCaaa                                                                                                            <  2:4537401/140‑1 (MQ=255)

                          tACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAAc                                                                                                           <  2:6756677/140‑1 (MQ=255)

                              cATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCa                                                                                                       <  1:1034570/140‑1 (MQ=255)

                              cATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCa                                                                                                       <  1:6690291/140‑1 (MQ=255)

                              cATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCa                                                                                                       <  1:5917800/140‑1 (MQ=255)

                              cATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCa                                                                                                       <  1:1685435/140‑1 (MQ=255)

                               aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCAt                                                                                                      <  2:5181494/140‑1 (MQ=255)

                               aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCAt                                                                                                      <  2:5910883/140‑1 (MQ=255)

                               aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCAt                                                                                                      <  2:1825016/140‑1 (MQ=255)

                               aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCAt                                                                                                      <  1:5492647/140‑1 (MQ=255)

                                tCAGTTCAATCACGCCTGTACGCGGTACGATCACCTAATCGCTTGGCACAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATa                                                                                                     <  2:1940218/140‑1 (MQ=255)

                                tCAGTTCAATCACGCCTCTACGAGGTACGATTACCTAATTGCTTGGCATAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATa                                                                                                     <  2:2041120/140‑1 (MQ=255)

                                 cAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGGCGGTTGCCGCGGGGCGAAGTTCTTCGCCTTGGGTTTCAAACAGCATAc                                                                                                    >  2:2144676/1‑140 (MQ=255)

                                 cAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGCCGGTTGCCCCGGGGCGCAGTTCTTCACCTTGCGTTTCAAACAGCATAc                                                                                                    >  2:3443399/1‑140 (MQ=255)

                                  aGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGCCGCGCAGATCTTCAGCTTCGGATTCAAACAGCATAcc                                                                                                   >  1:2466255/1‑140 (MQ=255)

                                    ttCAATCACGCCTTTTCGCGGTTCGCTCACCGAATGGTTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAt                                                                                                 <  1:2323075/140‑1 (MQ=255)

                                    ttCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAt                                                                                                 >  2:5081793/1‑140 (MQ=255)

                                      cAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAttt                                                                                               <  1:1542866/140‑1 (MQ=255)

                                       aaTCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACGGCATa                                                                                                     <  1:761042/133‑1 (MQ=255)

                                         tCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCATCTTGGGTTTCAAACAGCATACCATTTAAg                                                                                            >  1:2041120/1‑140 (MQ=255)

                                         tCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCaaa                                                                                                            >  2:401804/1‑124 (MQ=255)

                                         tCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCa                                                                                                       >  2:4942490/1‑129 (MQ=255)

                                              ccTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGGGCGCAGTTATTCCCCTACGGTTTCAAACAGGATACCATTTAAGTAATa                                                                                       >  2:3001222/1‑140 (MQ=255)

                                              ccTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCATTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATa                                                                                       >  1:5205177/1‑140 (MQ=255)

                                              ccTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATa                                                                                       >  1:6425914/1‑140 (MQ=255)

                                              ccTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATa                                                                                       >  2:5285352/1‑140 (MQ=255)

                                              ccTTTACGCGGTACGATCACCGAATAGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACAGTCGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAAGAGCATACCATTTAAGTAATa                                                                                       >  2:5119007/1‑140 (MQ=255)

                                               cTTTAC‑CGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGc                                                                                     <  1:7039476/140‑1 (MQ=255)

                                                   aCGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGaa                                                                                  >  1:862326/1‑140 (MQ=255)

                                                     gcgGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACg                                                                                <  1:6981663/140‑1 (MQ=255)

                                                        gTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGa                                                                                   >  2:5601479/1‑134 (MQ=255)

                                                         tACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt                                                                            >  1:5827640/1‑140 (MQ=255)

                                                         tACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt                                                                            >  1:4738980/1‑140 (MQ=255)

                                                           cGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACGATGTAAGTACTAGCGAACGTCCTGa                                                                          >  1:3529098/1‑140 (MQ=255)

                                                           cGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGa                                                                          <  1:5625228/140‑1 (MQ=255)

                                                           cGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGa                                                                          >  1:3978967/1‑140 (MQ=255)

                                                             aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATg                                                                        <  2:4494273/140‑1 (MQ=255)

                                                             aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATg                                                                        <  1:507430/140‑1 (MQ=255)

                                                               cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgcg                                                                      <  1:236038/140‑1 (MQ=255)

                                                               cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgcg                                                                      <  2:6588928/140‑1 (MQ=255)

                                                                aCCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgcgc                                                                     <  1:3761439/140‑1 (MQ=255)

                                                                   gAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGa                                                                          >  1:6169518/1‑132 (MQ=255)

