Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,309,020 C→T L150L (CTG→CTA)  alsC ← D‑allose ABC transporter membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,309,0200CT99.0% 1041.4 / ‑1.3 304L150L (CTG→CTA) alsCD‑allose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (162/139);  total (163/141)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 311 total aligned reads displayed.

CGTTGTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAA  >  NC_000913/4308885‑4309157
                                                                                                                                       |                                                                                                                                         
cgTTGTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCGAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACGGAGTTGGCAGCGGAGATCACTAGCg                                                                                                                                       >  1:3097636/1‑140 (MQ=255)
cgTTGTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCg                                                                                                                                       >  1:6407009/1‑140 (MQ=255)
cgTTGTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCg                                                                                                                                       >  1:6044434/1‑140 (MQ=255)
  ttGTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTg                                                                                                                                     >  1:3939340/1‑140 (MQ=255)
    gTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGAt                                                                                                                                   >  2:1579074/1‑140 (MQ=255)
       aGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATccc                                                                                                                                >  1:3972511/1‑140 (MQ=255)
        gAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCA‑CAGCGTGATCCCAc                                                                                                                              <  1:259240/140‑1 (MQ=255)
         aaaCCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCAc                                                                                                                              <  2:4471161/140‑1 (MQ=255)
           aCCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACgg                                                                                                                            >  1:1361476/1‑140 (MQ=255)
           aCCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACgg                                                                                                                            <  2:7303625/140‑1 (MQ=255)
               aaGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGGCGTATACCCAGTAGGCATCGGAGATGACTAGCGTGATCCCACGGaaaa                                                                                                                        >  2:2494676/1‑140 (MQ=255)
                    tGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATcgcg                                                                                                                   >  1:190905/1‑140 (MQ=255)
                      aGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATAGCGAGTTGGAATCTGAGCTCACTAGCGTGATCCCACGGAAAATCGCGtt                                                                                                                 >  2:2109547/1‑140 (MQ=255)
                      aGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGtt                                                                                                                 >  2:5715436/1‑140 (MQ=255)
                      aGCGCGACAATTAGTGAAAAGAGAACGGGGACAGGAATCGCAATTACGATGGCGGCAAAGAAGTTCACTAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGtt                                                                                                                 <  2:2257974/140‑1 (MQ=255)
                       gcgcGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGtt                                                                                                                 >  1:936465/1‑139 (MQ=255)
                       gcgcGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTg                                                                                                                >  2:7555007/1‑140 (MQ=255)
                         gcgACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGt                                                                                                              <  1:1711406/140‑1 (MQ=255)
                            aCAATTAGTGAGAAGTTAACGGCGAAAGGTATCCCAAATACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGcc                                                                                                           <  1:3602367/140‑1 (MQ=255)
                            aCAATTAGTGAGAAGATGACGGTGACAGGTATCCCAATTGCGCTGGCGGCGAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGcc                                                                                                           <  2:3097636/140‑1 (MQ=255)
                            aCAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGcc                                                                                                           <  1:5227533/140‑1 (MQ=255)
                              aaTTAGTGAGAAGATTACGGGGACATGTACCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCaa                                                                                                         <  1:1947018/140‑1 (MQ=255)
                                ttAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAgg                                                                                                       <  1:1143315/140‑1 (MQ=255)
                                  aGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGtt                                                                                                                 >  1:7442810/1‑128 (MQ=255)
                                  aGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGtt                                                                                                                 >  1:1036321/1‑128 (MQ=255)
                                  aGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGcc                                                                                                           >  1:3848645/1‑134 (MQ=255)
                                  aGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGcc                                                                                                           <  2:3848645/134‑1 (MQ=255)
                                    tGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGtga                                                                                                   <  2:6577903/140‑1 (MQ=255)
                                    tGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGtga                                                                                                   <  1:4155227/140‑1 (MQ=255)
                                       gaagaTAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGtgatga                                                                                                <  1:3180539/140‑1 (MQ=255)
                                       gaagaTAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCACGCTGGTATCAAGTGtgttga                                                                                                >  2:6997579/1‑140 (MQ=255)
