BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	209,910	C→T	P78S (CCG→TCG)	ldcC →	lysine decarboxylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	209,910	0	C	T	99.2%	1654.1 / NA	479	P78S (CCG→TCG)	ldcC	lysine decarboxylase 2
Reads supporting (aligned to +/- strand): ref base C (0/4); new base T (253/221); total (253/226)
Fisher's exact test for biased strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 493 total aligned reads displayed.

TATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCCCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGA  >  NC_000913/209777‑210046

tATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGGAGCGATAGCAAGCAACTTAATAAATATCTCTCGCttt                                                                                                                                    >  1:2417684/1‑140 (MQ=255)

 aTCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTa                                                                                                                                   >  1:672669/1‑140 (MQ=255)

   cTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATg                                                                                                                                 <  1:5758952/140‑1 (MQ=255)

     ggCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGcc                                                                                                                               <  2:837588/140‑1 (MQ=255)

      gCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCt                                                                                                                              >  1:7277601/1‑140 (MQ=255)

      gCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCt                                                                                                                              <  1:6857832/140‑1 (MQ=255)

      gCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCt                                                                                                                              <  1:4444053/140‑1 (MQ=255)

      gCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGAGATCAATCGGCTCAAGGAATATATCTCGCTTTATGCCt                                                                                                                              >  1:2011928/1‑140 (MQ=255)

       ccACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCtt                                                                                                                             <  2:6692846/140‑1 (MQ=255)

        cacaAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGcc                                                                                                                               <  1:6934057/137‑1 (MQ=255)

        cacaAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTtc                                                                                                                            >  1:659358/1‑140 (MQ=255)

           aaaaCAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTtcat                                                                                                                          <  2:3945225/139‑1 (MQ=255)

           aaaaCAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTtcat                                                                                                                          >  1:3945225/1‑139 (MQ=255)

                aGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCt                                                                                                                              >  1:1183902/1‑130 (MQ=255)

                aGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAacac                                                                                                                    <  1:5422956/140‑1 (MQ=255)

                 gCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTGGCTTTATGCCTTCATCAACAcc                                                                                                                   >  2:3178120/1‑140 (MQ=255)

                  cGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATGTCAATCAGATTAGTGATTATCTCTCTCTTTGTGCCTTCATCAACAccc                                                                                                                  >  2:2010204/1‑140 (MQ=255)

                  cGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACAccc                                                                                                                  >  2:6313016/1‑140 (MQ=255)

                  cGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACAccc                                                                                                                  >  1:7517646/1‑140 (MQ=255)

                      gATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTc                                                                                                              <  2:5861807/140‑1 (MQ=255)

                          tGCTGAAATTTATCGCGCATAATCCCCGATTTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTcgacg                                                                                                          <  1:2321393/140‑1 (MQ=255)

                          tGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTcgacg                                                                                                          <  1:3438366/140‑1 (MQ=255)

                          tGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTcgacg                                                                                                          <  1:4052421/140‑1 (MQ=255)

                          tGCTGAAATTTATCGAGAATAACCCTCGAATTTGCAGCGAGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTcgacg                                                                                                          <  2:2175181/140‑1 (MQ=255)

                            cTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCCTAATGAATGTCTATCGATTTATGCCGTCATCAACACCCACTCGACgat                                                                                                        >  2:2321393/1‑140 (MQ=255)

                               aaaTTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACgatgga                                                                                                     <  1:4872262/140‑1 (MQ=255)

                                    tATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTCGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCa                                                                                                <  1:4656301/140‑1 (MQ=255)

                                    tATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCa                                                                                                <  2:5439124/140‑1 (MQ=255)

                                    tATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCa                                                                                                <  2:7445000/140‑1 (MQ=255)

                                    tATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCa                                                                                                <  1:1735761/140‑1 (MQ=255)

                                    tATCGAGCATAACCCTCCAATTGGCGGCGTGGTTTATGACTGGGATGACTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCa                                                                                                <  2:2902654/140‑1 (MQ=255)

