BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,079,837	C→T	D29D (GAC→GAT)	yiiF →	protein YiiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,079,837	0	C	T	99.8%	2134.8 / NA	619	D29D (GAC→GAT)	yiiF	protein YiiF
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (307/310); total (309/310)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 625 total aligned reads displayed.

TTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGG  >  NC_000913/4079700‑4079973

    aaaaTGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTa                                                                                                                                    >  2:5551386/1‑140 (MQ=255)

         gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGa                                                                                                                               <  2:1522219/140‑1 (MQ=255)

         gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGa                                                                                                                               <  2:4104110/140‑1 (MQ=255)

           tatTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAg                                                                                                                             <  2:740993/140‑1 (MQ=255)

              tGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCgg                                                                                                                          <  2:5445467/140‑1 (MQ=255)

               ggCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGt                                                                                                                         >  1:5027578/1‑140 (MQ=255)

               ggCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGt                                                                                                                         <  2:6092106/140‑1 (MQ=255)

               ggCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGt                                                                                                                         <  1:1481097/140‑1 (MQ=255)

                  gagaTATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAg                                                                                                                                  <  2:4813481/128‑1 (MQ=255)

                   agaTATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGACGTGCAGCGCAATTTTCGTCGTGCGGATCTAGTAAGGGAAGCGGTAGAt                                                                                                                     >  2:2849367/1‑140 (MQ=255)

                       atTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAAt                                                                                                                 <  2:122701/140‑1 (MQ=255)

                        tttATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATa                                                                                                                <  2:5084605/140‑1 (MQ=255)

                        tttATGAACTCTCTGCCAGGTATAGTTATAGGCAGAACTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATa                                                                                                                <  1:2106936/140‑1 (MQ=255)

                              aaCTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGAt                                                                                                          >  1:7411993/1‑140 (MQ=255)

                               aCTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATa                                                                                                         >  2:929032/1‑140 (MQ=255)

                               aCTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGGGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATa                                                                                                         >  1:5629088/1‑140 (MQ=255)

                                ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCgg                                                                                                                          >  1:5469344/1‑122 (MQ=255)

                                ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATaa                                                                                                        >  2:3896573/1‑140 (MQ=255)

                                 tctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATaaa                                                                                                       >  1:5276958/1‑140 (MQ=255)

                                  ctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCTGATGTATGAAGGGAAGGGGTAAATCAATACCTGATAaat                                                                                                      >  2:1948662/1‑140 (MQ=255)

                                  ctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAaat                                                                                                      >  1:1103635/1‑140 (MQ=255)

                                   tctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATa                                                                                                                >  1:6033801/1‑129 (MQ=255)

                                   tctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAaatc                                                                                                     >  1:5411174/1‑140 (MQ=255)

                                   tctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAaat                                                                                                      >  1:1592397/1‑139 (MQ=255)

                                    ctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAaatca                                                                                                    >  1:1008389/1‑140 (MQ=255)

                                          ggTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCa                                                                                              <  2:6883329/140‑1 (MQ=255)

                                           gTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaa                                                                                             <  2:7022716/140‑1 (MQ=255)

                                            tataGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATAAATACCTGATAAATCAATCGCaaa                                                                                            <  2:402038/140‑1 (MQ=255)

                                             ataGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAAc                                                                                           >  2:5615075/1‑140 (MQ=255)

                                                gATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                                            >  1:4197508/1‑136 (MQ=255)

                                                gATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGc                                                                                        <  2:424066/140‑1 (MQ=255)

                                                 atatGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCa                                                                                       >  2:1137391/1‑140 (MQ=255)

                                                     gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTATGGGAAGCGTTAGCTCACTACCTGATAAATCAATCGCAAACAGCAAGaa                                                                                   >  2:2748373/1‑140 (MQ=255)

                                                     gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATGAATCAATCGCAAACAGCAAGaa                                                                                   >  1:3492510/1‑140 (MQ=255)

                                                     gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                                   >  2:6019900/1‑140 (MQ=255)

                                                     gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                                   >  1:6436892/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAGCTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGa                                                                                    >  2:6272136/1‑138 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAATGGAAGCGGGAGATCAAGACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  2:2598598/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCa                                                                                              >  1:988425/1‑128 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCa                                                                                       >  1:697520/1‑135 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  1:5833998/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  2:1772793/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  2:5204472/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  1:2811895/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  2:6422186/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  1:7125567/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  1:4694670/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  1:3078625/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                                  >  1:4080892/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAACACCTGATACATCACTCGCAAACAGCAAGAAc                                                                                  >  2:2470183/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTACATCAATAGCTGTTATATCAATGGCAAACAGCAAGAAc                                                                                  >  1:2283603/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGAAGAGCAATACCTGATATATCACTCGCAAACAGCAAGAAc                                                                                  >  2:2412562/1‑140 (MQ=255)

                                                      ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAAGGAAGCGGTAGAACAAGACCTGATAGATCAGTCGCAAACAGCAAGAAc                                                                                  >  2:2113231/1‑140 (MQ=255)

                                                       gCAGAATTTTACTCGATTTATCCAACGAGATGATTAACCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAcc                                                                                 <  1:2010278/140‑1 (MQ=255)

                                                          gAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                                            >  1:6834641/1‑126 (MQ=255)