                                           aTAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCGCACGGAAGATCTCGGTGGTGCGAAGGGTGATGATGaa                                                                                            >  1:2113426/1‑140 (MQ=255)
                                           aTAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGtgatga                                                                                                <  1:5716536/136‑1 (MQ=255)
                                            tAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGGGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAAc                                                                                           <  1:5958933/140‑1 (MQ=255)
                                            tAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAAc                                                                                           <  1:6756556/140‑1 (MQ=255)
                                            tAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAAc                                                                                           <  1:5599321/140‑1 (MQ=255)
                                            tAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAAc                                                                                           <  2:1472671/140‑1 (MQ=255)
                                            tAACGGGGACAGGTATCCCAATTACGCTGGCAGCAAAGATGTTCACGAGGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAAc                                                                                           <  2:2934400/140‑1 (MQ=255)
                                             aaCGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCGCGGAAAAGCGCCTTGGTGCCAAGGGTGATGATGAACg                                                                                          >  1:2383710/1‑140 (MQ=255)
                                             aaCGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAAGCGCGTTGGTGCCACGGGTGATGATGAACg                                                                                          >  1:2961957/1‑140 (MQ=255)
                                              aCGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAAc                                                                                           >  2:3951311/1‑138 (MQ=255)
                                              aCGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCAAGGAAAATCGCGCTGCTGCCAACGGTGATGATGAACgg                                                                                         >  1:3525934/1‑140 (MQ=255)
                                                  ggACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGtga                                                                                                   >  1:1726899/1‑126 (MQ=255)
                                                  ggACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGgtgt                                                                                     >  1:501989/1‑140 (MQ=255)
                                                     cAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGATGCAAACGGTGATCATGAACGGGTGTAGc                                                                                  >  1:2832890/1‑140 (MQ=255)
                                                     cAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAAGCGCGTTGGTGCGAATGGTGATAATGAACGGGTGTAGc                                                                                  >  1:2267354/1‑140 (MQ=255)
                                                      aGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcc                                                                                 <  1:1579074/140‑1 (MQ=255)
                                                      aGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcc                                                                                 <  2:4764675/140‑1 (MQ=255)
                                                       ggTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGccc                                                                                >  1:7258737/1‑140 (MQ=255)
                                                          aTCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGt                                                                             >  1:4279309/1‑140 (MQ=255)
                                                          aTCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGt                                                                             >  2:5594362/1‑140 (MQ=255)
                                                           tCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTc                                                                            >  1:10600/1‑140 (MQ=255)
                                                             ccAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCa                                                                          <  2:1056656/140‑1 (MQ=255)
                                                              cAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAg                                                                         <  2:3263923/140‑1 (MQ=255)
                                                                aTTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGtt                                                                       >  1:7515609/1‑140 (MQ=255)
                                                                  tACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGa                                                                     >  1:4548779/1‑140 (MQ=255)
                                                                   aCGCTGGCTGCAAAGACTTTCATGAAGTCAACTTACAAGACGTATACCTAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGAc                                                                    <  2:3605804/140‑1 (MQ=255)
                                                                      cTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAg                                                                 >  2:262089/1‑140 (MQ=255)
                                                                       tGGCGGCAGAGAAGTTCACGAATTCAAATGAGAAGCCGTGTACCGAGTCGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                <  2:3354316/140‑1 (MQ=255)
                                                                       tGGCGGCAAAGAAGTTCCCGAACTCAAATGAGAAGTCGTGTACCGAGTGGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                <  2:2165579/140‑1 (MQ=255)
                                                                       tGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGTATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                <  2:417780/140‑1 (MQ=255)
                                                                       tGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                >  2:682076/1‑140 (MQ=255)
                                                                       tGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                <  1:5349956/140‑1 (MQ=255)
                                                                       tGGCGGCAAAGAAGTGCACGAACTCTAATGAGAAGCCCTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                <  1:3570520/140‑1 (MQ=255)
                                                                       tGGCGGCAAAGAAGTCCACGAAATCTAATTAGAAGCCATATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAgg                                                                <  1:3035133/140‑1 (MQ=255)
                                                                         gcggcAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCa                                                              >  2:516436/1‑140 (MQ=255)
                                                                         gcggcAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCa                                                              >  2:4335589/1‑140 (MQ=255)