                                      tCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGc                                                                                              >  2:4334176/1‑140 (MQ=255)

                                      tCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGc                                                                                              >  2:4887860/1‑140 (MQ=255)

                                        gAGCATAACCCTCGACTTTGCGTCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGt                                                                                            <  1:3697931/140‑1 (MQ=255)

                                          gCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGc                                                                                          <  2:1779311/140‑1 (MQ=255)

                                          gCATAACCCTCGAATCTGCGGCATGATTTGTGACTGGCATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGc                                                                                          <  1:2552554/140‑1 (MQ=255)

                                                   tCGAATTTGCGGCGTGATTTTTCACGGGGATGAGTACCGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGc                                                                                 <  1:3258910/140‑1 (MQ=255)

                                                       aTTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGAt                                                                             <  2:524086/140‑1 (MQ=255)

                                                        tttGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATg                                                                            <  2:696546/140‑1 (MQ=255)

                                                          tGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGgc                                                                          <  1:5671473/140‑1 (MQ=255)

                                                          tGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGgc                                                                          >  1:6013884/1‑140 (MQ=255)

                                                           gcggcgTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAAAATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGgcg                                                                         >  1:652281/1‑140 (MQ=255)

                                                             ggcgTGATTTTTGCCTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGct                                                                       <  2:7497168/140‑1 (MQ=255)

                                                             ggcgTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGct                                                                       <  2:5771629/140‑1 (MQ=255)

                                                                         gACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGa                                                           >  1:5310964/1‑140 (MQ=255)

                                                                           cTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAat                                                         <  2:1544969/140‑1 (MQ=255)

                                                                           cTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAat                                                         <  2:5941123/140‑1 (MQ=255)

                                                                           cTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAat                                                         <  1:5891047/140‑1 (MQ=255)

                                                                              ggATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAat                                                         <  2:6552572/137‑1 (MQ=255)

                                                                              ggATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATg                                                      >  1:5039922/1‑140 (MQ=255)

                                                                              ggATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGAATAGCGCTCTGGTTTTTTGAATATg                                                      >  1:2773524/1‑140 (MQ=255)

                                                                                     tACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCC‑TCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTgggg                                              <  2:5655412/140‑1 (MQ=255)

                                                                                      aCAGTCTCGATTTATGTAGCGAGATCAATCACCTTTATGCATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTgggg                                              <  2:2081300/140‑1 (MQ=255)

                                                                                       cAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGc                                             <  1:6821791/140‑1 (MQ=255)

                                                                                         gTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCAGATGGCGCTCTTGTCTTTTGAATATGCGCTGGGGCAg                                           >  1:2659118/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAat                                                         <  2:1121142/125‑1 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAgg                                          >  2:5584088/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAgg                                          >  1:1401470/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAgg                                          >  1:7371865/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAgg                                          >  1:4779354/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAgg                                          <  2:7277601/140‑1 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAgg                                          >  1:4001327/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTGTTTGAATATGCGCTGGGGCAgg                                          >  1:2167800/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGATTTTTTGAATATGCGCTGGGGCAgg                                          >  1:2930995/1‑140 (MQ=255)

                                                                                          tctcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGAGGATGGCGGTCTGGTTATTTGAGTATGCGCGGGGGCAgg                                          >  2:3582447/1‑140 (MQ=255)

                                                                                            tcGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCg                                        <  2:6085756/140‑1 (MQ=255)

                                                                                             cGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCgg                                       >  2:4550645/1‑140 (MQ=255)

                                                                                               aTTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGaa                                     >  1:981932/1‑140 (MQ=255)

                                                                                               aTTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGaa                                     >  2:4674848/1‑140 (MQ=255)

                                                                                               aTTTATGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGAGCTCTGGGTTTCTGAATATGCGCTGGGGCAGGCGGaa                                     >  1:3023822/1‑140 (MQ=255)

                                                                                                    tGTAGCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGatat                                >  2:1239063/1‑140 (MQ=255)

