BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,218	C→T	A199A (GCG→GCA)	hcp ←	protein S‑nitrosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,218	0	C	T	99.7%	1010.9 / NA	309	A199A (GCG→GCA)	hcp	protein S‑nitrosylase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (124/184); total (125/184)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

GAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACA  >  NC_000913/913082‑913354

gAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGtgcg                                                                                                                                       <  1:4642552/140‑1 (MQ=255)

 aaTGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAAAACTCAAGAAGAGCGt                                                                                                                                      >  2:6752561/1‑140 (MQ=255)

    gCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCa                                                                                                                                   >  2:6555931/1‑140 (MQ=255)

     cATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAGCACTCAAGAAGTGCGTTCat                                                                                                                                  <  1:1692435/140‑1 (MQ=255)

     cATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCat                                                                                                                                  >  2:5775547/1‑140 (MQ=255)

      aTTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGtgcg                                                                                                                                       >  1:681234/1‑134 (MQ=255)

      aTTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCata                                                                                                                                 >  1:7337520/1‑140 (MQ=255)

      aTTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCata                                                                                                                                 >  2:4979255/1‑140 (MQ=255)

           cccGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTGTTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGc                                                                                                                            >  1:1111300/1‑140 (MQ=255)

           cccGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGc                                                                                                                            >  2:4410737/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCCTAGCACACTCAACAAGTGAGTTCATATCGGCAGGc                                                                                                                        >  2:3252166/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGtgcg                                                                                                                                       <  2:5661312/125‑1 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGtgcg                                                                                                                                       <  2:1784899/125‑1 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGtgcg                                                                                                                                       >  1:5661312/1‑125 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATcggccggc                                                                                                                        >  1:4137039/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGc                                                                                                                        >  2:7037146/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGc                                                                                                                        >  1:1548284/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGc                                                                                                                        >  2:103527/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGc                                                                                                                        >  1:6849714/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGc                                                                                                                        >  1:4470905/1‑140 (MQ=255)

               ccgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGACCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGc                                                                                                                        >  2:7403226/1‑140 (MQ=255)

                cgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATTCTCATCACTTTGAAGTTCATCTGGCCGATTGCCAATGAACACTCAAGAAGCGGGTTCATATCGGCAGGcc                                                                                                                       >  2:3413737/1‑140 (MQ=255)

                cgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGcc                                                                                                                       <  2:1070329/140‑1 (MQ=255)

                cgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGcc                                                                                                                       <  2:876534/140‑1 (MQ=255)

                cgTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGcc                                                                                                                       <  2:1153533/140‑1 (MQ=255)

                cgTCGCTTTGACGTTGACCTGGGTTGGCGTCCGGTTACCCTATTTACCCGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGcc                                                                                                                       <  2:2198850/140‑1 (MQ=255)

                 gTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGTCCGCATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCa                                                                                                                      <  2:3104452/140‑1 (MQ=255)

                 gTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCa                                                                                                                      <  2:45850/140‑1 (MQ=255)

                  tCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAg                                                                                                                     <  2:1477890/140‑1 (MQ=255)

                  tCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAg                                                                                                                     >  1:583645/1‑140 (MQ=255)

                           cGTTGACCTGGGTTGGCTTCGGGTGACCGTACTTACCGGCTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGTAGGCCAGGTCCCCAGc                                                                                                            <  2:2434675/140‑1 (MQ=255)

                           cGTTGACCTGGGTTGGCGTCGGGTGGCCGGATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGc                                                                                                            <  1:2734616/140‑1 (MQ=255)

                           cGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGc                                                                                                            <  1:4731536/140‑1 (MQ=255)

                            gTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGGCAGGTCCCCAGcc                                                                                                           >  2:6665440/1‑140 (MQ=255)

                               gACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACg                                                                                                        <  1:4301580/140‑1 (MQ=255)

                                cccTGGGTTGGCGTCCGGTGACAGTATTTCCCGGTTTAGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGc                                                                                                       <  1:2297956/139‑1 (MQ=255)

                                  cTGGGTTGGCGTCGGGTTACCGTATTTACCGGTTTTGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCa                                                                                                     <  2:2922954/140‑1 (MQ=255)

                                  cTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCa                                                                                                     <  1:1911682/140‑1 (MQ=255)

                                    gggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATg                                                                                                   >  1:903231/1‑140 (MQ=255)

                                     ggTTGGCGTCGGGTGCCCGTATGTACCGGATTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  1:2978740/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  1:6665440/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  1:290183/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  2:1910270/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  1:7488370/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  2:4161802/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGa                                                                                                  <  2:4202376/140‑1 (MQ=255)

                                     ggTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACGCAAGAAGTACGTACATCACGGCAGGGCAGGGACCCAGCCTCGCCATGa                                                                                                  >  2:3612257/1‑140 (MQ=255)

                                          gCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCAt                                                                                                    <  2:1221230/133‑1 (MQ=255)

                                          gCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTa                                                                                             <  2:4669793/140‑1 (MQ=255)

                                               gggTGACCGTATTTAGCGGTTTCGCCTGCTTCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTa                                                                                        <  1:2415129/140‑1 (MQ=255)

                                               gggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCTTATCGGCAGGCAAGGTCCCCAGCCACGCCATGATTTTATGGTa                                                                                        <  2:3954836/140‑1 (MQ=255)

