Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,444,488 A→G M480V (ATG→GTG)  ydbH → PF11739 family protein YdbH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,444,4880AG99.6% 887.8 / NA 240M480V (ATG→GTG) ydbHPF11739 family protein YdbH
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (169/70);  total (170/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGC  >  NC_000913/1444352‑1444623
                                                                                                                                        |                                                                                                                                       
gTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGa                                                                                                                                      >  1:5818740/1‑140 (MQ=255)
 tGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGAc                                                                                                                                     <  1:6701414/140‑1 (MQ=255)
             ggAAAAGGGAGTTTTCCACCGATGAGTGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGcc                                                                                                                         <  1:2702066/140‑1 (MQ=255)
             ggAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGcc                                                                                                                         <  2:3769511/140‑1 (MQ=255)
                 aaGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGGGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCCGTTACAAGACGATAAGGGGACGGTAGAAAAGCCGCGa                                                                                                                     >  1:2377814/1‑140 (MQ=255)
                   gggAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGAtt                                                                                                                   >  1:190459/1‑140 (MQ=255)
                      aGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAAt                                                                                                                >  1:4467428/1‑140 (MQ=255)
                      aGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAAt                                                                                                                >  2:650299/1‑140 (MQ=255)
                        ttttACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATtc                                                                                                              <  1:5624896/140‑1 (MQ=255)
                               ccGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCACCCGGTTTCGACCAGTTACAATACGTTACGGTGACGGTAGAATAGCCACGATTAATTCTCGCCaa                                                                                                       >  2:2314494/1‑140 (MQ=255)
                                cGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACTGTGACGGTAGAAAAGCCGCGATTAATTCTCGACaaa                                                                                                      >  2:6988230/1‑139 (MQ=255)
                                cGATGAATGCCACCTGGAATGTAGCAGGAAATGGTAAGTTGCATGACACCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAg                                                                                                      <  2:2377814/140‑1 (MQ=255)
                                  aTGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACGATACGGTACGGTGACGATAGAAAAGACGCGATTAATTCTCGACAAGcc                                                                                                    >  2:3039523/1‑140 (MQ=255)
                                  aTGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGa                                                                                                          <  2:6348299/134‑1 (MQ=255)
                                  aTGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGa                                                                                                          >  1:6348299/1‑134 (MQ=255)
                                  aTGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGcc                                                                                                    >  2:5180331/1‑140 (MQ=255)
                                  aTGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGcc                                                                                                    >  1:5029401/1‑140 (MQ=255)
                                  aTGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATACTCGACAAGcc                                                                                                    >  1:5932560/1‑140 (MQ=255)
                                    gAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCa                                                                                                  <  1:5968810/140‑1 (MQ=255)
                                    gAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCa                                                                                                  <  2:122709/140‑1 (MQ=255)
                                    agacagcACCTGGGATGCCGCAAGAAAAGGTCAGTCGCACGACAGCACCATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCa                                                                                                  <  2:1982650/134‑1 (MQ=255)
                                       tGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCa                                                                                                  >  2:4349819/1‑137 (MQ=255)
                                         ccACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTc                                                                                             <  2:1128224/140‑1 (MQ=255)
                                          cACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCt                                                                                            <  2:7517227/140‑1 (MQ=255)
                                                 gATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTTATTTTCTACAAGCGCATCGTCTGGGTACg                                                                                     >  1:2529167/1‑140 (MQ=255)
                                                     tCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGGCAAGCCCATAGTCTGGGTACGTGAc                                                                                 >  1:2470704/1‑140 (MQ=255)
                                                     tCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGAc                                                                                 >  1:893974/1‑140 (MQ=255)
                                                     tCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGAc                                                                                 >  1:1442180/1‑140 (MQ=255)
                                                     tCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCAATTAATTGTCGCCATGCCCATGGTCTGGGTActgtct                                                                                 >  1:1982650/1‑135 (MQ=255)
                                                      cGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACg                                                                                >  1:1025629/1‑140 (MQ=255)
                                                      cGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACg                                                                                >  1:7309308/1‑140 (MQ=255)
                                                      cGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATGAATACTCGACAACCCCACCGTCTGGGTACGTGACg                                                                                >  2:2880559/1‑140 (MQ=255)
                                                       gCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGc                                                                               >  1:7580427/1‑140 (MQ=255)
                                                            aaaaGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGc                                                                          <  1:650299/140‑1 (MQ=255)
                                                                ggTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATcc                                                                      >  2:1588945/1‑140 (MQ=255)
                                                                    aGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCcctcc                                                                  >  1:6047036/1‑140 (MQ=255)
                                                                     gTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCcctcct                                                                 >  1:5261337/1‑140 (MQ=255)
                                                                     gTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCcctcct                                                                 >  2:550447/1‑140 (MQ=255)
                                                                      tGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCtt                                                                >  1:3827280/1‑140 (MQ=255)
                                                                              cAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAgcggcg                                                        <  2:6234173/140‑1 (MQ=255)
                                                                                gCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGgcgcg                                                      <  1:4182709/140‑1 (MQ=255)
