Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,427	(C)5→4	intergenic (+68/‑91)	proY → / → malZ	putative transporter ProY/maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,423	0	C	.	100.0%	194.9 / NA	46	intergenic (+64/‑95)	proY/malZ	putative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/26);  total (20/26)

CGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAA  >  NC_000913/422286‑422558                                                                                                                                          |                                                                                                                                        cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGTCGGTTCAGGAGGTAATGCTGATCCGGCTATTCCGAAAGTTATCCGccc                                                                                                                                       >  2:267053/1‑140 (MQ=255)        tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGtcctc                                                                                                                               <  2:615631/140‑1 (MQ=255)        tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGtcctc                                                                                                                               <  1:37366/140‑1 (MQ=255)               cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCTGCTATTCGGAAGGTGATCCG‑CGCCGTCCTCCTccccc                                                                                                                        >  1:172965/1‑140 (MQ=255)               cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTccccc                                                                                                                        >  2:81923/1‑140 (MQ=255)               cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTccccc                                                                                                                        >  2:426909/1‑140 (MQ=255)                tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCa                                                                                                                       <  1:609873/140‑1 (MQ=255)                tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCAAAACTTGTCCG‑CCTCGTCCTCCTCCCCCa                                                                                                                       >  1:306005/1‑140 (MQ=255)                  ttGATTGGCTGGATGTTTAAACGCGGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCaaa                                                                                                                     >  2:445271/1‑140 (MQ=255)                  ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGtcctc                                                                                                                               >  2:97149/1‑130 (MQ=255)                  ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGtcctc                                                                                                                               <  1:97149/130‑1 (MQ=255)                  ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCaaa                                                                                                                     >  1:155668/1‑140 (MQ=255)                     aTTGGCTGGATGTTTACCCGCCTCCACGATCATCATCTGCGTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAatat                                                                                                                  <  2:286923/140‑1 (MQ=255)                         gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                              <  1:445271/140‑1 (MQ=255)                          cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                             >  2:438261/1‑140 (MQ=255)                          cTGGATGTTTAAACGCCCCCACTATCGTCAGCTGGTTGAGAACCAGTATTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATACG‑CCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                             <  2:198834/140‑1 (MQ=255)                               tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                        <  1:438261/140‑1 (MQ=255)                               tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                        <  2:587261/140‑1 (MQ=255)                                   tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                    >  2:496402/1‑140 (MQ=255)                                   tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                    >  1:535343/1‑140 (MQ=255)                                    aaaCGCCGCCACGATTGTCAGCTGGCTGAGAACCAGTCATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                   <  1:267053/140‑1 (MQ=255)                                    aaaCGCCGCCACGATGGTCAGCGGGTTGACAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                   <  1:306195/140‑1 (MQ=255)                                    aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCACTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                   <  1:557257/140‑1 (MQ=255)                                       cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                >  1:364548/1‑140 (MQ=255)                                         ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                              <  2:331644/140‑1 (MQ=255)                                         ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                              <  1:332282/140‑1 (MQ=255)                                         ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                              <  1:426909/140‑1 (MQ=255)                                               cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTGTTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                        <  1:560499/140‑1 (MQ=255)                                               cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                        <  1:372858/140‑1 (MQ=255)                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                           >  1:378788/1‑140 (MQ=255)                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                           >  1:362708/1‑140 (MQ=255)                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                           >  2:27920/1‑140 (MQ=255)                                                                            ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                           >  2:387566/1‑140 (MQ=255)                                                                             cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                          <  2:155668/140‑1 (MQ=255)                                                                             cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                          <  1:443962/140‑1 (MQ=255)                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                        <  2:378788/140‑1 (MQ=255)                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                        <  1:81923/140‑1 (MQ=255)                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                        <  2:535343/140‑1 (MQ=255)                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                >  2:632213/1‑140 (MQ=255)                                                                                             cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                          >  2:348858/1‑140 (MQ=255)                                                                                                        gaAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                               <  1:27920/140‑1 (MQ=255)                                                                                                          aaTCCTGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                             >  2:74280/1‑140 (MQ=255)                                                                                                               tGATCCGGCTATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                        >  2:628428/1‑140 (MQ=255)                                                                                                                        tATTCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCGCCTGCCGGTGc               <  2:518105/140‑1 (MQ=255)                                                                                                                           tCCGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc            <  1:496402/140‑1 (MQ=255)                                                                                                                            ccGAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccc           <  1:348858/140‑1 (MQ=255)                                                                                                                                     aTCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa  >  2:364112/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAA  >  NC_000913/422286‑422558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