Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,029,739	(A)8→7	coding (231/1191 nt)	rlmI ←	23S rRNA m(5)C1962 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,029,732	0	A	.	100.0%	154.2 / NA	37	coding (238/1191 nt)	rlmI	23S rRNA m(5)C1962 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/18);  total (19/18)

GCACCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGAAAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCT  >  NC_000913/1029594‑1029867                                                                                                                                           |                                                                                                                                        gcaCCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaa                                                                                                                                        >  1:128223/1‑140 (MQ=255)       gAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACACCATTTTGGTGTTTGTTGCAATCGGCGGGaaaaaaag                                                                                                                                  >  1:190404/1‑139 (MQ=255)        aaaaTTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaaaaaaagc                                                                                                                                 <  1:549574/140‑3 (MQ=255)           aTTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAAt                                                                                                                             <  2:67786/140‑1 (MQ=255)               ccGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCg                                                                                                                         >  2:537112/1‑140 (MQ=255)                        tcgatAGTAATACCCCGCAGGCAATTAGAATCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCa                                                                                                                <  1:210855/140‑1 (MQ=255)                          gatAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGa                                                                                                              <  1:91453/140‑1 (MQ=255)                                  tACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCTGG‑AAAAAAAGCAATTTCGATAAACTCAGACGGGTCaa                                                                                                      >  1:211427/1‑140 (MQ=255)                                  tACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCaa                                                                                                      >  1:427747/1‑140 (MQ=255)                                    cccGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAAc                                                                                                    >  1:107795/1‑140 (MQ=255)                                      cGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGGCAAACGt                                                                                                  >  2:162096/1‑140 (MQ=255)                                       ggCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑CAAAGAAGTAATGTCGAGAGACACAGACGGGGCAAACGTc                                                                                                 >  2:201102/1‑140 (MQ=255)                                       ggCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTc                                                                                                 >  1:116071/1‑140 (MQ=255)                                       ggCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGGCAAACGTc                                                                                                 >  2:102346/1‑140 (MQ=255)                                       ggCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGGCAAACGTc                                                                                                 >  1:478682/1‑140 (MQ=255)                                            gCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAAAGTCGATAGACTCAGACGGGTCAAACGTCCAGAc                                                                                            >  1:139151/1‑140 (MQ=255)                                                   gATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGcc                                                                                     <  1:27775/140‑1 (MQ=255)                                                              gATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGa                                                                          >  1:125752/1‑140 (MQ=255)                                                                ttAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAg                                                                        >  2:36532/1‑140 (MQ=255)                                                                 tAAACGATAGCTGTCTAGGCCACCTCTTTCCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGc                                                                       <  1:201102/140‑1 (MQ=255)                                                                    aCGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTgg                                                                    <  2:512110/140‑1 (MQ=255)                                                                         aGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAAt                                                               >  1:53458/1‑140 (MQ=255)                                                                         aGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAAt                                                               >  2:350677/1‑140 (MQ=255)                                                                             gTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAAACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAgc                                                           >  1:18220/1‑140 (MQ=255)                                                                                gAGGCCATCTTTTTGCGCCAGCTAGTCACGCGATTCTTGCGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGcgcc                                                        <  2:211427/140‑1 (MQ=255)                                                                                 aGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGcgccg                                                       <  1:537112/140‑1 (MQ=255)                                                                                       tCTTTTTGCGCCAGCCATTCACCCCATTTTTCTGCATGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCt                                                 <  2:240386/140‑1 (MQ=255)                                                                                        cTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTa                                                >  1:480436/1‑140 (MQ=255)                                                                                              gcgcCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCAtt                                          <  1:478226/140‑1 (MQ=255)                                                                                                   agccagTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTccc                                     <  2:125752/140‑1 (MQ=255)                                                                                                   agccagTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTccc                                     <  2:643712/140‑1 (MQ=255)                                                                                                     ccagTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCtg                                   <  2:116071/140‑1 (MQ=255)                                                                                                        gTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCtgatg                                <  1:162096/140‑1 (MQ=255)                                                                                                        gTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCtgatg                                <  2:478682/140‑1 (MQ=255)                                                                                                        gTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCtgatg                                <  2:427747/140‑1 (MQ=255)                                                                                                              ccATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATcaac                          >  2:604281/1‑140 (MQ=255)                                                                                                               ccattttGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATcaaca                         >  1:339093/4‑140 (MQ=255)                                                                                                                                    cggcggG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAgg    <  2:128223/140‑1 (MQ=255)                                                                                                                                     ggcggG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAggc   <  2:480436/140‑1 (MQ=255)                                                                                                                                      gcggG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAggct  >  1:438770/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        GCACCAGAAAATTACCGAAACGATCGATAGTAATACCCGGCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGAAAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCT  >  NC_000913/1029594‑1029867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