                                                                                gCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGgcgcg                                                      <  1:3930460/140‑1 (MQ=255)
                                                                                gCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGgcgcg                                                      >  2:5655144/1‑140 (MQ=255)
                                                                                 cACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGgcgcgc                                                     >  2:840063/1‑140 (MQ=255)
                                                                                              aCCGATCTCTCCACCGGTTTCGACCGGTTTCAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGc                                        <  1:2809994/140‑1 (MQ=255)
                                                                                              aCCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGc                                        >  2:4720818/1‑140 (MQ=255)
                                                                                              aCCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGAACAGCATGCCTCCTTTGGCGGCGCGCTGTCACTGGACGc                                        >  2:2738488/1‑140 (MQ=255)
                                                                                              aCCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGCACGTGACGAACAGCGTACCTCCTTTCGCGGCGCGCTGTCCCTGGACGc                                        >  2:3505597/1‑140 (MQ=255)
                                                                                                    ctctcCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCa                                  >  1:1289818/1‑140 (MQ=255)
                                                                                                      ctcCACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCaaa                                <  1:4720818/140‑1 (MQ=255)
                                                                                                        ccACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACg                              >  1:1086155/1‑140 (MQ=255)
                                                                                                        ccACCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACg                              >  1:4414292/1‑140 (MQ=255)
                                                                                                         cACCGGTTTCGACCAGTTACAATACGGTACGGTAACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGc                             <  2:1341868/140‑1 (MQ=255)
                                                                                                          aCCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGC‑‑CGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGc                                        <  2:16698/126‑1 (MQ=255)
                                                                                                          aCCGGTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGC‑‑CGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGc                                        >  1:16698/1‑126 (MQ=255)
                                                                                                             ggTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGtt                         >  2:6692655/1‑140 (MQ=255)
                                                                                                              gTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTc                        >  1:1213683/1‑140 (MQ=255)
                                                                                                              gTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTc                        >  2:6902381/1‑140 (MQ=255)
                                                                                                              gTTTCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTc                        >  1:7083837/1‑140 (MQ=255)
                                                                                                               tttCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCa                       >  2:3913081/1‑140 (MQ=255)
                                                                                                                 tCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACt                     >  2:3840541/1‑140 (MQ=255)
                                                                                                                 tCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACt                     >  2:4199231/1‑140 (MQ=255)
                                                                                                                 tCGACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACt                     >  2:1390805/1‑140 (MQ=255)
                                                                                                                   gACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTgg                   >  2:5051210/1‑140 (MQ=255)
                                                                                                                   gACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTgg                   >  2:4930824/1‑140 (MQ=255)
                                                                                                                   gACCAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTAGACGCCGGGCAAACGCTGTTCACTgg                   <  2:5261337/140‑1 (MQ=255)
                                                                                                                      cAGTTACAATACGGTCCGGTGACGGCAGACAAGCCGCAATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTggcgg                <  1:2148263/140‑1 (MQ=255)
                                                                                                                      cAGTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTggcgg                <  2:5818740/140‑1 (MQ=255)
                                                                                                                        gTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCa              <  1:5180331/140‑1 (MQ=255)
                                                                                                                        gTTACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCa              <  2:3792116/140‑1 (MQ=255)
                                                                                                                         ttACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTgg                   >  1:4552583/1‑134 (MQ=255)
                                                                                                                         ttACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTgg                   <  2:4552583/134‑1 (MQ=255)
                                                                                                                         ttACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAg             >  2:5716565/1‑140 (MQ=255)
                                                                                                                         ttACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAg             >  1:1805445/1‑140 (MQ=255)
                                                                                                                         ttACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAg             >  2:6684799/1‑140 (MQ=255)
                                                                                                                         ttACAATACGGTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAg             >  2:4460834/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTATGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  2:4806209/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGAGGCCGGGCAAACGCTGTACACTAGCGGCAGTGTGTTACCg   >  2:1916814/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGGCGGGCAAACGCTGTTCACTGGTGGCAGTGTGTTACCg   >  1:3286994/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAGCGCAGTACACTGGCGGCAGTGTGTTACCg   >  1:3540419/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTAccc   >  2:6121279/1‑139 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTAc     <  2:5951534/138‑1 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  1:6862209/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  2:7125938/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  2:7339995/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  2:1460095/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   <  2:5518368/140‑1 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  2:5177790/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCg   >  1:6770558/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCCGACAAACGCTGTCCACTCACGGCAGTGTGTTACCg   >  2:3335415/1‑140 (MQ=255)
                                                                                                                                   gTACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGAAGCCAGGCGAACGCTGTACACTGGCGGCAGTGTGTTAccc   >  2:2524797/1‑139 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  1:3756182/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  1:3985830/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  2:5405404/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  1:4279586/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  1:7223405/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  2:6894528/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  2:4105515/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  2:7291199/1‑140 (MQ=255)
                                                                                                                                    tACGGTGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGc  >  1:548746/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGC  >  NC_000913/1444352‑1444623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