                                                           aaTTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCACTACCTGATAAAACACTCGCAAACAGCAAGAACCAgtg                                                                             >  1:2436246/1‑140 (MQ=255)

                                                           aaTTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                                            >  2:5162186/1‑125 (MQ=255)

                                                                tACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCt                                                                        >  2:165541/1‑140 (MQ=255)

                                                                tACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCt                                                                        <  1:4905342/140‑1 (MQ=255)

                                                                    cGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCa                                                                    <  1:1896321/140‑1 (MQ=255)

                                                                           tCGAATGAGGTGATTTAGCAACATGTTGAACTCGAGGAGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCa                                                             <  1:2748373/140‑1 (MQ=255)

                                                                            cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                            <  1:3925420/140‑1 (MQ=255)

                                                                              aaTGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAgg                                                          <  2:5276958/140‑1 (MQ=255)

                                                                              aaTGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAgg                                                          <  2:4948373/140‑1 (MQ=255)

                                                                                tGAGGTGATTAAGCAGCTTGATCATCTCGTGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgt                                                        <  1:2236041/140‑1 (MQ=255)

                                                                                tGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgt                                                        >  2:6752257/1‑140 (MQ=255)

                                                                                tGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAAACGCAAACAACAAGAACCAGGGTTCCTGGCATCTGGCAAgggg                                                        >  2:3362858/1‑139 (MQ=255)

                                                                                 gAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgtg                                                       >  1:849580/1‑140 (MQ=255)

                                                                                  aGGTGATTAAGCAACCTGATGAGCTGGAGGTGCAGCGAAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgtgt                                                      <  1:3710016/140‑1 (MQ=255)

                                                                                     tGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGAAAGAACCAGTGATCCTGGCATGTGGCAAGGGTGTGAg                                                   >  1:3221925/1‑140 (MQ=255)

                                                                                       aTTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGa                                                 <  1:6341050/140‑1 (MQ=255)

                                                                                          aaGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGa                                              <  1:1757164/140‑1 (MQ=255)

                                                                                             cAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCa                                                             <  2:6574960/122‑1 (MQ=255)

                                                                                             cAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATgg                                           >  2:5833690/1‑140 (MQ=255)

                                                                                              aaCTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCATTGTTCCTAGCACCTCGCAAGGATGTGAGGAAGATggg                                          >  1:3341522/1‑139 (MQ=255)

                                                                                                 ttGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTc                                       >  1:294388/1‑140 (MQ=255)

                                                                                                 ttGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGATCCTGGCCTCTCGCGAGGGTGTGAGGAAGATGGTGTc                                       >  1:3411177/1‑140 (MQ=255)

                                                                                                  tgatgaTCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCg                                      <  2:22/140‑1 (MQ=255)

                                                                                                      gatCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAGCAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata                                  <  2:5726623/140‑1 (MQ=255)

                                                                                                             aGGTGCAGCGTAACCTATCTCGAGCGGATCTGTAATGGGAAGCGGTAGTTCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTGCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGGCGAATATCAGCGt                           <  2:3706681/140‑1 (MQ=255)

                                                                                                                tGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAg                        <  1:5763633/140‑1 (MQ=255)

                                                                                                                tGCAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGCCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAg                        <  2:4371201/140‑1 (MQ=255)

                                                                                                                  cAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCGAGGGTGTGAGGAAGATCGTGTGGAATATCAGCGTAAGCt                      >  2:2321617/1‑140 (MQ=255)

                                                                                                                  cAGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCt                      >  2:7232563/1‑140 (MQ=255)

                                                                                                                   aGCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTg                     >  1:6269682/1‑140 (MQ=255)

                                                                                                                    gCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgc                    >  1:4494837/1‑140 (MQ=255)

                                                                                                                    gCGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgc                    <  1:5204472/140‑1 (MQ=255)

                                                                                                                     cGTAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg                   >  1:7212088/1‑140 (MQ=255)

                                                                                                                       tAATCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg                 <  1:6686439/140‑1 (MQ=255)

                                                                                                                       tAATCTTCCTCGTGCGGATCTATTAAGGGAAACCGTAGATCAATACCTTATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg                 <  2:3492510/140‑1 (MQ=255)

                                                                                                                         aTCTTCCTCGTGCGGATCTATTCAGGGAAGCAGTATATCAATACCTGACAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAg               <  2:3023627/140‑1 (MQ=255)

                                                                                                                          tCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg                 >  1:2485388/1‑137 (MQ=255)

                                                                                                                          tCTTCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAgg              >  2:470068/1‑140 (MQ=255)

                                                                                                                            ttCCTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGaa            >  1:4622869/1‑140 (MQ=255)

                                                                                                                               cTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATgg         >  1:5546861/1‑140 (MQ=255)

                                                                                                                               cTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATgg         >  2:7555726/1‑140 (MQ=255)

                                                                                                                               cTCGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATAGTGACGAATATCGGCGTAAGCTGCGCGAGGAATgg         >  2:3172460/1‑140 (MQ=255)

                                                                                                                                 cGTGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATTGTGTCGAATATAAGCGTAAGCTGCGCGAGGAATGGTa       >  1:2639466/1‑140 (MQ=255)

                                                                                                                                   tGCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg                 <  1:6529066/128‑1 (MQ=255)

                                                                                                                                    gCGGATCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATg    >  1:5142320/1‑140 (MQ=255)

TTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGG  >  NC_000913/4079700‑4079973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`