                                               gggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTa                                                                                        >  2:4070289/1‑140 (MQ=255)

                                               gggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTa                                                                                        <  2:583645/140‑1 (MQ=255)

                                               gggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTa                                                                                        >  1:4916050/1‑140 (MQ=255)

                                                ggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAATACTCAAGAAGTGCGTTCATATCAGCAGGCCAGGTCCCCATCCACACCATGATTTTATGGTAc                                                                                       >  2:2657881/1‑140 (MQ=255)

                                                ggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTAc                                                                                       <  1:5525739/140‑1 (MQ=255)

                                                ggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTAc                                                                                       <  1:7148281/140‑1 (MQ=255)

                                                ggTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTAc                                                                                       >  1:3793236/1‑140 (MQ=255)

                                                  tGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTg                                                                                     <  1:6015087/140‑1 (MQ=255)

                                                    aCCGTATTTACCGGTTTCGCCTTCATCCAGAGTGCTCATCACTTTGAATTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTggg                                                                                   <  2:2506610/140‑1 (MQ=255)

                                                    aCCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTggg                                                                                   <  1:1419753/140‑1 (MQ=255)

                                                    aCCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTggg                                                                                   <  2:6002667/140‑1 (MQ=255)

                                                           ttACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGATCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAat                                                                            >  2:4898538/1‑140 (MQ=255)

                                                            tACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAata                                                                           <  1:3934814/140‑1 (MQ=255)

                                                             aCCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAatat                                                                          <  2:3866122/140‑1 (MQ=255)

                                                               cGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCATCCACGCCTTGACTTAATGGTAGTGGGCATAAATATCg                                                                        >  1:3663347/1‑140 (MQ=255)

                                                               cGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCAt                                                                                >  2:6238705/1‑132 (MQ=255)

                                                               cGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCAt                                                                                <  1:6238705/132‑1 (MQ=255)

                                                                 gTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGtt                                                                      <  1:103527/140‑1 (MQ=255)

                                                                  tttCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTg                                                                     >  1:6295656/1‑140 (MQ=255)

                                                                   ttCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGt                                                                    <  2:941592/140‑1 (MQ=255)

                                                                    tCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTc                                                                   >  1:5183616/1‑140 (MQ=255)

                                                                    tCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTc                                                                   >  2:595913/1‑140 (MQ=255)

                                                                           cATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGACTTTATGGCACTAGGCATAAATTTCGTTGTCGTATTGa                                                            >  1:2388255/1‑140 (MQ=255)

                                                                                     gCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATTAATGTCATTGTCGCATTGACCGAGAACAt                                                  >  1:3511146/1‑140 (MQ=255)

                                                                                     gCTCATCACTTTGAAATTCATCAGGACGAATTCCATTTAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACAt                                                  <  1:2699367/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  2:7337520/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  2:6849714/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              >  2:6402181/1‑140 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  1:5685886/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  1:5918481/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  2:5509231/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  2:5236430/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  2:7568374/140‑1 (MQ=255)

                                                                                         atcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATgcgc                                              <  1:6559390/140‑1 (MQ=255)

                                                                                          tcaCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGCGCAGAAAGATCGTTGTGGTATGGACCGAGAACATGcgcg                                             >  2:2005604/1‑140 (MQ=255)

                                                                                           caCTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCgt                                            <  1:7037146/140‑1 (MQ=255)

                                                                                             cTTTGAAGTTCATCTGGCCGATCTCCATTTAACACTCCAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCgtgt                                          <  1:2507246/140‑1 (MQ=255)

                                                                                                tGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAAGATCGTTGCCGTCTTGACCGAGAACATGCGCGTGTTcc                                       >  1:2022136/1‑140 (MQ=255)

                                                                                                       cATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAgg                                >  2:5360879/1‑140 (MQ=255)

                                                                                                       cATCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCACAAATATCGTTGTAGTATTGACCGAGGACATACGCGTGTTCCATATAgc                                >  2:2384327/1‑139 (MQ=255)

                                                                                                        aTCTGGCCGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGgc                               >  2:7092951/1‑140 (MQ=255)

                                                                                                               cGATTTCCATTGAACTCTCAAGGAGTGCGCTCATATCCGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCa                        <  1:3252166/140‑1 (MQ=255)

                                                                                                               cGATTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCa                        <  1:812023/140‑1 (MQ=255)

                                                                                                                 aTTTCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCAcc                      >  2:1354202/1‑140 (MQ=255)

                                                                                                                    tCCATTGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCttt                   <  2:1537503/140‑1 (MQ=255)

                                                                                                                         tGAACACTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGc              <  1:5360879/140‑1 (MQ=255)

                                                                                                                         agacagcTCAAGAAGTTGGTTCGTATCGGCCGGCCTGGTCCCCAGCCATGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGc              <  2:3546565/134‑1 (MQ=255)

                                                                                                                             cacTCAAGAAGTGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATa          <  1:1264815/140‑1 (MQ=255)

                                                                                                                                tCAAGAAGTGCGTTCATATCGGCAGGCCAGGCCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAg       >  1:6497606/1‑140 (MQ=255)

GAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGTATTTACCGGTTTCGCCTGCATCCAGAATGCTCATCACTTTGAAGTTCATCTGGCCGATTTCCATTGAACACTCAAGAAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACA  >  NC_000913/913082‑913354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`