BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,190,871	G→A	D372D (GAC→GAT)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,190,871	0	G	A	99.7%	1050.1 / NA	295	D372D (GAC→GAT)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (133/161); minor base T (1/0); total (134/161)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

CTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAG  >  NC_000913/1190735‑1191005

cTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCa                                                                                                                                     <  1:895958/140‑1 (MQ=255)

cTTCGGCGTCAACGCCCTTACCTCGAGTCCGCTCTTTGAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCa                                                                                                                                     <  1:3606292/140‑1 (MQ=255)

   cGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTGCCAGTTGGGATGCAGTTCATCGAGCAGATGATCACgg                                                                                                                                  >  1:2781166/1‑140 (MQ=255)

   cGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACgg                                                                                                                                  <  2:1868695/140‑1 (MQ=255)

     gCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGtt                                                                                                                                <  1:6197082/140‑1 (MQ=255)

     gCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGtt                                                                                                                                <  2:5301918/140‑1 (MQ=255)

     gCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGtt                                                                                                                                <  1:4857577/140‑1 (MQ=255)

     gCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGtt                                                                                                                                <  1:23350/140‑1 (MQ=255)

     gCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGtt                                                                                                                                <  2:4979540/140‑1 (MQ=255)

     gCGTCAACGCGCTTACCCCGAGACAGCTCTTCCAGCTTCGCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCGGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGtt                                                                                                                                <  2:2205470/140‑1 (MQ=255)

      cGTCAACGCGCTTACCGCGAGTGAGGTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTc                                                                                                                               <  1:3062833/140‑1 (MQ=255)

          aaCGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACtt                                                                                                                           >  2:4572307/1‑140 (MQ=255)

           aCGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTc                                                                                                                          <  1:1445838/140‑1 (MQ=255)

               gcTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGt                                                                                                                      <  2:5605804/140‑1 (MQ=255)

               gcTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGt                                                                                                                      <  1:1339061/140‑1 (MQ=255)

                   aCCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGc                                                                                                                  >  1:7280248/1‑140 (MQ=255)

                   aCCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGc                                                                                                                  >  2:1503175/1‑140 (MQ=255)

                    ccGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCt                                                                                                                 <  1:4126476/140‑1 (MQ=255)

                      gcgAGTCAGCTCTTTGAGCTTCTCGAACGTTTTTTCGCTGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCa                                                                                                               <  1:3399486/140‑1 (MQ=255)

                        gagaCAGCTCTTTCAGTCTCTCCTACGGTTTTTAGCTGCAATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACg                                                                                                             <  2:3364844/140‑1 (MQ=255)

                        gAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACg                                                                                                             <  2:6240820/140‑1 (MQ=255)

                        gAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACg                                                                                                             <  1:1220131/140‑1 (MQ=255)

                        gAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACg                                                                                                             <  1:4646544/140‑1 (MQ=255)

                        gAGTCAGCTCTTTCAGCTTCTCATACGGTTTTTCGATTCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACg                                                                                                             <  1:3208500/140‑1 (MQ=255)

                         aGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGc                                                                                                            <  1:4831287/140‑1 (MQ=255)

                          gTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGcc                                                                                                           <  2:3795169/140‑1 (MQ=255)

                            cAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCtt                                                                                                         <  1:4057996/140‑1 (MQ=255)

                            cAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCtt                                                                                                         <  2:5771721/140‑1 (MQ=255)

                            cAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCtt                                                                                                         <  1:5300811/140‑1 (MQ=255)

                            cAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCtt                                                                                                         <  2:3724118/140‑1 (MQ=255)

                               ctctTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCa                                                                                                      <  2:449290/140‑1 (MQ=255)

                               ctctTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCa                                                                                                      <  2:6098257/140‑1 (MQ=255)

                                tctTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAg                                                                                                     <  1:1704983/140‑1 (MQ=255)

                                tctTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGCTTGCTCACGCCTTTCAg                                                                                                     <  1:1854299/140‑1 (MQ=255)

                                 ctTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAgg                                                                                                    <  2:4869500/140‑1 (MQ=255)

                                 ctTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAgg                                                                                                    <  2:5026844/140‑1 (MQ=255)

                                    tCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGc                                                                                                                  >  1:4233860/1‑123 (MQ=255)

                                    tCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTg                                                                                                 >  2:291179/1‑140 (MQ=255)

                                      aGCTTCTCTTACGGTTGTTCGAGGCCATAGCTACGTATACCTGTCTGGCTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGa                                                                                               <  2:3603717/140‑1 (MQ=255)

                                      aGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGa                                                                                               <  2:3846090/140‑1 (MQ=255)

                                         ttCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCtt                                                                                                         <  1:4400125/127‑1 (MQ=255)

                                            tcGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGa                                                                                               <  2:7275035/134‑1 (MQ=255)

                                            tcGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGata                                                                                         >  1:5008923/1‑140 (MQ=255)

                                            tcGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGata                                                                                         >  1:6026738/1‑140 (MQ=255)

                                            tcGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGata                                                                                         >  2:1734454/1‑140 (MQ=255)

                                              gTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATg                                                                                       <  2:4230643/140‑1 (MQ=255)

                                              gTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGAGCACGGTTCCCTTGCAGGTTGCTCACGCCTTTCAGGGTGGATTGATATg                                                                                       >  2:2993518/1‑140 (MQ=255)

                                               tACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGc                                                                                      <  2:4719346/140‑1 (MQ=255)

                                                   gTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATc                                                                                  <  2:4458198/140‑1 (MQ=255)

