BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	204,549	A→G	G19G (GGA→GGG)	rnhB →	RNase HII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	204,549	0	A	G	99.5%	1312.0 / NA	373	G19G (GGA→GGG)	rnhB	RNase HII
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (202/168); total (203/170)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

CGATACCTTCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAG  >  NC_000913/204412‑204685

  aTACCTTCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGcgcg                                                                                                                                      <  1:4444229/140‑1 (MQ=255)

    aCCTTCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCggg                                                                                                                                    <  1:4734219/140‑1 (MQ=255)

      cTTCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGcc                                                                                                                                  >  1:7561155/1‑140 (MQ=255)

        tCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTTGATGAAGTCGGGCGCGGGCCGt                                                                                                                                >  1:3440062/1‑140 (MQ=255)

        tCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGt                                                                                                                                >  2:7370353/1‑140 (MQ=255)

        tCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGt                                                                                                                                >  2:4497982/1‑140 (MQ=255)

         ccGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGcgcg                                                                                                                                      <  2:4618215/133‑1 (MQ=255)

         ccGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGtt                                                                                                                               >  2:911529/1‑140 (MQ=255)

         ccGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGtt                                                                                                                               >  2:5770782/1‑140 (MQ=255)

          tgTGAGCTGCATTAGCAGATCCGCTGCAATGCCGATGCTCAGGCGGCACAAGCCTTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTa                                                                                                                              >  2:7541111/2‑140 (MQ=255)

           gTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAg                                                                                                                             >  2:1796436/1‑140 (MQ=255)

             gAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCCCAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGcgcg                                                                                                                                      <  1:4329532/129‑1 (MQ=255)

               aCTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTgg                                                                                                                         >  1:6458414/1‑140 (MQ=255)

               aCTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTgg                                                                                                                         >  1:2834611/1‑140 (MQ=255)

                   cATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAACCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGcgcg                                                                                                                     >  2:306084/1‑140 (MQ=255)

                   cATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGACACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGTTGTGGATAAAGCCGCGCGCGGACCGTTAGTTGGcgcg                                                                                                                     >  1:2990538/1‑140 (MQ=255)

                   cATCAGAAGATCCGCTGAAAGGCCGAGGAGCGGGGGGCAAAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGcgcg                                                                                                                     <  2:6642336/140‑1 (MQ=255)

                   cATCAAGAGATCCGCTGCAATGCCGATGATCGGGCGGCGCAAGCCGTTCTGGAGATAAGTCGATGATCGCATTTGTTTATCCGCACACGCCCATGGTTGCGGGTGTAACTGAAGTCGGATCCGAGCCGTTAGTTCGggcg                                                                                                                     >  2:5855542/1‑140 (MQ=255)

                        gcagATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGgtcgt                                                                                                                >  2:4889152/1‑140 (MQ=255)

                         cagATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGgtcgtc                                                                                                               >  1:6904898/1‑140 (MQ=255)

                           gATCCGCTGCAATGCCCGTGAGCAGGCGGCACAAGCCGTACTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCAc                                                                                                             <  2:3280253/140‑1 (MQ=255)

                             tCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCg                                                                                                           >  1:5273642/1‑140 (MQ=255)

                             tCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCCTTAGTTGGCGCGGTCGTCACCg                                                                                                           >  1:2671816/1‑140 (MQ=255)

                             tCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATAAAGACGGACGCGGGCCGTTAGTTGGCGCGGTCGTCACCg                                                                                                           >  2:2795856/1‑140 (MQ=255)

                              ccGCTGCAATGCCGAGGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGc                                                                                                          <  1:2946452/140‑1 (MQ=255)

                                 cTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGc                                                                                                       >  2:5358103/1‑140 (MQ=255)

                                 cTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGGGGATGAAGTCGGGCGCAGGCCGTTAATTGGTTCGGTCGGCACCGCTGc                                                                                                       >  2:3437015/1‑140 (MQ=255)

                                   gaaaTGCCGATGAGCAGTCGGCACAAGCCGTCCTGAAGTAAGCACAATAATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCgg                                                                                                     <  2:2521166/138‑1 (MQ=255)