                                                                                                        gCGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCC‑TCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCa                           <  2:4035478/140‑1 (MQ=255)

                                                                                                         cGATATCAATCAGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCa                           <  2:2541613/140‑1 (MQ=255)

                                                                                                         cGATATCAATCAGCTTAATGAACATCTCTCGCTTTTTGCATTCATCAATACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCa                           <  2:2659118/140‑1 (MQ=255)

                                                                                                              tcaatcaGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGt                      >  2:1635402/1‑140 (MQ=255)

                                                                                                              tcaatcaGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGt                      >  2:684012/1‑140 (MQ=255)

                                                                                                               caatcaGCTTAATGACTATCTCTCGATTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTa                     <  1:3178120/140‑1 (MQ=255)

                                                                                                               caatcaGCTTAATGACTATCTCCCGGTTTCTGCCTTCCTCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTa                     <  1:1931693/140‑1 (MQ=255)

                                                                                                               caatcaGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTa                     <  2:4199676/140‑1 (MQ=255)

                                                                                                                 atcaGCTTAATGAATGTCTCTCTCTGTATGCCTTCATTAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATg                   <  1:2510768/140‑1 (MQ=255)

                                                                                                                 atcaGCTTAATGAATATGTCTCCCTTTATGCTTTCTTCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATg                   <  2:3023822/140‑1 (MQ=255)

                                                                                                                 atcaGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATg                   <  2:4779354/140‑1 (MQ=255)

                                                                                                                 atcaGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATg                   <  2:1262332/140‑1 (MQ=255)

                                                                                                                 atcaGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATg                   <  1:631209/140‑1 (MQ=255)

                                                                                                                   caGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCg                 >  2:7100962/1‑140 (MQ=255)

                                                                                                                    aGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCATCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGt                <  1:1028662/140‑1 (MQ=255)

                                                                                                                    aGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGt                <  1:215666/140‑1 (MQ=255)

                                                                                                                    aGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGt                <  1:1615125/140‑1 (MQ=255)

                                                                                                                    aGCTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTCTTTGAATACGCGCTTGGGCAGGCGGAAGATATCGCCATTCGTATGCGt                >  2:3254187/1‑140 (MQ=255)

                                                                                                                      cTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCa              >  1:6124194/1‑140 (MQ=255)

                                                                                                                      cTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCa              >  2:677482/1‑140 (MQ=255)

                                                                                                                      cTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCa              >  2:5017812/1‑140 (MQ=255)

                                                                                                                      cTTAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCa              >  2:382393/1‑140 (MQ=255)

                                                                                                                        tAATGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCACTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGt            <  1:4269584/140‑1 (MQ=255)

                                                                                                                         aaTGAATATCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTa                     >  1:842742/1‑130 (MQ=255)

                                                                                                                           tGAATATCTCTCGCTTTATGCCATCATCAACCCCCACTCTACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTaca         <  2:2485578/140‑1 (MQ=255)

                                                                                                                                atCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACcgac    >  1:6402944/1‑140 (MQ=255)

                                                                                                                                atCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACcgac    >  1:7097157/1‑140 (MQ=255)

                                                                                                                                atCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACcgac    >  2:1845879/1‑140 (MQ=255)

                                                                                                                                atCTCTCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACcgac    >  1:5588990/1‑140 (MQ=255)

TATCTGGCCACAAAACAGCGTTGATTTGCTGAAATTTATCGAGCATAACCCTCGAATTTGCGGCGTGATTTTTGACTGGGATGAGTACAGTCTCGATTTATGTAGCGATATCAATCAGCTTAATGAATATCTCCCGCTTTATGCCTTCATCAACACCCACTCGACGATGGATGTCAGCGTGCAGGATATGCGGATGGCGCTCTGGTTTTTTGAATATGCGCTGGGGCAGGCGGAAGATATCGCCATTCGTATGCGTCAGTACACCGACGA  >  NC_000913/209777‑210046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`