                                                   gTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATc                                                                                  <  2:6900914/140‑1 (MQ=255)

                                                         cGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCa                                                                            <  1:4447392/140‑1 (MQ=255)

                                                         cGATGCCATAGCGACGATTAACCGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCa                                                                            <  2:3283150/140‑1 (MQ=255)

                                                                   gacaggCATAACTGTCTGGATTCGTGCAGACAGCACTCCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATAc                                                                  <  1:2962613/135‑1 (MQ=255)

                                                                    cGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATAcc                                                                 <  1:7448295/140‑1 (MQ=255)

                                                                        gCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACg                                                             >  2:7177151/1‑140 (MQ=255)

                                                                        gCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACg                                                             >  2:592908/1‑140 (MQ=255)

                                                                        gCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAACCAAGGCATAACCGATACCCACg                                                             >  2:2823490/1‑140 (MQ=255)

                                                                                 tCTGGATTGGTTCAGCCTGCACCTCCCAGTTGTGAGCCATTTCATCCATCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTa                                                    <  2:2781166/140‑1 (MQ=255)

                                                                                 tCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTa                                                    >  1:3927259/1‑140 (MQ=255)

                                                                                 tCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTa                                                    >  2:4644834/1‑140 (MQ=255)

                                                                                   tGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACg                                                  <  1:706171/140‑1 (MQ=255)

                                                                                   tGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACg                                                  <  1:5402292/140‑1 (MQ=255)

                                                                                   tGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGCGGATTGATATGCAATCGAGGCATAAACGATACCCACGCCGAggctgtc                                                  >  2:2962613/1‑135 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCTTAATCGATACCCAAGCCGAGGTTACgctg                                               >  1:3002036/1‑138 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACg                                                             <  2:4588610/126‑1 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACg                                                             >  1:4588610/1‑126 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAgg                                                       <  2:6983478/132‑1 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACgcag                                               <  1:647674/140‑1 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACgcag                                               <  1:7177151/140‑1 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACgcag                                               <  1:6670141/140‑1 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACgcag                                               <  1:5644965/140‑1 (MQ=255)

                                                                                      aTTGGTTCAGCCAGCACCTCCCTGTTGTGATCCAGTTCACCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGAAACCCACGCCGAGGTTACgcag                                               <  2:2247990/140‑1 (MQ=255)

                                                                                         ggTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCAc                                            <  1:4773206/140‑1 (MQ=255)

                                                                                          gTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACg                                           >  1:1030295/1‑140 (MQ=255)

                                                                                               gccagcACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGGAATCAAGGCATAACCGATACCCACGACGAGCTTACGCAGCACGGTaga                                      >  2:3146698/1‑140 (MQ=255)

                                                                                                ccagcACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATGACCGATAGCCAGGCGGAGGTTGCGCAGCACGGTagag                                     >  1:2201091/1‑140 (MQ=255)

                                                                                                          ccAGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCggtcaggt                           >  2:6818837/1‑140 (MQ=255)

                                                                                                            aGTTGTGATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCAGGCCGAGGTTACGCAGCACGGTCGAGTCggtcaggtca                         >  1:2902037/1‑140 (MQ=255)

                                                                                                                 tgATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTg                    >  2:5123734/1‑140 (MQ=255)

                                                                                                                 tgATCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTg                    >  2:7434584/1‑140 (MQ=255)

                                                                                                                   aTCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACTGTAGAGTCGCTCAGGTCACGCTGcc                  >  1:2413922/1‑140 (MQ=255)

                                                                                                                   aTCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                  >  2:6503194/1‑140 (MQ=255)

                                                                                                                   aTCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                  >  1:104912/1‑140 (MQ=255)

                                                                                                                   aTCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                  >  1:735937/1‑140 (MQ=255)

                                                                                                                   aTCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                  >  1:6776041/1‑140 (MQ=255)

                                                                                                                   aTCCAGTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                  >  1:5188925/1‑140 (MQ=255)

                                                                                                                        ggtCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCTGTCAGGTCACGCTGCCAGCgg             >  1:5121763/3‑140 (MQ=255)

                                                                                                                        gTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACTCAGCACGGTAGAGTGGGTCAGATCACGCTGCCAGCgg             >  1:2527681/1‑140 (MQ=255)

                                                                                                                        gTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCTAGCgg             >  1:75939/1‑140 (MQ=255)

                                                                                                                        gTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg             >  1:1815464/1‑140 (MQ=255)

                                                                                                                        gTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg             >  1:4705025/1‑140 (MQ=255)

                                                                                                                        gTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg             >  1:6825279/1‑140 (MQ=255)

                                                                                                                        gTTCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg             >  2:6698469/1‑140 (MQ=255)

                                                                                                                         ttCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCggg            >  2:826001/1‑140 (MQ=255)

                                                                                                                         ttCATCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCggg            >  1:6965086/1‑140 (MQ=255)

                                                                                                                             tCCAGCAGATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAAc        >  2:4271155/1‑140 (MQ=255)

                                                                                                                                 gcagATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTTCGCAGCACGGAATAGTCAGTCAGGTAACGCGTCCAGCGGGAAACCAGc    >  2:2509785/1‑140 (MQ=255)

                                                                                                                                  cagATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCa   >  1:4173840/1‑140 (MQ=255)

                                                                                                                                  cagATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCa   <  2:4348794/140‑1 (MQ=255)

                                                                                                                                   agATGATCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAg  >  2:4401589/1‑140 (MQ=255)

CTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAG  >  NC_000913/1190735‑1191005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`