                                   gCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCgg                                                                                                     <  2:4001145/140‑1 (MQ=255)

                                   gCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGCGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCgg                                                                                                     >  1:5729601/1‑140 (MQ=255)

                                    cAATGCGGATGAGCAGAGGGCAGAAGCCGTTTTAGAGTTCGCACAATGGTCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGt                                                                                                    <  2:3343379/140‑1 (MQ=255)

                                    cAATGCCGATGAGCATGCGGCACAATCCGCTCTGGAGGTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGt                                                                                                    <  1:3128521/140‑1 (MQ=255)

                                    cAATGCCGATGAGCAGGCGGCAGAAGCCGTTTTGGAGTTCGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGt                                                                                                    <  2:2723346/140‑1 (MQ=255)

                                    cAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGt                                                                                                    <  1:5377930/140‑1 (MQ=255)

                                       tGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAt                                                                                                 >  2:1874073/1‑140 (MQ=255)

                                            aTGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTg                                                                                            >  1:7181742/1‑140 (MQ=255)

                                                   ggcggcACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGgc                                                                                     >  1:1795334/1‑140 (MQ=255)

                                                   ggcggcACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGgc                                                                                     >  2:5180947/1‑140 (MQ=255)

                                                         acaAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTTGTCACCGCTGCGGTGATCCTTGACCCGGCGCGccc                                                                               >  2:1444911/1‑140 (MQ=255)

                                                         acaAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGccc                                                                               >  1:3796504/1‑140 (MQ=255)

                                                               cGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGc                                                                         >  1:1648238/1‑140 (MQ=255)

                                                                   cTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCTCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCggg                                                                     <  2:6539009/140‑1 (MQ=255)

                                                                   cTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCggg                                                                     <  1:6495225/140‑1 (MQ=255)

                                                                        gTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGaa                                                                <  2:6458414/140‑1 (MQ=255)

                                                                           aGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGa                                                             >  1:5482645/1‑140 (MQ=255)

                                                                           aGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGa                                                             >  2:1590628/1‑140 (MQ=255)

                                                                           aGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGa                                                             >  2:749533/1‑140 (MQ=255)

                                                                                  tGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGa                                                                 >  2:3770848/1‑129 (MQ=255)

                                                                                  tGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGAt                                                            >  1:6294314/1‑134 (MQ=255)

                                                                                  tGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCaaa                                                      >  1:1040705/1‑140 (MQ=255)

                                                                                     tCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAg                                                   >  2:1201619/1‑140 (MQ=255)

                                                                                     tCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAg                                                   >  2:626417/1‑140 (MQ=255)

                                                                                     tCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGAGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGAATCCAAAAAg                                                   >  2:4360633/1‑140 (MQ=255)

                                                                                      cGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCTGATTGCTGGGCTGAATGATTCCAAAAAGc                                                  >  1:3667648/1‑140 (MQ=255)

                                                                                          tttGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGAGCCCGATTGCCGGGATGAATGTTTCCAAAAAGCTGAg                                              >  1:2052592/1‑140 (MQ=255)

                                                                                          tttGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGCGGATCCTTGACCCGGCGCGCCCGATTGCGGGGCTGAATGTTTCCAGAAAGCTGAg                                              >  2:4583843/1‑140 (MQ=255)

                                                                                            tGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCg                                            >  2:5570341/1‑140 (MQ=255)

                                                                                              tttATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGaa                                          >  1:6147927/1‑140 (MQ=255)

                                                                                                   ccGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATGCCAAAAAGCTGAGCGAAAAACg                                     >  1:2194783/1‑140 (MQ=255)

                                                                                                   ccGCACACGCAGCTGGTCGCGGGTGTGGATGCAGTTGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACg                                     <  2:2052592/140‑1 (MQ=255)

                                                                                                      cacaCGCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCg                                  >  1:6841588/1‑140 (MQ=255)

                                                                                                      cacaCGCAGCTGGTTGCGGGTGTCGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCg                                  >  1:7230397/1‑140 (MQ=255)

