Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,229,182	(G)5→4	intergenic (+117/+256)	yohP → / ← dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,229,178	0	G	.	100.0%	124.2 / NA	36	intergenic (+113/+260)	yohP/dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/14);  total (22/14)

GCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTC  >  NC_000913/2229043‑2229312                                                                                                                                        |                                                                                                                                       gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggc                                                                                                                                    >  2:606414/1‑139 (MQ=255) gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggc                                                                                                                                    >  1:44519/1‑139 (MQ=255)            atgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGTGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAAGACCGCGCA‑GGGGCGGCCAATcgcc                                                                                                                        >  1:228837/1‑140 (MQ=255)                   tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGccc                                                                                                                 >  1:123671/1‑140 (MQ=255)                         attaattaATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCTCTGGCGCAAGGATCTCGCA‑GGGGCGTCCAATCGCCGCCGCCCCCTGCa                                                                                                           >  2:287367/1‑140 (MQ=38)                         attaattaATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCa                                                                                                           <  1:580339/140‑1 (MQ=255)                            aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCAccc                                                                                                        >  1:103638/1‑140 (MQ=255)                                      atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTg                                                                                              >  2:565537/1‑140 (MQ=255)                                      atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTg                                                                                              >  2:26386/1‑140 (MQ=255)                                           gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGTCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGaa                                                                                         >  2:254458/1‑140 (MQ=255)                                                     aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                               >  1:62911/1‑140 (MQ=255)                                                     aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                               >  1:404411/1‑140 (MQ=255)                                                             tatTTCAGTCTCTCATTCTGTTCCGCTCCTAACATTTGCTTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                       <  2:322202/140‑1 (MQ=18)                                                             tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                       >  2:443258/1‑140 (MQ=255)                                                             tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                       <  1:521135/140‑1 (MQ=255)                                                              atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCgg                                                                      <  2:139641/140‑1 (MQ=255)                                                              atTTCAGTCTCTCATCCGGTTGAGCTCCTGTGATGTGCACTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCgg                                                                      <  2:187168/140‑1 (MQ=18)                                                               tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                     >  2:373753/1‑140 (MQ=255)                                                               tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCAACCCCGGGATCTAGCAAACAAAGTCGCCGCTGCGCggc                                                                     >  1:282053/1‑139 (MQ=21)                                                                    gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCACCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                <  1:373382/140‑1 (MQ=255)                                                                     tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                               >  2:87774/1‑140 (MQ=255)                                                                           aTCCTGTTGTGCTCCTGCCATGCGCATTGCTCCATATAATCACTGGCGTAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                         <  2:228837/140‑1 (MQ=17)                                                                                 ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCtt                                                   >  2:607489/1‑140 (MQ=255)                                                                                                  gCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                  <  1:607489/140‑1 (MQ=255)                                                                                                   cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgc                                 <  2:103638/140‑1 (MQ=255)                                                                                                   cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgc                                 >  1:373663/1‑140 (MQ=255)                                                                                                   cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGAGGTGCCCACGGCTTATTCCTTCCGGCTACCGGGTCGGgc                                 >  2:192095/1‑140 (MQ=14)                                                                                                     ttGCTTCATATAATCTCTGGCGTAAAGAGCGCGCA‑GGCGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                               <  1:192095/140‑1 (MQ=14)                                                                                                        cTTCATATAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                            >  1:434327/1‑140 (MQ=255)                                                                                                            atatAATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATACGGCTACCGGGTCGGGCGCGAGGTaa                        <  1:502063/140‑1 (MQ=255)                                                                                                             tataATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCCCGAGGGAAc                       >  1:389548/1‑140 (MQ=255)                                                                                                             tataATCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                      <  1:26386/140‑1 (MQ=255)                                                                                                                  tCACTGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGGAACATccc                  >  1:542092/1‑140 (MQ=16)                                                                                                                      tGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                <  1:433525/122‑1 (MQ=17)                                                                                                                      tGGCGCAAGGAGCGCGCA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                >  2:433525/1‑122 (MQ=17)                                                                                                                           cAAGGAGCGCGCA‑GGGGCGGCCCATAGCCTCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg         <  1:287367/140‑1 (MQ=11)                                                                                                                                  cgcgcA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc  >  2:520838/1‑140 (MQ=17)                                                                                                                                  cgcgcA‑GGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCCGGTCGGGCGCGAGGTAACATCCCCGTAAAACCCGccccc  >  2:610569/1‑138 (MQ=11)                                                                                                                                        |                                                                                                                                       GCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTC  >  NC_000913/2229043‑2229312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