Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,035,253 A→G intergenic (+100/‑278) hemG → / → rrsA protoporphyrinogen oxidase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,035,2530AG99.1% 336.0 / NA 109intergenic (+100/‑278)hemG/rrsAprotoporphyrinogen oxidase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (49/59);  minor base C (1/0);  total (50/59)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

AAATCGCCCATTTAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGA  >  NC_000913/4035116‑4035303
                                                                                                                                         |                                                  
aaaTCGCCCATTTAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCg                                                  <  1:6149187/140‑1 (MQ=255)
   tCGCCCATTTAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCa                                               <  1:1295229/140‑1 (MQ=255)
        cATTTAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAacac                                          <  1:4219460/140‑1 (MQ=255)
        cATTTAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAacac                                          <  1:4966405/140‑1 (MQ=255)
        cATTTAACCGACAAACCGACGCCGACATACGCATAAATAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAacac                                          <  1:1977758/140‑1 (MQ=17)
          tttAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATGATGCGCCACCAATGACACGGAACAGCGGCAAACAcgc                                        >  1:2542268/1‑140 (MQ=255)
          tttAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACAcgc                                        >  1:7262475/1‑140 (MQ=255)
          tttAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAAGAACTCCCTTTAAAGCGACACCACTGACACGGAACAGCGGCAAACAcgc                                        >  2:2104168/1‑140 (MQ=18)
          tttAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAAGAACTCCCTATAAGGCGACACCACTGTCACGAAACAGCGGCAAACAcgc                                        >  2:2115813/1‑140 (MQ=18)
          tttAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAAAAACTCCCTGTAAGGCGACACCACTGGCACGGAACAGCGGCAAACAcgc                                        >  2:2564783/1‑140 (MQ=18)
           ttAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAGATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACAcgcc                                       >  2:1815218/1‑140 (MQ=255)
            tAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACAcgccg                                      <  2:7336008/140‑1 (MQ=255)
              aCCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACGCCCTATAATGCGACACCACTGACACGGAACACCGGCAAAAACgccgcc                                    >  2:3467689/1‑140 (MQ=255)
                cGACAAACCGACGCTTAAATAACCATAAAGAATAAAATATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCgg                                  <  1:2646029/140‑1 (MQ=255)
                cGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCgg                                  <  2:5932255/140‑1 (MQ=255)
                 gACAAACTGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCGTATAATGCACCACCACGGACACGGAACAGCGGCAAACACGCCGCCggg                                 >  2:2437124/1‑140 (MQ=17)
                 gACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCAACCACTGACACTGATCAGCGGTAAACACGCCGCCggg                                 >  1:3507597/1‑140 (MQ=18)
                   cAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTc                               >  2:7047267/1‑140 (MQ=255)
                   cAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTc                               >  2:6397812/1‑140 (MQ=255)
                    aaaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCa                              >  1:6097904/1‑140 (MQ=255)
                     aaCCGCCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAGTTTCCTCTTGTCAGGCCGGAATACCTCCCTATAATGCGCCACCTCTTACACGGAACAGCGGAAAACCCGCCGCCGGGTCAg                             <  2:1438933/140‑1 (MQ=17)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCGCTGACACGGAAAAGCGGCAAAGACGCCGCCGGGTCAg                             >  1:2657380/1‑140 (MQ=255)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGGGGCAAACACGCCGCCGGGTCAg                             >  1:3020994/1‑140 (MQ=255)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAg                             <  1:1008103/140‑1 (MQ=255)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAg                             >  2:1107918/1‑140 (MQ=255)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAg                             <  2:6988306/140‑1 (MQ=255)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAg                             <  2:4146349/140‑1 (MQ=255)
                     aaCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCACGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAg                             <  1:2719683/140‑1 (MQ=255)
                     aaCCGACGCTGAAATAACCATAAAGAATAAACAATTCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAg                             <  2:3020994/140‑1 (MQ=255)
                      aCCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGc                            >  2:3731897/1‑140 (MQ=255)
                       ccGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCg                           <  1:6400681/140‑1 (MQ=255)
                       ccGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCg                           <  1:4600760/140‑1 (MQ=255)
                       ccGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCg                           <  1:4881388/140‑1 (MQ=255)
                       ccGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCg                           <  1:4535731/140‑1 (MQ=255)
                        cGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgg                          >  1:5325226/1‑140 (MQ=255)
                         gACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCggg                         <  1:5691277/140‑1 (MQ=255)
                          aCGCTGAATTAAGCATCCAGAACAAAAAATGTGCGCTCACAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:3595688/140‑1 (MQ=14)
                          aCGCTGAAATAATCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:6976567/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATGTAGAACAAAAAATGCGGGCTCAAAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:3260591/140‑1 (MQ=14)