                                                                          cggcAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAg                                                             >  2:1258029/1‑140 (MQ=255)
                                                                                agaagTTCTCGAAGTCTAATGATAAGCCGTATATCTAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTg                                                       <  2:3489840/140‑1 (MQ=255)
                                                                                  aagTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGAt                                                     <  2:4678963/140‑1 (MQ=255)
                                                                                       cACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGccc                                                >  2:6034421/1‑140 (MQ=255)
                                                                                       cACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGccc                                                >  1:7317076/1‑140 (MQ=255)
                                                                                          gAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCa                                             >  2:639315/1‑140 (MQ=255)
                                                                                             gTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTg                                          <  2:7258737/140‑1 (MQ=255)
                                                                                              tCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTgc                                         >  1:4834052/1‑140 (MQ=255)
                                                                                                  aTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCTGGCAGCCGTTGATCGCCCCCAGTGcgccg                                     <  1:6997579/140‑1 (MQ=255)
                                                                                                  aTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGcgccg                                     <  2:1717760/140‑1 (MQ=255)
                                                                                                    gagaAGCCGTATACCTAGTTGGCATAGGATATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCgccgcc                                   <  1:3488855/140‑1 (MQ=255)
                                                                                                    gagaAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCgccgcc                                   <  2:6723622/140‑1 (MQ=255)
                                                                                                    gagaAGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCgccgcc                                   <  2:7358032/140‑1 (MQ=255)
                                                                                                       aaGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAGATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCACGGCGCCGCCAAc                                >  2:1801454/1‑140 (MQ=255)
                                                                                                       aaGCCGTACCCCGAGTTGGCATCGGAGATCAGTAGCGTGATCCCACGGGAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAAc                                <  2:3574018/140‑1 (MQ=255)
                                                                                                        aGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTg                                          <  2:4964011/129‑1 (MQ=255)
                                                                                                        aGCCGTATACCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAAcc                               >  1:4187246/1‑140 (MQ=255)
                                                                                                             tataCCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTTATCGCCACCAGTGAGCCGCCAACCAGTac                          >  1:2490239/1‑140 (MQ=255)
                                                                                                              ataCCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTaca                         >  2:4086755/1‑140 (MQ=255)
                                                                                                              ataCCGAGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTaca                         >  2:5890660/1‑140 (MQ=255)
                                                                                                                    aGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCa                   >  1:6299528/1‑140 (MQ=255)
                                                                                                                    aGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCa                   >  1:6873675/1‑140 (MQ=255)
                                                                                                                    aGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTGGATCGCCCCCAGTGCGCCGCCCACCAGTACACCGCCa                   >  1:3340347/1‑140 (MQ=255)
                                                                                                                    aGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAAGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCa                   >  1:441929/1‑140 (MQ=255)
                                                                                                                    aGTTGGCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACAAGGCAGCCGTTGATCGCCCCCAGTGAGCCGCCAACCAGTACACCGCCa                   >  2:2399254/1‑140 (MQ=255)
                                                                                                                    aGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCa                   >  2:6060989/1‑140 (MQ=255)
                                                                                                                        ggCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGGAGCCGTTGCTCGCCCCCAGTGCGCCCCCAAGCAGTACACCGCCAatca               >  2:3029720/1‑140 (MQ=255)
                                                                                                                         gCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAAc                                <  1:6607075/122‑1 (MQ=255)
                                                                                                                         gCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAatcat              >  1:842227/1‑140 (MQ=255)
                                                                                                                         gCATCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCACCCCCAGGGCGGCGACAACCATTACACCGCCAatcat              >  1:2227205/1‑140 (MQ=255)
                                                                                                                           aTCGGAGATCACTAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCg            >  2:6205886/1‑140 (MQ=255)
                                                                                                                              ggAGATCACTCGTGTGAACCCACGGAAAATCCAGTTTGGGCCCAGGGTGATAATGAACGGGTGTAACCCCGACCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTg         <  2:5110838/140‑1 (MQ=255)
                                                                                                                                ggaTCACTCGCGTCATCCTACGGAAAATCGCGTTGATGACAAGGGTGATGATGAACGGGTGTAGCCCCGACCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAGCCAATACTCCGCCAATCATCGCTGCg       <  2:5344869/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
CGTTGTCAGAAACCAAAGGATGAGCGCGACAATTAGTGAGAAGATAACGGGGACAGGTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAA  >  NC_000913/4308885‑4309157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