                                                                   gAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATg                                                                        >  2:1564543/1‑134 (MQ=255)

                                                                   gAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCAt                                                                  >  1:6833030/1‑140 (MQ=255)

                                                                   gAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCAt                                                                  >  1:4317328/1‑140 (MQ=255)

                                                                    aaTGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATa                                                                 <  2:4317328/140‑1 (MQ=255)

                                                                       ggCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaa                                                              <  2:172476/140‑1 (MQ=255)

                                                                         cTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTACTAGCGAACGTCCTGATGCGCCATAGaaaa                                                            >  1:3222039/1‑140 (MQ=255)

                                                                         cTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaaa                                                            >  2:1542626/1‑140 (MQ=255)

                                                                         cTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaaa                                                            >  2:6045935/1‑140 (MQ=255)

                                                                         cTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACAATTTAAGTAATAGCGACCGTCCTGATGCGCCATAGaaaa                                                            >  2:3255384/1‑140 (MQ=255)

                                                                            ggCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTg                                                         >  2:4584639/1‑140 (MQ=255)

                                                                              cAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTggg                                                       <  1:5336405/140‑1 (MQ=255)

                                                                               aaaGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGt                                                      <  1:6045935/140‑1 (MQ=255)

                                                                                    ttGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCtt                                                 <  1:1403833/140‑1 (MQ=255)

                                                                                       aCCAATTGGCATTGAACTGACCTCCAGACGGTGGCTGTCCGTTGCCACTGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCaa                                              <  2:2329537/140‑1 (MQ=255)

                                                                                       aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTggg                                                       <  1:656384/131‑1 (MQ=255)

                                                                                       aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCaa                                              >  1:7417200/1‑140 (MQ=255)

                                                                                           aTTGGCATTGAACAGTCCGCCACACTGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAg                                          <  1:2800459/140‑1 (MQ=255)

                                                                                            ttGGCATTGAACAGACCTCCAGTCGGTGGCCTTCGATTGGCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGa                                         <  2:3704009/140‑1 (MQ=255)

                                                                                            ttGGCATTGAACAGACCGCCAGACGGTGGTCGTCGGTGGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGa                                         <  1:3454085/140‑1 (MQ=255)

                                                                                            ttGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGa                                         <  1:4080679/140‑1 (MQ=255)

                                                                                            ttGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGa                                         <  1:6329496/140‑1 (MQ=255)

                                                                                                 aTTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCt                                                  >  2:3772779/1‑126 (MQ=255)

                                                                                                   tGAACAGACCGCTAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCa                                  >  2:7509926/1‑140 (MQ=255)

                                                                                                     aaCAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCGTACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATc                                >  2:5708500/1‑140 (MQ=255)

                                                                                                      aCAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCAATAGAAAACTGAGTCGCTTCAATCAGACGCTTCATCg                               >  1:3677217/1‑140 (MQ=255)

                                                                                                          aCCGCCAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGc                           >  1:648302/1‑140 (MQ=255)

                                                                                                               cAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg                      >  2:1117616/1‑140 (MQ=255)

                                                                                                               cAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg                      >  2:3910700/1‑140 (MQ=255)

                                                                                                               cAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg                      >  2:7177456/1‑140 (MQ=255)

                                                                                                               cAGACGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg                      >  2:796897/1‑140 (MQ=255)

                                                                                                                aGACGGTGGCCGTCGCTTGCCATGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCgg                     <  1:1920173/140‑1 (MQ=255)

                                                                                                                  aCGGTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCa                   <  1:1801529/140‑1 (MQ=255)

                                                                                                                    ggTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCCGCGGCAgg                 >  2:4637723/1‑140 (MQ=255)

                                                                                                                     gTGGCCGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAggg                <  2:6407301/140‑1 (MQ=255)

                                                                                                                         ccGTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTaaa            >  2:4890259/1‑140 (MQ=255)

                                                                                                                         ccGTCGGTTGCCACGGCCCGCACTTCTTCACCTTCTGTTCCAAACAGCGTACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTaaa            <  2:2376552/140‑1 (MQ=255)

                                                                                                                           gTCGGTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTAAAtt          <  2:648302/140‑1 (MQ=255)

                                                                                                                              ggTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTGAATTCGa       <  1:514663/140‑1 (MQ=255)

                                                                                                                              ggTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGATTCATCGTAGCCTGCGGCAGGGTAAATTCGa       >  2:2200081/1‑140 (MQ=255)

                                                                                                                               gTTGCCACGGCGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTAAATTCGAc      <  1:7283134/140‑1 (MQ=255)

CAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGTAAATTCGACTTCA  >  NC_000913/3881680‑3881950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`