                                                                                                           gCAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTgg                             >  1:1503758/1‑140 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGTATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  2:7081433/140‑1 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTTGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  1:1444911/140‑1 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  1:1874073/140‑1 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  1:4609138/140‑1 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  2:1402294/140‑1 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCACCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  1:6059860/140‑1 (MQ=255)

                                                                                                            cAGCTGGTTGCGGGTGTGGATGAAGTCGGGCCCGGACCGCTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                            <  2:3158682/140‑1 (MQ=255)

                                                                                                             aGCTGGTTGCGGGTGTGGATGATGTCGGGCGCGGGCCGTGAGTTGGCGTGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgcg                           <  2:2972532/140‑1 (MQ=255)

                                                                                                             aGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgcg                           <  1:1189094/140‑1 (MQ=255)

                                                                                                             aGCTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgcg                           <  2:6067564/140‑1 (MQ=255)

                                                                                                             aGCTGGTTGCGGGTGTGCATGAAGTCGGGCGCGGGCCGTGAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgcg                           <  2:2200381/140‑1 (MQ=255)

                                                                                                              gCTGGTTGCGGGTGTAGATGAAGTCAGGCACGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgcgc                          <  1:3219350/140‑1 (MQ=255)

                                                                                                               cTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGgcg                           <  1:4668734/138‑1 (MQ=255)

                                                                                                               cTGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGct                         <  2:1662295/140‑1 (MQ=255)

                                                                                                                tGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctc                        <  1:749533/140‑1 (MQ=255)

                                                                                                                tGGTTGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctc                        <  2:5482645/140‑1 (MQ=255)

                                                                                                                   ttGCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGATGACCGTAAAACGTCGTCTGGCGctcctg                     >  1:2777819/1‑137 (MQ=255)

                                                                                                                    tgctggTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGcc                                   >  2:4148571/5‑125 (MQ=255)

                                                                                                                    tGCTGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGcc                                   <  1:4148571/125‑1 (MQ=255)

                                                                                                                     gCGGGTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCCCCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGa                   <  1:5570341/140‑1 (MQ=255)

                                                                                                                       gggTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag                 <  1:5797169/140‑1 (MQ=255)

                                                                                                                       gggTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag                 <  2:6844292/140‑1 (MQ=255)

                                                                                                                       gggTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTCACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag                 <  1:1093049/140‑1 (MQ=255)

                                                                                                                       gggTGTGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGACCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag                 <  1:5119154/140‑1 (MQ=255)

                                                                                                                       gggTGTGGATGAAGTCGAGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag                 >  1:7095402/1‑140 (MQ=255)

                                                                                                                         gtgtGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAagag               >  2:6320518/1‑140 (MQ=255)

                                                                                                                         gtgtGGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAagag               >  2:7281941/1‑140 (MQ=255)

                                                                                                                           gtgGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGAt             <  1:5855542/140‑1 (MQ=255)

                                                                                                                            tgGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATc            >  1:915816/1‑140 (MQ=255)

                                                                                                                            tgGATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATc            >  1:131170/1‑140 (MQ=255)

                                                                                                                             ggATGAAGTCGGGCGCGGGCCGTTAGTTGGCGCGATCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCa           >  1:4875165/1‑140 (MQ=255)

                                                                                                                                   aGTCGGGCGCGTGCCGTTAGTTGTCGCGGTCGTCACCGCTTCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGTCGTCTGGCTCTCTCTGAAGAGATCAAAgaga     >  2:2429227/1‑140 (MQ=255)

                                                                                                                                   aGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAgaga     >  2:1135408/1‑140 (MQ=255)

                                                                                                                                   aGTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAgaga     >  1:1243542/1‑140 (MQ=255)

                                                                                                                                    gTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGaa    >  2:4760866/1‑140 (MQ=255)

                                                                                                                                    gTCGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGaa    >  2:710475/1‑140 (MQ=255)

                                                                                                                                      cGGGCGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAg  <  1:4535195/140‑1 (MQ=255)

CGATACCTTCCGTGAACTGCATCAGCAGATCCGCTGCAATGCCGATGAGCAGGCGGCACAAGCCGTTCTGGAGTTAGCACAATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATTGCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAG  >  NC_000913/204412‑204685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`