                          aCGCTGAAATAAGCATATAGAAAAAAAAATGCGCGCTCAGAAAATTATGTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:2818077/140‑1 (MQ=14)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGGGCGATCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:3275073/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGTTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:3197954/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        >  2:7038006/1‑140 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:1778487/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:7379259/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:5744886/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:5536790/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:231629/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:5325226/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:3921262/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  2:3777591/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  1:72324/140‑1 (MQ=255)
                          aCGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACCCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCgggg                        <  1:5754727/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAGATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  1:1815218/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCgg                                  >  2:5528466/1‑128 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCgg                                  <  1:5528466/128‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  1:41205/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  1:1304525/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  2:4468316/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  1:4910012/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  1:7230084/140‑1 (MQ=255)
                            gCTGAAATAAGCATAAACAATATAAAATGCGCGGTTAGACAATTATTTCAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      <  2:2035482/140‑1 (MQ=14)
                             cTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTtc                     >  2:5585796/1‑140 (MQ=255)
                                aaaTAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTcc                  >  1:7145507/1‑140 (MQ=255)
                                aaaTAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTcc                  >  2:7299834/1‑140 (MQ=255)
                                  aTAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACCCGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTg                <  2:4772624/140‑1 (MQ=255)
                                  aTAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTg                >  2:4976244/1‑140 (MQ=255)
                                    aaGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTgag              <  1:6363003/140‑1 (MQ=255)
                                    aaGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTgag              <  1:6324693/140‑1 (MQ=255)
                                     aGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTgaga             >  1:6496756/1‑140 (MQ=255)
                                     aGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTgaga             >  1:4392886/1‑140 (MQ=255)
                                      gCATAAAGAATAAAATATGCGCAGTAAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGaa            <  1:2517441/140‑1 (MQ=255)
                                      gCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGaa            <  2:7314729/140‑1 (MQ=255)
                                        aTAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCCAACACGCGGCCTGGTCAGCGGAGTTCTCCTGAGAACt          >  1:2674075/1‑140 (MQ=11)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGTACAGCGGCGAACACGCTGCCGGGTCAGCGGAGTTCTCCTGAGAACTcc        >  2:2136753/1‑140 (MQ=11)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAATCACGCCGGCGGTTCAGCGGGGGTCTCCTGAGAACTcc        >  1:1934066/1‑140 (MQ=11)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGTGGGGTTCTCCTGAGAACTcc        >  2:5324565/1‑140 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGtt                      >  2:3092782/1‑126 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTtct                    <  2:4777598/128‑1 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTtct                    >  1:4777598/1‑128 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTcc        >  2:4252452/1‑140 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTcc        >  1:6074912/1‑140 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTcc        >  2:6341460/1‑140 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTcc        >  1:4541281/1‑140 (MQ=25)
                                          aaaGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTcc        >  1:319081/1‑140 (MQ=25)
                                            aGAATAAAAAATGCGCGGTCAGCAATTTACTTTAAATGTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCgg      <  1:2136753/140‑1 (MQ=11)
                                            aGAATAAAAAATGCGCGGTCAGAAAATTATTTTAATTTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCgg      <  2:3281925/140‑1 (MQ=18)
                                            aGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCgg      >  1:1271477/1‑140 (MQ=18)
                                               aTAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCag   >  1:6260345/1‑140 (MQ=14)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCGGCCGGGTGAGCAGGGTTCTCCGGAGAACTCCGGCaga  >  1:2267621/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCGGCCGGGTAAGCGGGATTCTCCTGAGAACTCCGGCaga  >  1:2580103/1‑140 (MQ=2)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  >  1:4136300/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  <  1:940626/140‑1 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  >  1:5902495/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  <  1:6889389/140‑1 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  >  2:1329613/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  >  2:7256086/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  >  1:6312499/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCaga  >  1:129891/1‑140 (MQ=11)
                                                tAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAGCGGCAAACACGCCGCCGGGTAAGCAGGTTTCTCCGGAGAACTCCGGCaga  >  1:2568292/1‑140 (MQ=11)
                                                                                                                                         |                                                  
AAATCGCCCATTTAACCGACAAACCGACGCTGAAATAAGCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCACCACTGACACGGAACAACGGCAAACACGCCGCCGGGTCAGCGGGGTTCTCCTGAGAACTCCGGCAGA  >  NC_000913/4035116‑4035303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