Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,432,780	T→C	F124L (TTC→CTC)	tfaR →	Rac prophage; putative tail fiber assembly protein TfaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,432,780	0	T	C	100.0%	44.7 / NA	21	F124L (TTC→CTC)	tfaR	Rac prophage; putative tail fiber assembly protein TfaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/5);  total (16/5)

CGTGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGTTCCGGATCCGGGAGGCGGAAGAAACAAAAAACAACCTGATGCAGGTAGCCAGTGAGCATATTGCGCCGCTTCAGGATGCTGCAGATCTGGAAATTGCAACGGAGGAAGAAATCTCGT  >  NC_000913/1432644‑1432897                                                                                                                                         |                                                                                                                      cGTGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTcc                                                                                                                    <  2:6219124/140‑1 (MQ=17)  gTGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGCTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCg                                                                                                                   <  1:2049017/140‑1 (MQ=12)  gTGGCTTCCGGCAACGCCTTATTTATTTCTGCACTAGGTTCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCg                                                                                                                   <  2:2967409/140‑1 (MQ=11)   tGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACCGCCTGGGAGAAGGATAAGGAAGCAGAAAAACTGCTCCgg                                                                                                                  >  2:2621365/1‑140 (MQ=255)   tGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCgg                                                                                                                  >  1:4228707/1‑140 (MQ=18)   tGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCgg                                                                                                                  >  1:4412339/1‑140 (MQ=18)   tGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCgg                                                                                                                  >  1:5492989/1‑140 (MQ=18)   tGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCgg                                                                                                                  >  2:1612843/1‑140 (MQ=18)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACATCCTGGGTAAAGGATACGGAAGGAGAAAAACTGCTCCGGa                                                                                                                 >  2:2279763/1‑140 (MQ=255)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTc                                                                                                                     >  1:6232159/1‑136 (MQ=18)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTc                                                                                                                     >  1:7261299/1‑136 (MQ=17)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGa                                                                                                                 >  1:1437579/1‑140 (MQ=17)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGa                                                                                                                 >  1:5868949/1‑140 (MQ=17)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGa                                                                                                                 >  2:5491775/1‑140 (MQ=18)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGCTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGa                                                                                                                 >  2:6660477/1‑140 (MQ=17)    ggCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGACCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCATGGGTGAGGGAAACAGAAGCAGAAAAACTGCTCCGGa                                                                                                                 >  1:1971648/1‑140 (MQ=11)      cTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGATc                                                                                                               >  2:6893198/1‑140 (MQ=18)        tCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGATCCg                                                                                                             <  2:5357845/140‑1 (MQ=17)          cGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGATCCggg                                                                                                           >  2:5862327/1‑140 (MQ=18)          cGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGATGACGGAGACGGAAGCGGAAATACTGCTCCGGATCCggg                                                                                                           >  2:2340823/1‑140 (MQ=11)           ggCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGATCCGGGa                                                                                                          >  1:4384707/1‑140 (MQ=18)            gCAACGCGTTATTTATTTCTGAACTCGGTCCTTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGCTCCGGATCCGGGAg                                                                                                         <  2:2789212/140‑1 (MQ=12)                                                                                                                   ggctACGGAAGCAGAGAAACTGCTCCGGATCCGGGAGGCGGAAGAAACAAAAAACAACCTGATGCAGGTAGCCAGTGAGCATATTGCGCCGCTTCAGGATGCTGCAGATCTGGCAATTGCAACGGAGGAAGAAATCTCgc  >  2:3837163/4‑139 (MQ=0)                                                                                                                                         |                                                                                                                      CGTGGCTTCCGGCAACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAATGTTACCTGGTTATCGCCGGAAGGGGAGTTTCAGAAGTGGAACGGCACAGCCTGGGTGAAGGATACGGAAGCAGAAAAACTGTTCCGGATCCGGGAGGCGGAAGAAACAAAAAACAACCTGATGCAGGTAGCCAGTGAGCATATTGCGCCGCTTCAGGATGCTGCAGATCTGGAAATTGCAACGGAGGAAGAAATCTCGT  >  NC_000913/1432644‑1432897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