| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,113,194 | Δ2 bp | coding (2332‑2333/2544 nt) | opgH → | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,113,194 | 0 | C | . | 92.6% | 94.2 / 1.3 | 27 | coding (2332/2544 nt) | opgH | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (15/10); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,113,195 | 0 | G | . | 100.0% | 110.6 / NA | 27 | coding (2333/2544 nt) | opgH | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (15/12); total (15/12) | |||||||||||
TGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGAATTCCCCGGAGAG > NC_000913/1113072‑1113324 || tGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACa > 1:77247/1‑140 (MQ=255) aTCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTACGGTGCTGGAAATCGGC‑‑TGACCCCCACGTTGAACAGgc > 1:218485/1‑140 (MQ=255) ccTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCC‑‑GACCGCCACGTTGAACAGGCGCTGAACGAGACg < 1:462305/140‑1 (MQ=255) atgGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGaa > 2:129068/1‑140 (MQ=255) cTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCC‑‑GACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTg < 1:581465/140‑1 (MQ=255) aGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACTAGATGCTAGAGAAGCTGAATCGCGAtcgt > 1:311618/1‑140 (MQ=255) tttAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGcc > 1:484310/1‑140 (MQ=255) gTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCTCTGAACGACACGCCAGTGAAGCTGAATCGCCATCGTCGCCTGGtgctg > 2:188762/1‑140 (MQ=255) gTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGtgctg > 1:579410/1‑140 (MQ=255) tCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGtgctgc > 1:360758/1‑140 (MQ=255) cATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTgctgct > 2:91053/1‑140 (MQ=255) cATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTgctgct > 2:124500/1‑140 (MQ=255) cATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCGGAACGAGAAGCCAGCGAAGCTGAATCGCGAACGTCGCCTAGTgctgct > 2:309411/1‑140 (MQ=255) ttAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAg < 2:360758/140‑1 (MQ=255) aCGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGa < 2:579410/140‑1 (MQ=255) aCGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGa < 2:89197/140‑1 (MQ=255) tGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGt > 2:85652/1‑140 (MQ=255) cAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGAc < 1:124500/140‑1 (MQ=255) aaTGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGcc > 2:377413/1‑140 (MQ=255) ggCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGt < 1:462782/140‑1 (MQ=255) aCCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGACCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGc > 1:13076/1‑140 (MQ=255) cgTCACCGCGCCAGTAAGGTGCAGGAAATCGGC‑‑TGGCCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTcc < 2:259777/140‑1 (MQ=255) gTCACCGCGCCAGTAAGGTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCg > 2:5686/1‑140 (MQ=255) gcCAGTAAGGTGCTGCAAATCGCC‑‑TCACCCCCACGTTCAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGa < 1:188762/140‑1 (MQ=255) aGTAAGGTGCTGGAAATCGCC‑‑TTACAGCCCCGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGAAtt < 1:305896/140‑1 (MQ=255) gTGCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGAATTCCCCgg < 2:404639/140‑1 (MQ=255) gCTGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGAATTCCCCGgag < 1:85652/140‑1 (MQ=255) tGGAAATCGCC‑‑TGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGAATTCCCCGgagag < 1:5686/140‑1 (MQ=255) || TGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGTTGAACAGGCGCTGAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTGCTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGTGTCTGGAATTCCCCGGAGAG > NC_000913/1113072‑1113324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |